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Dendrobium officinale domesticated breeding cultivation method

A Dendrobium officinale, domestication and propagation technology, applied in the field of plant cultivation, can solve the problems of slow differentiation, difficult industrial reproduction, low reproduction efficiency, etc., and achieve the effects of simplifying the cultivation steps, shortening the cultivation period and reasonable design

Active Publication Date: 2014-04-16
杭州富阳文曲生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many methods for cultivating Dendrobium officinale, but they generally have problems such as low reproductive efficiency, slow differentiation, and difficulty in industrial reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Take 4-month-old seeds produced by domesticated Dendrobium officinale, and disinfect them with alcohol;

[0019] 2) Sow the seeds obtained in step 1) on the protocorm induction medium for cultivation. The protocorm induction medium is MS medium + 8% banana puree. The cultivation conditions are: temperature 22°C, humidity 38%, light The condition is 2200LUX, the light time is 12h / d, and the culture is 1 month;

[0020] 3) Put the protocorms into the differentiation medium for cultivation. The differentiation medium is MS medium + 8% banana mash + 10% mashed potato + NAA0.5mg / L. The culture conditions are: temperature 26°C, humidity 45%, the light condition is 1800LUX, the light time is 12h / d, and the culture is 18 days;

[0021] 4) Cut the cluster buds induced to differentiate into single buds, and then put them into the rooting medium for cultivation. The rooting medium is MS medium + 8% banana puree + IBA0.5mg / L + NAA0.25mg / L, The culture conditions are: temperatu...

Embodiment 2

[0024] 1) Take the 5-month-old seeds produced by the domesticated Dendrobium candidum, and disinfect them with alcohol;

[0025] 2) Sow the seeds obtained in step 1) on the protocorm induction medium for cultivation. The protocorm induction medium is MS medium + 7% banana puree. The cultivation conditions are: temperature 21°C, humidity 40%, light The condition is 2000LUX, the light time is 12h / d, and the culture is 1.5 months;

[0026] 3) Put the protocorms into the differentiation medium for cultivation. The differentiation medium is MS medium + 7% banana mash + 11% mashed potato + NAA0.5mg / L. The culture conditions are: temperature 24°C, humidity 40%, the light condition is 1500LUX, the light time is 12h / d, and the culture is 15 days;

[0027] 4) Cut the cluster buds induced to differentiate into single buds, and then put them into the rooting medium for cultivation. The rooting medium is MS medium + 7% banana puree + IBA0.5mg / L + NAA0.25mg / L, The culture conditions are: ...

Embodiment 3

[0030] 1) Take the 6-month-old seeds produced by the domesticated Dendrobium candidum, and disinfect them with alcohol;

[0031] 2) Sow the seeds obtained in step 1) on the protocorm induction medium for cultivation. The protocorm induction medium is MS medium + 9% banana puree. The cultivation conditions are: temperature 23°C, humidity 35%, light The condition is 2500LUX, the light time is 12h / d, and the culture is 2 months;

[0032] 3) Put the protocorms into the differentiation medium for cultivation, the differentiation medium is MS medium + 9% banana mash + 12% mashed potato + NAA0.5mg / L, the culture conditions are: temperature 28°C, humidity 50%, the light condition is 2000LUX, the light time is 12h / d, and the culture is 20 days;

[0033] 4) Cut the cluster buds induced to differentiate into single buds, and then put them into the rooting medium for cultivation. The rooting medium is MS medium + 9% banana puree + IBA0.5mg / L + NAA0.25mg / L, The culture conditions are: te...

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Abstract

A Dendrobium officinale domesticated breeding cultivation method belongs to plant cultivation method. The method comprises the following steps: 1) disinfecting seeds produced by domesticated Dendrobium officinale with alcohol; 2) conducting induction culture on protocorm; 3) conducting differentiation culture; 4) conducting rooting culture; and 5) conducting hardening-seedling treatment, and planting in a greenhouse. The Dendrobium officinale domesticated breeding cultivation method has reasonable design; different culture media and culture conditions are employed for culture in different stages, so as to realize a final average rooting rate of 98% and an average transplanting survival rate of 97%, shorten culture cycle, and simplify culture steps.

Description

technical field [0001] The invention belongs to a plant cultivation method, in particular to a method for domesticating, breeding and cultivating dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of Orchidaceae. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes warm and humid climates and semi-shady and semi-yang environments, and is not cold-resistant. Generally, they can withstand low temperatures of -5°C. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. Dendrobium candidum has unique medicinal value, and its stem is used as medicine. The name of the traditional Chinese medicine: Dendrobium, belongs to the yin-tonifying medicine in the tonic medicine. "Chinese Pharmacopoeia": Yiwei Shengjin, nourishing yin and clearing heat. [0003] There are ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡士宏
Owner 杭州富阳文曲生态农业开发有限公司
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