A kind of method and germination agent for promoting direct seeding germination of bletilla striata seeds

A technology for seeds and Bletilla striata, which is applied in the directions of seed and rhizome treatment, germination equipment, calcium fertilizer, etc., can solve the problems of large seed consumption, large labor force, and difficulty in seedling cultivation.

Active Publication Date: 2022-03-11
重庆市药物种植研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the division propagation period is long, the reproduction efficiency is low, and the consumption of seeds is large, and the labor force is large, so it is difficult to meet the needs of mass cultivation.
Bletilla striata can also be propagated by sowing, but the seeds of Bletilla striata are very small and have no endosperm, so it is difficult to germinate and grow under natural conditions, and the cultivation of seedlings is more difficult

Method used

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  • A kind of method and germination agent for promoting direct seeding germination of bletilla striata seeds
  • A kind of method and germination agent for promoting direct seeding germination of bletilla striata seeds
  • A kind of method and germination agent for promoting direct seeding germination of bletilla striata seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Seed vitality test:

[0045] Seed vigor is detected by TTC method: the embryos of living seeds can undergo redox reactions during respiration, while dead seeds do not. When TTC penetrates into living seed embryo cells as a hydrogen acceptor and is reduced by hydrogen on dehydrocoenzyme (NADH2 or NADPH2), colorless TTC turns into red triphenylmethylhydrazone (TTF); if the seed embryo dies or If the viability of the seed embryo declines, it cannot be dyed or the dyeing is relatively light. Therefore, the viability of the seed can be identified according to the dyed part of the seed embryo or the degree of dyeing depth.

[0046] The specific steps are: (1) randomly select 10 Bletilla striata seeds, use tweezers to break the shell and take out the seeds, and mix the seeds of the 10 seeds; (2) randomly take 100 seeds and place them in 4 petri dishes , Soak overnight in clean water in a 25°C incubator to make the seeds fully imbibed. (3) Air-dry the surface moisture of the im...

Embodiment 2

[0052] Effects of different germination media on germination rate, protocorm differentiation rate, germination medium contamination rate.

[0053] Effects of Different Media on the Germination Rate of Bletilla striata Seeds Directed

[0054] Select 4 types and 10 kinds of germination media (soil: sandy loam, fine sand, red jade soil, rock: vermiculite, perlite, organic matter: coconut peat, fungus bag, dead leaf powder, fiber: cotton, cotton gauze ). The germination rate was determined by the seed dish germination method. Take an appropriate amount of the above-mentioned media and place them in a 90mm petri dish, level the surface, and spray clean water to fully soak the media. Each medium was repeated 3 times, and a double-layer filter paper with inner sponge was set as a control. Randomly select 10 capsules of Bletilla striata, cut off the pericarp, shake out the seeds, mix well, and soak them overnight in clean water in an incubator at 25°C to make the seeds fully imbibe...

Embodiment 3

[0101] Effects of Different Nutrient Solution Formulas on Germination Rate, Differentiation Rate and Seedling Rate of Bletilla striata Seeds

[0102] Using L9(34) orthogonal design, the four influencing factors are: A (MS nutrient solution), B (NAA), C (GA3), D (sucrose), each factor is set to three levels and marked as L1\ L2\L3.

[0103] Table 14. Orthogonal experiment factors and levels

[0104]

[0105] Table 15. Orthogonal experimental design of different nutrient solution formulations

[0106]

[0107] 14 days, 28 days, and 56 days after sowing, observe and record the number of germinated seeds with a magnifying glass, and count the germination rate, protocorm differentiation (differentiation, undifferentiation) rate and seedling rate (plant height reaching 3 cm is regarded as a seedling)

[0108] Orthogonal design and analysis of variance were performed using SPSS 19.0 software. The seedling rate is used as a reference index for the group, and the optimal nutri...

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Abstract

The invention belongs to the technical field of plant cultivation, and in particular relates to a medium capable of promoting direct-directed germination of bletilla striata seeds and direct-directed seedling growth, and an optimal selection and germination method of nutrients and environmental conditions. The medium selection of the present invention that can improve the direct seeding germination rate of bletilla striata seeds includes: sandy loam, fine sand, red jade soil; vermiculite, perlite; coconut bran, bacteria bag, dead leaf powder; cotton, cotton gauze. The selected germination nutrient solution components are: 1 / 4MS~MS nutrient solution, 0.5mg / L~2mg / L NAA, 0mg / L~1mg / L GA3, 10~30g / L sucrose. The germination temperature is selected to be 15-25°C, the relative humidity is 50-90%, the light intensity is 1000-3000 lx, and the light duration is 8-16 hours / day. The combination of the medium and the nutrient solution can effectively improve the direct seeding germination rate of bletilla striata seeds.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for improving the germination rate by optimizing the germination medium, nutrient solution and germination environment of bletilla striata seeds for direct seeding germination through experiments, Background technique [0002] Bletilla striata, also known as Lianjicao, Gangen, etc., is a perennial herbaceous bulbous plant with a plant height of 18-60 cm. Mainly distributed in China, Japan and northern Myanmar. Bletilla striata has a wide range of medicinal value and garden value. It can be used to restrain bleeding, reduce swelling and promote muscle growth. It is used for hemoptysis and hematemesis, traumatic bleeding, sore swelling, chapped skin; pulmonary tuberculosis hemoptysis, ulcer disease hemorrhage; it can also be used in health-preserving medicinal diet, tubers contain mucus and starch, etc., and can be used as paste. As a kind of ground orchid, bl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/02C05G3/00C05G5/20
CPCA01C1/00A01C1/025C05D3/00C05G3/00C05G5/20C05D9/00C05D9/02C05F11/00C05F11/10
Inventor 任风鸣刘艳卓维张万超
Owner 重庆市药物种植研究所
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