Cultivating method suitable for agrobacterium mediated early-maturing rice tissue

A technique of Agrobacterium-mediated tissue culture, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of losing the ability of green seedling differentiation and being unsuitable for Agrobacterium-mediated gene transformation

Inactive Publication Date: 2006-07-05
JIANGSU ACAD OF AGRI SCI +1
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Problems solved by technology

[0005] Technical Problem The purpose of the present invention is to overcome the white, translucent, sticky embryogenic callus cultured in the prior art, which gradually loses the ability of green shoot differentiation during the subculture process, and is not suitable for Agrobacterium-mediated gene transformation defects, providing a method suitable for Agrobacterium-mediated bluegrass tissue culture, the number of light yellow, dry, granular embryogenic callus obtained accounts for more than 40% of the total number of induced callus

Method used

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Embodiment Construction

[0022] 1. Materials and Methods

[0023] 1.1 Test material

[0024] Poa pratensis L.cv.'TotalEclipse' and Poa pratensis L.cv.'Midnight' were provided by Beijing Zhongzhong Grass Industry Co., Ltd.; "Daqingshan" (Poa pratensis L.cv.'Daqingshan') was provided by the Grassland Research Institute of the Inner Mongolia Academy of Animal Husbandry Sciences as the test material.

[0025] 1.2 Callus induction medium

[0026] The basic medium is MS macroelements, microelements, B 5 Vitamins, sucrose 30g / L.

[0027] Additional hormones: 2,4-dichlorophenoxyacetic acid (2,4-D for short, Shanghai Chemical Reagent No. 4 Factory) 2.0 mg / L, 6-benzylaminopurine (BAP for short, Shanghai Xueman Biotechnology Co., Ltd.) mg / L;

[0028] The medium was adjusted to pH 5.8 with sodium hydroxide and hydrochloric acid before autoclaving;

[0029] The amount of agar strips (Chengfeng brand, Quangang Chemical Factory, Quanzhou City, Fujian Province) was used to set up 3 groups of comparative tests: ...

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Abstract

A tissue culture method of early-ripe rice by use of agrobacterium mediation features that in the culture medium of callus, the consumption of agar as the solidifying agent is increased by one time to increase the resultant calli by 40%, which can be used as the receptor of transgenosis. With said method, the pest and disease resistant genetically engineered plants are obtained.

Description

1. Technical field [0001] The invention discloses a bluegrass tissue culture method suitable for Agrobacterium-mediated method, belongs to plant tissue culture method, and is specially used for the cultivation of acceptor material of Agrobacterium-mediated gene transformation. 2. Technical background [0002] Bluegrass (Poa pratensis) is a cool-season turfgrass and one of the important grass species in temperate regions. Because of its beautiful color, strong cold resistance, shade tolerance, and pruning resistance, it is often used as one of the lawn grass species in northern my country. Bluegrass grass also has its own disadvantages, such as being susceptible to pests and diseases, and not tolerant to heat. [0003] The maturation of plant transgenic technology has opened up a new way of molecular biology breeding for the improvement of turfgrass. The Agrobacterium-mediated method has the characteristics of simple operation, high transformation efficiency, and good genet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 佘建明张保龙倪万潮何晓兰陈志一朱琼
Owner JIANGSU ACAD OF AGRI SCI
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