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Tissue culture device and betula luminifera micro cuttage and rapid propagation method

A tissue culture device and micro-cutting technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of incomplete maintenance of plant root system, plant death, etc., achieve good germination and rooting conditions, shorten the growth cycle, The effect of reducing variation

Inactive Publication Date: 2014-12-10
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional micro-cutting medium is all solid medium. If the medium needs to be replaced during the cultivation process and the integrity of the plant needs to be maintained, it cannot be achieved by using solid medium, especially for those fibrous plants. Take out the plant, the root system of the plant cannot be kept intact, and it may even lead to the death of the plant

Method used

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  • Tissue culture device and betula luminifera micro cuttage and rapid propagation method
  • Tissue culture device and betula luminifera micro cuttage and rapid propagation method
  • Tissue culture device and betula luminifera micro cuttage and rapid propagation method

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Experimental program
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Effect test

Embodiment 1

[0029] figure 1 The structure schematic diagram of the tissue culture device provided by the present invention includes a culture vessel main body 1 and a cover 5, and the culture vessel main body 1 and cover 5 are prior art, and will not be repeated here. Different from the prior art, the present invention is provided with a fixed piece 3 in the container main body 1, and the lower part of the culture vessel main body 1 is a semi-solid medium 2, and the fixed piece 3 is positioned on the upper surface of the semi-solid medium 2, and the fixed piece 3 There is an opening 7 for inserting the plant explant 2, and the explant is fixed on the fixing sheet. The fixed sheet 3 can be made by punching holes with filter paper, or other materials can be used. The fixed sheet needs to meet the following requirements: on the one hand, it can fix the explant, and on the other hand, it will not limit the growth of the plant. Using this device for culture, when the culture medium needs to ...

Embodiment 2

[0032] When adopting the device in embodiment 1 to carry out the rapid propagation of birch miniature cuttings, the operation steps are as follows:

[0033] 1) Prepare a semi-solid medium and pour it into a culture container. After autoclaving, the medium is naturally cooled to a semi-solidified state;

[0034] 2) Place the high-temperature sterilized filter paper on the surface of the semi-solid medium in the culture container on the sterile operating table;

[0035] 3) Select aseptic seedlings of the same clone of Betula glabra with consistent growth, cut off the 3-4 and 5-6 stem segments on the aseptic operating table, and leave half a leaf on the top of each explant;

[0036] 4) Insert the morphological lower end of the explant into the semi-solid medium through the filter paper hole, straighten it, tighten the cover of the culture container, and place it on the culture rack;

[0037] 5) Put it into the cultivation room for light cultivation, keep the temperature at 23-27°C...

Embodiment 3

[0039] Embodiment 3: Betula glabra semi-solid medium formula

[0040] Using MSB5 semi-solid medium, the specific formula is as follows:

[0041] MSB5 semi-solid medium (1L)

[0042] MS large amount of mother liquor (20×) 50ml

[0043] MS trace stock solution (20×) 50 ml

[0044] MS iron salt mother liquor (20×) 50 ml

[0045] B5 organic mother liquor (100×) 10 ml

[0046] Sucrose 30 g

[0047] Agar powder 2.8 g

[0048] pH adjusted to 5.9

[0049] The specific mother liquor formula is as follows:

[0050] NH 4 NO 3 33g KNO 3 38g K H 2 PO 4 3.4g CaCl 2 2H 2 o 8.8g MgSO 4 ·7H 2 o 7.4g

[0051] FeSO 4 ·7H 2 o 5.56g Na 2 EDTA·2H 2 o 7.46g

[0052] KI 0.166g h 3 BO 3 1.24g MnSO 4 4H 2 o 4.46g ZnSO 4 ·7H 2 o 1.72g Na 2 MoO 4 2H 2 o 0.05g CuSO 4 ·5H 2 o 0.005g CoCl 2 ·6H 2 o 0.005g

[0053] Inositol 10g niaci...

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Abstract

The invention relates to a tissue culture device, belonging to the field of biotechnology. The tissue culture device comprises a culture container main body and a cover, and is characterized in that a semi-solid culture medium is arranged in the lower part of the culture container main body, a stator is arranged on the upper surface of the semi-solid culture medium, and open pores are formed in the stator and used for cutting explants. The invention further provides a betula luminifera micro cuttage and rapid propagation method by combining the device. The method is simple in steps, the growing speed is high, and the occurrence of variation can be reduced. Through the method, in-vitro sterile culture can be realized, and a great amount of plants can be propagated with fewer explant materials within small range and limited culture time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a tissue culture device and the use of the culture device for rapid propagation of betula miniature cuttings. Background technique [0002] The micro-cutting technique refers to the in vitro culture of a stem segment with one bud, so that the terminal bud or axillary bud develops into a seedling. It is very similar to traditional cuttings, but micro cuttings generally do not need to add exogenous hormones to break the apical dominance, and sometimes a small amount of auxin can be added, which not only promotes the germination of axillary buds, but also produces better rooting effects. The biggest advantage of the micro-cutting technique is to reproduce as many plants as possible with as little explant material as possible in a small range and within a limited culture time through aseptic culture in vitro. In recent years, micro-cutting technology has been used in tea trees ( Camellia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张俊红吴骏童再康
Owner ZHEJIANG FORESTRY UNIVERSITY
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