A method for inducing tetraploid silage corn

A technology for silage corn and tetraploid, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problem of limiting the growth and development speed of polyploid meristematic cells and their daughter cells, and the probability of corn ears being induced Low, low frequency of tetraploid corn, etc., to achieve the effect of improving seed activity, improving permeability, and improving induction efficiency

Inactive Publication Date: 2018-11-09
辽宁省农业科学院玉米研究所 +2
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Problems solved by technology

[0004] (1) The structure of the seed bud is relatively complicated. The growth point of the seed bud is also wrapped with multiple tissues such as the coleoptile, incomplete leaf, first true leaf, and second true leaf. The doubling solution needs to pass through multiple tissues In order to penetrate into the growth point of the seed bud and induce polyploidization of its cells, it is more difficult for the doubling solution to penetrate into the growth point of the seed bud
[0005] (2) The developmental progress of growth point cells with different chromosome ploidy in the seed bud is inconsistent, that is, diploid meristem cells have more advantages in growth and development than polyploid meristem cells, so that diploid mer

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  • A method for inducing tetraploid silage corn
  • A method for inducing tetraploid silage corn
  • A method for inducing tetraploid silage corn

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[0021] (1) Seed soaking: Spread the seeds of silage corn into a glass petri dish, add clean water until the seeds have just been submerged, and cultivate in an incubator at 20-25°C for 24 hours. Take out the petri dish, put it in a refrigerator at 4°C for 12h, move it to an incubator at 20-25°C for 2-3h, repeat this step.

[0022] (2) Treatment during the germination period of the double solution: Pour out the water in the petri dish, add the double solution, and leave the seeds in the refrigerator at 4°C for 12 hours.

[0023] (3) Infrared irradiation: Rinse the seeds with clear water, irradiate each dish with an infrared lamp (50Hz) for 20-30s, add clear water, and incubate at 20-25℃ for 24h.

[0024] (4) Double solution seedling stage treatment: Plant the seeds in the nursery pots and manage them normally. When the seedlings grow to 3-4 leaves, use a 1ml syringe to inject 0.5ml of the double solution into the growth point of the seedling stem until the solution is from the leaves....

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Abstract

The invention discloses an induction method of tetraploid silage corn by use of colchicines. The method is as follows: diploid corn is used as an induction material, and the induction process mainly includes the following steps: seed soaking; doubling solution germination period treatment; infrared irradiation; and doubling solution seedling stage injection. The method can significantly increase the induction frequency of the tetraploid silage corn, and compared with the diploid corn, the tetraploid silage corn obtained by doubling has the advantages of large plant type, wide and fleshy leaves, thick stems and roots and the like, and is excellent silage. Therefore, the application of the induction method can lay a solid foundation for the research and development of the tetraploid silage corn.

Description

1. Technical field [0001] The invention relates to a tetraploid induction method for silage corn, belonging to the technical field of plant breeding 2. Background technology [0002] Most varieties of silage corn have high nutritional value and high biological yield per unit area. They have the characteristics of fragrant smell, soft and juicy, good palatability, more nutrients in raw materials and less loss. They are especially suitable for dairy cows and beef cattle all year round. Excellent feed in winter and spring. Under the situation of "straw animal husbandry", in order to meet the forage demand of the rapid development of dairy cattle and beef cattle breeding industry and relieve the pressure on pastures, silage corn has great potential in production and utilization, and it is also a solution to the contradiction between supply and demand of grain and feed in my country. A better way to realize the effective supply of grain and feed. [0003] Plant polyploidy exists...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 王延波赵念力马骏弓雪姜敏齐欣刘欣芳刘祥久王金君孟庆国李泉木李明李刚贾卓张秀梅
Owner 辽宁省农业科学院玉米研究所
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