Vegetable oil skin care lotion containing basic fibroblast growth factor active peptide

A technology of growth factor activity and fibroblasts, which is applied in the fields of biotechnology and cosmetics, can solve the problems of low survival rate and increased survival rate of nerve cells, and achieve the effects of simple process, reduced cost and reduced production cost

Inactive Publication Date: 2013-10-02
JILIN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nerve transplantation has broad prospects in the treatment of neurological diseases, but the survival rate of transplanted nerve cells is not high. It has been reported that pretreatment of nerve cells with bFGF before transplantation can increase the survival rate

Method used

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  • Vegetable oil skin care lotion containing basic fibroblast growth factor active peptide
  • Vegetable oil skin care lotion containing basic fibroblast growth factor active peptide
  • Vegetable oil skin care lotion containing basic fibroblast growth factor active peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Synthesis of Basic Fibroblast Growth Factor Gene

[0036] According to the preference of plant codons, the nucleotide sequence of basic fibroblast growth factor bFGF found in GENBANK was modified, and the rare amino acid codons with less content in plants were replaced with plant-preferred codons. According to the degeneracy of codons, the enzyme cutting sites used in this experiment were eliminated, and then sent to Shanghai Sangon Bioengineering Co., Ltd. for artificial synthesis of recombinant basic fibroblast growth factor gene (SEQ ID NO.1).

Embodiment 2

[0037] Example 2: Cloning of kidney bean seed-specific promoter and terminator

[0038] To clone the promoter and terminator genes of kidney bean, in order to clone the seed-specific promoter and terminator from the genome sequence of kidney bean, using primers, the standard polymerase chain reaction (PCR) was used to amplify the 1548bp and 1220bp genes from the genome of kidney bean DNA fragments, the amplified fragments were digested with restriction endonucleases, cloned into the pUC57 cloning vector, and stored for future use. After sequencing, its base sequence is shown in the sequence table SEQ ID NO.2 and SEQ ID NO.3.

[0039] bean promoter primer

[0040] Primer5F: GAATTCATTGTACTCCCAG

[0041] Primer5R: AGTAGAGTAGTATTGAATATGAG

[0042] bean terminator primer

[0043] tem5F: AATAAGTATGAACTAAAATGC

[0044] tem5R: TTAGTTGGTAGGGTGCTAGGAA

Embodiment 3

[0045] Example 3: Construction of plant-specific expression vector pOBT

[0046] The cloning vector of pUC57-bean promoter was digested with restriction endonucleases pstI and NcoI, and the target fragment of the bean promoter sequence was recovered. At the same time, the basic plasmid pO was digested with pstI and NcoI (taking p1301 as the basic skeleton, and its T-DNA region Transformation was carried out, except for the NOS gene that retained the T-DNA region, the rest of the genes were replaced by 35S and Bar genes, using the pEGAD plasmid as a template, using the 35S-F upstream primer 47bp cggggtaccAtccgtcaacatggtggagcacgacacgcttgtctact and the Bar-R downstream primer 54bp cggaattcactagttcagatctcggtgacgggcaggaccggacggggcggaacc for PCR amplification. The 35S-Bar gene was amplified, and inserted into the front of the NOS gene in the T-DNA region of the pCAMBIA1301 vector using the kpnI and SpeI restriction sites to construct the p1301-35S-Bar-NOS basic plasmid (abbreviated...

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Abstract

The invention discloses a vegetable oil skin care lotion containing basic fibroblast growth factor active peptide. A fusion expression product of oleosin which is inlaid in the surface of oil body and basic fibroblast growth factor is used as an active ingredient in the development of skin care products; so that mixing technologies of basic fibroblast growth factor with an emulsifier (or a protective agent) are simplified, and production cost is reduced. In addition, the bioactivity of basic fibroblast growth factor protein is protected. By taking advantage of the characteristics of oleosin, basic fibroblast growth factor active peptide is encapsulated with vegetable oil, and is fixed on the surface of oil body; and then basic fibroblast growth factor protein can be distributed uniformly in the skin care emulsifier by using emulsifying homogenization; and the effects of basic fibroblast growth factor protein are exerted by transdermal absorption effects of oil body. Preparation technologies of the vegetable oil skin care lotion are simple; efficacy is significant; and cost is reduced.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and cosmetics, and in particular relates to a vegetable oil body containing basic fibroblast growth factor active polypeptide and a vegetable oil body skin care emulsion containing basic fibroblast growth factor active polypeptide. Background technique [0002] Basic fibroblast growth factor (bFGF) is a protein polypeptide with a very small content in the human body. It plays a very important role in human life activities and has a huge role in medical treatment. In the 1940s, Hoffman and colleagues discovered that substances contained in the pituitary gland and brain stimulate the growth of fibroblasts. In 1974, Gospodarowiez et al. first isolated and purified the active substance from the bovine pituitary gland. Because it can obviously promote the division and growth of fibroblasts, they named it fibroblast growth factor. [0003] Clinical application of bFGF: [0004] There are abunda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/10A61K8/92A61K8/64A61Q19/00
Inventor 李海燕李校堃杨晶王法微杜林娜官丽莉金立波姜潮郭咏昕董圆圆王南王艳芳刘秀明姚娜
Owner JILIN AGRICULTURAL UNIV
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