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Method for rapid propagation of zephyr lily

A fast technology of onion orchid, which is applied to the field of rapid propagation of terminal buds of onion orchid in vitro, can solve the problems of harsh material selection and method of onion orchid, can not meet market demand, cannot achieve rapid reproduction, etc. The effect of small variation and fast reproduction

Inactive Publication Date: 2012-04-18
ZHENGZHOU NORMAL UNIV BIOLOGY ENG RES
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  • Abstract
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Problems solved by technology

The results of these studies are not up to the purpose of rapid reproduction
At the same time, due to the relatively small bulb discs of onion orchids, the cutting method of bulb discs cannot be used for cultivation, and the reasons for the aforementioned diseases of onion orchids make the selection of materials and methods for rapid propagation of onion orchids more demanding and difficult. Can not meet a large number of market needs

Method used

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  • Method for rapid propagation of zephyr lily
  • Method for rapid propagation of zephyr lily

Examples

Experimental program
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Embodiment 1

[0024] The first step is to take the cultivated onion orchid plant, wash the soil with tap water, cut off the root system and leaves, and rinse the remaining bulbs under tap water for 4 hours; peel off the outer 2 / 3 of the scale leaves and axillary buds of the bulb, and then use the Soak in 75% alcohol for 2 minutes, rinse with sterile water for 5 times, then soak in mercuric chloride solution with a mass fraction of 1‰ for 12 minutes, then rinse with sterile water for 5 times, cut off the sterilized bulb disc base, Inoculate the terminal buds with a remaining diameter of about 0.6cm in the induction medium, the medium is MS basic medium, the additives are: 0.5mg / L TDZ and 0.1mg / L NAA, add 30g / L sucrose, 8g agar / L, the pH value of the medium is 5.9, the culture conditions are: temperature 25±2°C, the light time is 12 hours / day, the light intensity is 2500Lux, and the induction time of adventitious buds is 18 days. figure 1 ;

[0025] The second step is to cut the differentia...

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Abstract

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.

Description

technical field [0001] The invention relates to a tissue culture method in the field of biotechnology, in particular to a rapid propagation method for in vitro culture of terminal buds of scallions orchids. Background technique [0002] Onion orchid (Zephyranthes candida herb.), a plant of the genus Amaryllidaceae, also known as onion lotus, jade curtain, and white calamus; it is native to South America. It is a perennial herbaceous bulb with a conical bulb and a slender neck; the plant height is 15-20cm. Leaves are basal, linear, slightly fleshy, tufted, dark green. Scapes are hollow, pulled out from the leaves, solitary, white, with six perianths; stamens are bright yellow or golden yellow, elliptic-lanceolate, without tube. The flower diameter is 3-4cm. The flowering period is from late June to early November. The plants of onion orchids are low and neat, easy to survive, with many flowers, long flowering period, clean and elegant flowers, rich fragrance, tranquil and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 袁秀云谢惠玲季洪强
Owner ZHENGZHOU NORMAL UNIV BIOLOGY ENG RES
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