New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method

Abstract

The invention priovidese a new Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method. The method is characterized in that an inductive hybrid fruit callus doubling distant hybridization polyploid hybrid line is established by an embryo engineering and tissue culture technique of distant hybrids through a reproduction engineering kind improvement technology. The method comprises following steps: 1, obtaining distant hybrid fruits; 2, carrying out callus induction of the hybrid fruits; 3, carrying out colchicine treatment of the callus of the hybrid fruits; 4, carrying out recovery culture of treated calluses; 5, differentiating the calluses into bud seedlings; 6, differentiating the bud seedlings into roots; 7, transplanting tissue culture plants; 8, carrying out comparative observation and selection of survival plants; and 9, carrying out asexual propagation of the selected plants to form a stable kind (line). The method solves the problems comprising slow growth,weak adaptability, unclear trunk and bad material quality of present Broussonetia papyrifera, and allows the new polyploid Broussonetia papyrifera mulberry tree hybrid having the advantages of obvious trunk, strong adaptability and good material quality to be cultivated.

Description

technical field [0001] The invention relates to a method for distant hybridization and polyploidy breeding of new mulberry hybrids, and belongs to the technical field of tree breeding in modern agriculture. Background technique [0002] Paper mulberry is a deciduous tree shrub belonging to Moraceae (Moraceae). It has a well-developed root system and extremely developed bast fibers. It has strong adaptability and is extremely resistant to drought, barrenness and humidity. Known as the "Pioneer of Ecological Restoration", it is widely used in the feed industry, but its woodiness is poor and its backbone is not obvious, which limits its application in the timber market and street gardens. As one of the important economic trees in our country, mulberry tree has excellent material and obvious backbone. Its main value lies in silkworm breeding and high-end furniture market materials, but it has poor performance in resistance to diseases and insect pests, and is easy to be eaten by...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method for distant hybridization and polyploidy breeding of new mulberry and mulberry hybrids, which is to establish and obtain the distant hybrids through embryo engineering and tissue culture techniques through reproductive engineering variety improvement technology. Hybrid fruit and induced hybrid fruit callus are doubled to form distant hybrid polyploid hybrid lines, which are used to solve the problems of unknown trunks of existing mulberry trees, poor material quality and poor adaptability of mulberry trees

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