Method for obtaining conifer embryoid through liquid culture and its culture medium

A technology of liquid medium and embryoid body is applied in the field of cultivation of plant explants, and can solve the problems of vitrification, hypocotyl failure, abnormal cotyledon development, etc.

Inactive Publication Date: 2007-12-26
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For coniferous tree species, commonly used in vitro media include MS, DCR, SH, LP, GD, etc. The existing problems mainly include: the early proembryo formation state is not good, the quality of embryogenic callus is not high, and the early proembryo Incomplete embryo development and abnormal development of cotyledon, vitrification problem, hypocotyl non-elongation, somatic embryo not rooting, etc.

Method used

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  • Method for obtaining conifer embryoid through liquid culture and its culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0178] 1. Experimental materials and methods

[0179] 1. Experimental materials

[0180] Immature zygotic embryos of Larix principis, Japanese larch, Larix olgensis, Japan × North China hybrid larch, and Japan × Landrace hybrid larch.

[0181] Larix kaempferi were selected from the 30-year-old seed garden of the Dagujia Forest Farm in Qingyuan County, Liaoning Province, including Riyong 8, Riyong 85, Rifu 27, and Ricao 103 clones; Larix olgensis was collected from the Hada Forest Farm in Fushun City, Liaoning Province The 30-year-old white larch seed orchard includes the Chang 3, Chang 14, Chang 11, Chang 4C, and Chang 33 clones; Larix principis is collected from the Qiuqiangou Forest Farm of Guancheng Forestry Bureau in Shanxi Province. 6. Hua 7 and other clones. The hybridization techniques of the Japanese, Landrace, and Larix principis-rupprechtii combinations are carried out using open controlled pollination.

[0182] 2. Experimental method

[0183] 1) Inoculation, induction c...

Embodiment 2

[0199] The culture was carried out in the same manner as in Example 1, and the medium used was as shown in Table 3. The somatic embryo culture of coniferous species was carried out, and the same results as in Example 1 were obtained.

[0200] The difference is that the proliferation culture is carried out in a fermenter, and the inoculation amount is 0.5-1% by weight to volume, that is, 0.5-1 g of fresh, free embryonic cell lines are inoculated in 100 ml of liquid medium. Cultivation is carried out at 23±5°C and dark culture conditions.

[0201] Table 3 Medium formula (mg / L)

[0202] Compound

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Abstract

The invention embryoid of needle leaf trees through liquid cultivation and culture media, wherein the media comprise induction culture media, propagation culture media and maturation culture media, the method for obtaining the embryoid of needle leaf trees comprises the steps of induction culture, propagation culture and maturation culture.

Description

Technical field [0001] The invention relates to a method for culturing plant explants, in particular to a method for obtaining coniferous species embryoid bodies by liquid culture and its culture medium. Background technique [0002] Plant somatic embryogenesis (somatic embryogenesis) refers to the morphogenesis of diploid or haploid somatic cells under certain conditions, without the fusion of sex cells, but through a similar approach to zygotic embryogenesis; Somatic embryogenesis forms a structure similar to zygotic embryos called somatic embryos (somaticembryo); somatic embryogenesis is developed from a type of tissue called embryogenic cells, which is the technical system of somatic embryogenesis The key to whether it can be established. With the rapid development of modern biotechnology, somatic embryogenesis has become an important way for plant regeneration in conifer cell engineering. It can be used to study the origin, regulation and development of clonal cells, which i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 齐力旺张守攻韩素英王建华
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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