A kind of method of ginger tissue culture

Abstract

A method for rapidly propagating ginger through tissue culture, characterized in that: the shoot tip of ginger is pretreated and placed on a slow-release medium to induce clustered buds, and then NH is added to the slow-release medium 4 NO 3 MS basal medium with half the concentration, and supplemented with 0.5~1mg / L PP 333 , continuous culture to obtain rooted shoots; the slow-release medium is supplemented with 6-BA 0.5~1.5mg / L, NAA slow-release auxin 2~3mg / L, TDZ 0.25~0.3mg / L in the MS basal medium, 2% sucrose, 0.55~0.65% agar, adjust the pH to 5.8~6. The invention simplifies the tissue culture steps, shortens the culture time, improves the survival rate and detoxification rate of sprouts, the survival rate reaches 96%, and the detoxification rate reaches 100%, and simultaneously prevents the variation and multiplication in the ginger tissue culture process. The coefficient has been significantly improved, reaching 5.13, and the rooting rate has reached 97.7%, which can cultivate robust rooted ginger seedlings.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of ginger. Background technique [0002] Ginger is a perennial herbaceous plant of the Zingiberaceae Zingiberaceae that lives for one year, and is the main source of planting in many areas of my country. Ginger is an economical plant with both medicinal and edible uses. It has the functions of sweating and relieving the exterior, warming the middle and relieving vomiting, warming the lungs and relieving cough, and detoxifying fish and crabs. China is the birthplace and one of the main producing countries of ginger, with a cultivation history of more than two thousand years. The traditional ginger production is to select strong, disease-free rhizomes in the harvest season and store them in a cellar. The next year, the germinated ginger is cut into pieces for planting. This method has serious waste and requires a lot of manpower and material reso...

AI Technical Summary

Problems solved by technology

But existing tissue culture is not suitable for the tissue culture of ginger, it is difficult to induce it to produce callus tissue and clustered buds, slow propagation, easy browning of callus tissue, low induction survival rate, easy rotten seedlings, and loaded down with trivial details Variations are prone to occur during the cultivation process, resulting in problems such as low reproduction coefficient, low rooting rate, and deformed seedlings in ginger tissue culture.

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