New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method

A polyploidization and distant hybridization technology, applied in the fields of botanical equipment and methods, horticultural methods, plant genetic improvement, etc. Excellent material and large shading area

Active Publication Date: 2013-06-26
HUBEI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to propose a method for distant hybridization and polyploidy breeding of new mulberry and mulberry hybrids, which is to establish and obtain the distant hybrids through embryo engineering and tissue culture techniques through reproductive ...

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  • New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method
  • New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method
  • New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method

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Embodiment Construction

[0040] Below with embodiment the present invention is further described:

[0041] a. Obtaining the fruit of distant hybrids

[0042] The excellent mulberry varieties Esang 2 and Husang 32 in the mulberry germplasm resource bank of the Hubei Academy of Agricultural Sciences and the wild mulberry GSf-01 in the Institute of Oil Plants of the Chinese Academy of Agricultural Sciences were selected as parents. The characteristics of the mulberry tree were bagged as the female parent; the male parent mulberry pollen was collected at the early flowering stage, the anthers were taken out and dried in a dry place, and the anthers were wrapped in paper after the anthers cracked and the pollen was scattered, and placed in a dry place Store quicklime in a desiccator. When the mucus appears on the stigma from the first flower to the last flower of the mulberry tree GSf-01, pollinate in 3 times (use a brush to pick the pollen and scatter it on the stigma with mucus), and spray the hybri...

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Abstract

The invention priovidese a new Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method. The method is characterized in that an inductive hybrid fruit callus doubling distant hybridization polyploid hybrid line is established by an embryo engineering and tissue culture technique of distant hybrids through a reproduction engineering kind improvement technology. The method comprises following steps: 1, obtaining distant hybrid fruits; 2, carrying out callus induction of the hybrid fruits; 3, carrying out colchicine treatment of the callus of the hybrid fruits; 4, carrying out recovery culture of treated calluses; 5, differentiating the calluses into bud seedlings; 6, differentiating the bud seedlings into roots; 7, transplanting tissue culture plants; 8, carrying out comparative observation and selection of survival plants; and 9, carrying out asexual propagation of the selected plants to form a stable kind (line). The method solves the problems comprising slow growth,weak adaptability, unclear trunk and bad material quality of present Broussonetia papyrifera, and allows the new polyploid Broussonetia papyrifera mulberry tree hybrid having the advantages of obvious trunk, strong adaptability and good material quality to be cultivated.

Description

technical field [0001] The invention relates to a method for distant hybridization and polyploidy breeding of new mulberry hybrids, and belongs to the technical field of tree breeding in modern agriculture. Background technique [0002] Paper mulberry is a deciduous tree shrub belonging to Moraceae (Moraceae). It has a well-developed root system and extremely developed bast fibers. It has strong adaptability and is extremely resistant to drought, barrenness and humidity. Known as the "Pioneer of Ecological Restoration", it is widely used in the feed industry, but its woodiness is poor and its backbone is not obvious, which limits its application in the timber market and street gardens. As one of the important economic trees in our country, mulberry tree has excellent material and obvious backbone. Its main value lies in silkworm breeding and high-end furniture market materials, but it has poor performance in resistance to diseases and insect pests, and is easy to be eaten by...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 王东鹏陈小芳潘超虎屈海波王维杜明芳宋兆建何玉池蔡得田
Owner HUBEI UNIV
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