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Phalaenopsis tissue culture media and preparation methods thereof

A technology of tissue culture medium and culture medium, applied in the field of plant tissue culture, which can solve the problems of low plant mutation rate and easy browning of plants, and achieve the effects of low mutation rate, simplified cultivation steps, and reduced production costs

Inactive Publication Date: 2018-08-21
内蒙古自治区生物技术研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the problems existing in the above-mentioned prior art, provide a kind of Phalaenopsis tissue culture medium and preparation method thereof, it simplifies the cultivation step, pedicel survival rate is high, plant variation rate is low, effectively solves the problem of Phalaenopsis The technical problem that the plants are easy to brown in the process of tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: a kind of Phalaenopsis tissue culture medium, it comprises induction medium, proliferation medium, strong seedling medium and rooting medium;

[0035] The specific formulation of the induction medium is as follows: 1 / 4MS medium 1339mg / L, thiadiazide urea 3mg / L, peptone 2000mg / L, citric acid 30mg / L, sucrose 30000mg / L, agar 6000mg / L, 6 - Benzylaminopurine 3mg / L, coconut milk 100mg / L, distilled water needed to set the volume to 1L;

[0036] The proliferation medium is composed of the following components: Huabao No. 1 500mg / L, Huabao No. 2 1500mg / L, inositol 100mg / L, multidimensional element tablet (29) 300mg / L, peptone 1000mg / L, tomato juice 18000mg / L, carrot puree 18000mg / L, apple puree 18000mg / L, banana puree 18000mg / L, coconut milk 100mg / L, rosemary extract 8mg / L, polyvinylpyrrolidone 1000mg / L, adenine 1mg / L, 6 - Benzylaminopurine 1mg / L, naphthaleneacetic acid 1mg / L, sucrose 20000mg / L, agar 6000mg / L, distilled water needed to set the volume to 1L;

[0...

Embodiment 2

[0039] Embodiment 2: a kind of preparation method of Phalaenopsis tissue culture medium adopts the formula proportion of embodiment 1, prepares induction medium, proliferation medium, strong seedling medium and rooting medium respectively according to following steps and method;

[0040] The preparation method of the induction medium is to weigh thiadiazonium phenylurea, peptone, citric acid, sucrose, and agar in a container, add distilled water, and heat to dissolve; then add 1 / 4MS medium, 6-benzylamino Stir purine and coconut milk evenly; add distilled water to make the volume 1L; then adjust the pH value to 5.8; finally divide into 20 small triangular bottles, seal the bottle mouth with a sealing film, at a high temperature of 123 ° C, a high pressure of 0.12 MPa, Sterilize for 18 minutes, take out while hot, shake well and cool down to prepare induction medium;

[0041] The preparation method of the proliferation medium is to weigh Huabao No. 1, Huabao No. 2, inositol, mul...

Embodiment 3

[0044] Embodiment 3: a kind of Phalaenopsis tissue culture medium, it comprises induction medium, proliferation medium, strong seedling medium and rooting medium;

[0045] The specific formulation of the induction medium is as follows: 1 / 4MS medium 1339mg / L, thiadiazide urea 2mg / L, peptone 1950mg / L, citric acid 30mg / L, sucrose 32000mg / L, agar 6300mg / L, 6 - Benzylaminopurine 2mg / L, coconut milk 120mg / L, distilled water needed to set the volume to 1L;

[0046] The proliferation medium is composed of the following components: Huabao No. 1 420mg / L, Huabao No. 2 1300mg / L, inositol 120mg / L, multidimensional element tablet (29) 320mg / L, peptone 900mg / L, tomato juice 20000mg / L, carrot puree 16000mg / L, apple puree 17000mg / L, banana puree 19000mg / L, coconut milk 80mg / L, rosemary extract 9mg / L, polyvinylpyrrolidone 900mg / L, adenine 1.2mg / L, 0.8mg / L of 6-benzylaminopurine, 1.2mg / L of naphthaleneacetic acid, 19000mg / L of sucrose, 5800mg / L of agar, and the distilled water needed to make u...

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Abstract

The invention discloses phalaenopsis tissue culture media and preparation methods thereof. The phalaenopsis tissue culture media comprises an induction culture medium, a proliferation culture medium,a seedling strengthening culture medium and a rooting culture medium and the preparation methods comprise the preparation methods of the induction culture medium, the proliferation culture medium, theseedling strengthening culture medium and the rooting culture medium . The induction culture medium, the seedling strengthening culture medium and the rooting culture medium are obtained by adding biomass nutrients such as tomato juice, carrot puree, apple puree, banana puree and coconut juice and hormonal nutrients such as adenine, 6-benzylaminopurine and naphthalene acetic acid on the basis ofan MS medium according to nutritional and technical requirements in each culture stage. The phalaenopsis tissue culture media are applied to phalaenopsis tissue culture, have the advantages of less hormone consumption, rapid reproduction, large quantity, tidy seedling emergence, pure varieties, low cost and wide adaptability, and can improve the market competitiveness and promote development of the phalaenopsis industry.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a Phalaenopsis tissue culture medium and a preparation method thereof. Background technique [0002] Phalaenopsis belongs to the Orchidaceae plant and has the reputation of "Queen of Orchids". Its flowers are graceful and elegant, and the flowering period is as long as 3 to 4 months. It has high ornamental value and economic value, and is favored by consumers. Under natural conditions, Phalaenopsis mainly reproduces by ramets and seeds, and the reproductive rate is low. Moreover, due to long-term sexual reproduction, the number of virus-carrying strains increases, which gradually reduces the species and affects growth. Nowadays, tissue culture has become the most commonly used method for asexual reproduction of Phalaenopsis. It can not only maintain the excellent traits of the female parent, avoid the generation of mutant plants, but also adapt to it with the advanta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 杨雪峰随洋王绎陈海军张秀娟
Owner 内蒙古自治区生物技术研究院
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