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System purification, detection and treatment

A technology of delivery system and oil body, applied in the field of system purification, detection and treatment, can solve the problems of limited purification technology and other problems

Inactive Publication Date: 2012-10-24
陈致融
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of antibody immobilized immunoaffinity techniques is also limited for purification techniques

Method used

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  • System purification, detection and treatment
  • System purification, detection and treatment
  • System purification, detection and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The oil body carrier of the present invention includes a fusion protein and a lipid, wherein the fusion protein includes an oil body protein (oil body membrane protein, oil body calcin and oil body sterol protein), a ligand peptide, and an antibody Peptide, a cell penetrating peptide, a specific enzyme peptide, a steroid, a sugar, a compound or a combination thereof (a ligand peptide, an antibody peptide, a cell penetrating peptide, a specific enzyme peptide, a Steroids, a sugar or a compound are referred to as specific peptides for recognizing diseased cells, also known as specific peptides).

[0054] The fusion protein in the oil body carrier of the present invention does not have any limitation on the oil body protein contained therein. However, the oleosin is an oleosin belonging to a plant seed, for example an oleosin of a plant seed selected from the group: sesame, olive, soybean, peanut, sunflower, canola, flax, safflower, Or a combination thereof; optimally, th...

Embodiment 2

[0066] [Example 2] separation and purification

[0067] The present invention uses genetic engineering technology to separate and purify the target oil body carrier, which contains an oil body protein (oil body membrane protein, oil body calcin and oil body sterol protein), a target protein peptide, a specific linker and a lipid . Wherein, the specific linker can be hydrolytically cleaved by a specific specific protease to obtain the target protein.

[0068]The separation and purification of the target protein peptide of the target oil body carrier in the present invention is not subject to any limitation, and the peptide can be selected from the following groups: medicinal proteins (for example: hirudin, insulin, lycopene, epidermal growth factor, apolipoprotein A1 ( Apo-A1), apolipoprotein B (Apo-B) or combinations thereof), industrial enzymes (such as β-glucuronidase (β-Glucuronidase), xylanase (xylanase), rennet (rennet) , glucose isomerase, phosphate isomerase, collagen...

Embodiment 3

[0073] [Example 3] Vaccine

[0074] The invention utilizes genetic engineering to form an antigen oil body carrier, which has the ability to carry the antigen to trigger an immune response to generate antibodies, and to produce animal vaccines. The oil body carrier includes an oil body protein (oil body membrane protein and oil body calcin), an antigen protein and a lipid.

[0075] The present invention utilizes the antigen oil body carrier, wherein, the antigenic protein is not subject to any limitation, and the protein can trigger an immune response to produce antibodies to produce animal vaccines: antigens of oral vaccines (such as polio, oral rotavirus or a combination thereof), injection vaccines Antigens of influenza (such as influenza, Japanese encephalitis, hepatitis A, rabies or combinations thereof), antigens of immune vaccines (such as hepatitis A or B, rubella, chickenpox, rabies, tetanus or combinations thereof) or combination.

[0076] The lipid in the antigen ...

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Abstract

According to the present invention, an oil body carrier is assembled through genetic engineering, and can be used in specificity detection of the oil body carrier and targeted therapy; oleosin or caleosin is combined to form an oil body vector system to carry out purification so as to rapidly and economically obtain target protein. With the present invention, an antigen oil body carrier of the oleosin or the caleosin is adopted to carry out an animal immune reaction so as to obtain a vaccine.

Description

technical field [0001] The present invention utilizes genetic engineering to link the oil body protein with a specific peptide, and connect it with a drug peptide and / or a fluorescent peptide to assemble an oil body carrier. The oil body can be coated with drugs, known fluorescent signal molecules or / or and bacteria, and used in the specific detection of oil body carriers or / and the application of targeted therapy. [0002] The invention utilizes the oil body system in which the oil body protein is linked with the target peptide to purify, and obtains the high-purity target protein for application in various fields. [0003] The invention utilizes the antigen oil body carrier of the oil body protein to carry out animal immune reaction, the antigen triggers the immune reaction to generate antibodies, takes out the antibodies, and forms a vaccine. Background technique [0004] Cancer is currently the main killer that threatens human life and health around the world. According...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/44A61K47/42A61K39/00A61K39/395A61K49/00A61P35/00
CPCA61K31/475A61K47/44A61K38/00A61P35/00Y02A50/30
Inventor 陈顺基朱宝美
Owner 陈致融
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