Method for simply transplanting tissue culture seedlings of eucalyptus urophylla * E. grandis

Abstract

The invention discloses a method for simply transplanting tissue culture seedlings of eucalyptus urophylla * E. grandis. The method comprises the following steps: (1) transferring the tissue culture seedlings taking root well and a culture bottle from a constant-temperature illumination culture box to the outdoor natural environment so as to acclimatize the seedlings; (2) after the seedlings are acclimatized, using a pair of tweezers to take out the tissue culture seedlings in the culture bottle, and using sterile water to rinse agar on root systems of the tissue culture seedlings; (3) placing the rinsed tissue culture seedlings in potassium permanganate solution, soaking for 30 seconds, and using the sterile water to rinse the tissue the culture seedlings after the tissue culture seedlings are taken out; (4) transplanting the rinsed tissue culture seedlings in a cultivation base material, and using the cultivation base material to cover the roots systems of the tissue culture seedlings during the transplanting process; (5) spraying carbendazim on the tissue culture seedlings for one time after transplantation is finished so as to wet 1 / 3-1 / 2 of the upper portion of the cultivation base material; (6) preserving moisture and heat of the tissue culture seedlings until sprouts of the tissue culture seedlings germinate; and (7) transplanting the sprouts of the tissue culture seedlings into the field so as to be cultivated in one week after the sprouts of the tissue culture seedlings germinate. The transplanting survival rate can be over 98%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a simple transplanting method for rooted seedlings of transgenic Eucalyptus urotheca through tissue culture. Background technique [0002] eucalyptus( E. ucalyptus ) is mainly Eucalyptus (M. grtaceae) of Myrtaceae ( E. ucalyptus ) is a general term for plants and is one of the world's three largest fast-growing tree species. Eucalyptus tail ( E. urophylla×E. grandis ) is Eucalyptus urophylla ( E. urophylla ) and Eucalyptus ( E. grandis ) has obvious heterosis, its trunk is straight and uniform, has the advantages of wide adaptability, strong cold resistance, fast growth and high yield, etc. It is an excellent industrial material, and its planting area has accounted for 40% of the eucalyptus plantation area. %, becoming one of the most important fast-growing and high-yielding afforestation tree species in the tropical and subtropical regions of southern my cou...

AI Technical Summary

Problems solved by technology

The long-term high-humidity growth environment affects the morphological structure of transgenic seedling leaves, such as thinning of the cuticle and failure of stomatal regulation, resulting in transgenic seedlings losing more water than absorbing water after transplanting, and making changes to the external environment. The response of transgenic seedlings is correspondingly greater, therefore, it is more difficult for transgenic seedlings to survive transplantation.

[0008] To sum up, there are occasional domestic and foreign literatures on the transplanting methods of tissue cultured seedlings in the process of genetic transformation of Eucalyptus urocarpa, but they are not systematic, and there are no research reports on simple and effective transplanting management methods of rooted seedlings