Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof

A Dendrobium nobile and culture medium technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of cumbersome three-step seedling formation, reduction in the number of tissue culture seedlings, waste of manpower, financial and material resources, etc.

Active Publication Date: 2020-11-13
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0003] At present, Dendrobium seedlings are mainly obtained through plant tissue culture, although the existing tissue culture technology has been continuously improved, and a three-step seedling technology with seeds as explants has been formed, that is, the seeds are germinated and cultured for 2 to 3 months before transfer. After being cultured for 3 to 4 months in the growth medium for strong seedlings and then transferred to the rooting medium for 2 to 3 months, the three-step seedling formation method is still cumbersome and requires multiple bottle changes, which not only wastes manpower , financial and material resources, and increased the pollution rate of tissue culture seedlings, resulting in a decrease in the number of tissue culture seedlings and loss of test tube seedlings

Method used

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  • Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof
  • Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof
  • Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Rapid propagation of Dendrobium nobile seedlings

[0040] (1) Selection of pods: On March 15, 2019, select pods with plump fruits, no pests and diseases, and no ruptured capsules. The best time to collect materials is at noon on a sunny day.

[0041] (2) Disinfection of the capsules of Dendrobium nobile: Take the mature capsules of Dendrobium nobile, wash them under running water for 65 minutes, soak them in washing powder for 23 minutes, continue to rinse them with running water, and disinfect them with 75% ethanol for 30 seconds on the ultra-clean workbench. Rinse with water three times, then sterilize with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 to 6 times, and absorb the surface moisture with filter paper.

[0042] (3) Preparation of medium: preparation of one-step seedling medium, add 2.47g of N6 basic medium to 600mL of tap water, add 1.0mg / L 6-BA, 0.3mg / L2,4-D, add crushed 45g of potato juice, 145g of banana juice, and 25g of sucrose...

Embodiment 2

[0046] The cultivation process is the same as the embodiment, except that the concentration of the potato juice in the one-step seedling growth medium is 30g / L, and the concentration of the banana juice is 130g / L.

Embodiment 3

[0048] The cultivation process is the same as the embodiment, except that the concentration of the potato juice in the one-step seedling growth medium is 60g / L, and the concentration of the banana juice is 160g / L.

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Abstract

The invention provides a culture medium for rapid propagation of dendrobium nobile seedlings and a rapid propagation method thereof, and belongs to the technical field of plant seedling cultivation. The culture medium for rapid propagation of dendrobium nobile seedlings is prepared from the following components in percentage by mass and volume concentration: 2.35 to 2.68 g / L of N6 basic culture medium, 1.0 to 1.5 mg / L of 6-BA, 0.3 to 0.5 mg / L of 2,4-D, 30 to 60g / L of potato juice, 130 to 160g / L of banana juice, 20 to 30g / L of cane sugar and 6 to 7g / L of agar, wherein the pH value of the culture medium is 5.6 to 5.8. When the culture medium for rapid propagation of the dendrobium nobile seedlings is used for culturing the dendrobium nobile seedlings, transferring is not needed, the seedlings are formed at a time, pollution caused by frequent transferring is avoided, the pollution rate is reduced, and the seedling culture cost is greatly saved.

Description

technical field [0001] The invention belongs to the technical field of plant seedling cultivation, and in particular relates to a medium for rapid propagation of Dendrobium nobile seedlings and a rapid propagation method thereof. Background technique [0002] Dendrobium nobiLeLindL (Dendrobium nobiLeLindL) is a perennial epiphytic herb of the genus Dendrobium in the family Orchidaceae. It has the functions of human immunity, nourishing the five viscera and fatigue, etc., and it is a traditional Chinese medicine in my country. [0003] At present, Dendrobium seedlings are mainly obtained through plant tissue culture, although the existing tissue culture technology has been continuously improved, and a three-step seedling technology with seeds as explants has been formed, that is, the seeds are germinated and cultured for 2 to 3 months before transfer. After being cultured for 3 to 4 months in the growth medium for strong seedlings and then transferred to the rooting medium fo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 向增旭张成才覃芳罗丽娜张子璇
Owner NANJING AGRICULTURAL UNIVERSITY
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