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Method for quickly breeding cymbidium hybridium by use of root inducing protocorm

A protocorm and large flower technology, applied in the field of plant cell engineering, can solve the problems of high cost and tediousness, and achieve the effects of low cost, high planting survival rate and simple induction medium

Inactive Publication Date: 2012-10-31
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0005] So far, the process of tissue culture of Cymbidium has been relatively cumbersome and costly. In order to improve efficiency, reduce costs and carry out rapid propagation, the focus of future research should be to simplify the propagation process and avoid induction, subculture, and seedling cultivation as much as possible. Using different media for the four stages of rooting and rooting, to explore a method of tissue culture of Cymbidium that can reduce variation and increase the proliferation coefficient, and the regenerated plants grow robustly

Method used

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  • Method for quickly breeding cymbidium hybridium by use of root inducing protocorm
  • Method for quickly breeding cymbidium hybridium by use of root inducing protocorm
  • Method for quickly breeding cymbidium hybridium by use of root inducing protocorm

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Embodiment 1

[0032] (1) Preparation of medium: MS basic medium 1L + sucrose 30000mg / L + agar powder 5000mg / L + special ingredients (6-BA 0.5mg / L + PIC 0.5mg / L + hydrolyzed casein 500mg / L). After preparation, adjust pH=5.4-5.6, sterilize at 121±2°C, 15-20 pounds for 20 minutes.

[0033] (2) Select the root of the aseptic seedling of Cymbidium cymbidium, inoculate it in the medium prepared in step (1) for dark culture, the temperature is 25±2°C, the root expands in 15 days, and the protocorm will be produced in about 1 month (such as figure 1 Shown), put it under the light and cultivate it, the temperature is 25±2℃, and the light is 16 hours a day, and it will gradually turn green (such as figure 2 shown).

[0034] ⑶ Cut off the protocorms (50 protocorms are produced from an average root of 2 cm in length, the induction rate is 100%, and the protocorm survival rate is 95%) and inoculate them in step ⑴ without special ingredients (such as image 3 shown), after 1 month of cultivation, it g...

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Abstract

The invention discloses a method for quickly breeding cymbidium hybridium by use of root inducing protocorm. The improved MS culture medium containing special components (6-BA, PIC and casein hydrolysate) is used for inducing protocorm; for the root explant of cymbidium hybridium, the protocorm is induced by the root of cymbidium hybridium through a special cultivation method, wherein the induction rate reaches 100%; and after 45 days of cultivation, 50 protocorms of each root explant are obtained, the survival rate of the protocorm is 95%, and the plant grows well and directly roots.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and relates to a method for rapidly propagating Cymbidium cymbidium by using roots to induce protocorms. Background technique [0002] Cymbidium hybridium (Cymbidium hybridium) is a perennial herb of Orchidaceae. It is a high-end indoor potted flower and high-end cut flower popular in the international flower market in recent years, because of its beautiful color and long flowering period (February to March ), and it is open during the Winter and Spring Festival, so it has a very high ornamental value. Cymbidium is mostly a hybrid variety, and seed propagation cannot maintain its variety characteristics, and the seed setting rate is relatively low. Therefore, the traditional cultivation relies on branch reproduction, so the cycle is long, the reproduction coefficient is low, and the reproduction speed is slow, which is far from meeting the requirements of commercial production. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周音张建军殷丽青谢纪红
Owner SHANGHAI ACAD OF AGRI SCI
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