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Rapid propagation method of Hydrangea macrophylla

A hydrangea, rapid propagation technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult to obtain plants, hydrangea sterile flowers, low reproduction efficiency, etc., to reduce the cost of florists, The effect of fast reproduction speed and large reproduction coefficient

Pending Publication Date: 2020-08-28
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The propagation methods of hydrangea in the original place are mainly cutting, layering, branching, etc. Although these methods are easy to survive, the reproduction efficiency is low and cannot meet the large demand of the market. Propagation by seeds to obtain plants

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Embodiment: the rapid propagation method of the hydrangea in the present embodiment takes the hydrangea stem section as the explant, utilizes MS medium and biological growth regulators such as 6-BA, KT, IBA, GA of different concentrations to induce axillary buds, and the axillary buds proliferate , inducing root formation, seedling hardening, to realize the rapid propagation of Hydrangea, specifically include the following steps:

[0024] (1) Take the annual hydrangea stem section as the explant, and remove the dirt on the surface of the stem section with a soft brush with detergent solution before disinfection. Cut the stem into about 6cm and put it into a beaker, then add two drops of Tween 2.0, cover the mouth of the beaker with gauze, and rinse it with running water for 30 minutes. After draining the water, rinse it with sterile water for 3 times on the ultra-clean workbench, each time for 1 minute, then soak in 75% alcohol for 30 seconds, rinse with sterile water f...

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Abstract

The invention belongs to the technical field of forestry vegetative propagation, and particularly relates to a rapid propagation method of Hydrangea macrophylla. The method comprises the following steps: (1) carrying out sterile disinfection treatment by taking a stem section of the Hydrangea macrophylla as an explant; (2) cutting the Hydrangea macrophylla stem section treated in the step (1) intoHydrangea macrophylla stem sections each with at least one axillary bud, inoculating the Hydrangea macrophylla stem sections into a culture medium, and germinating the axillary buds; (3) when the axillary buds in the step (2) extend to a certain length, cutting off the axillary buds, inoculating the axillary buds into a culture medium, and carrying out proliferation on the axillary buds to obtaina large number of axillary buds; (4) inoculating the axillary buds in the step (3) into a culture medium, and carrying out induction culture to form rooting seedlings; and (5) transplanting tissue culture seedlings in the step (4), namely the rooting seedlings, into a matrix, and carrying out culture to obtain Hydrangea macrophylla aseptic seedlings with robust rhizomes. The method has the advantages of being high in propagation speed and large in propagation coefficient, and is not limited by seasons and propagation materials.

Description

technical field [0001] The invention belongs to the technical field of forestry asexual reproduction, and in particular relates to a rapid propagation method of Hydrangea. Background technique [0002] Hydrangea macrophylla, also known as hydrangea and hydrangea, is a small deciduous shrub of the genus Hydrangea in the saxifrage family. The branchlets are strong, the leaves are large and thick, opposite, and the leaf edges are jagged. The flowers are large and beautiful. The flowers are white at the beginning, and the color of the flowers gradually turns pink or blue with the change of soil pH value. Hydrangea likes warmth and humidity. Not tolerant to drought. [0003] Hydrangea is native to Sichuan and Japan in my country. Hydrangea was cultivated earlier in my country. It was used in gardens in the south of the Yangtze River during the Ming and Qing Dynasties. In recent years, it has been used in landscape greening and gardening design in various parts of the country bec...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 白玉娥王思冉金木兰
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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