A method for inducing tetraploid silage corn

Abstract

The invention discloses an induction method of tetraploid silage corn by use of colchicines. The method is as follows: diploid corn is used as an induction material, and the induction process mainly includes the following steps: seed soaking; doubling solution germination period treatment; infrared irradiation; and doubling solution seedling stage injection. The method can significantly increase the induction frequency of the tetraploid silage corn, and compared with the diploid corn, the tetraploid silage corn obtained by doubling has the advantages of large plant type, wide and fleshy leaves, thick stems and roots and the like, and is excellent silage. Therefore, the application of the induction method can lay a solid foundation for the research and development of the tetraploid silage corn.

Description

1. Technical field [0001] The invention relates to a tetraploid induction method for silage corn, belonging to the technical field of plant breeding 2. Background technology [0002] Most varieties of silage corn have high nutritional value and high biological yield per unit area. They have the characteristics of fragrant smell, soft and juicy, good palatability, more nutrients in raw materials and less loss. They are especially suitable for dairy cows and beef cattle all year round. Excellent feed in winter and spring. Under the situation of "straw animal husbandry", in order to meet the forage demand of the rapid development of dairy cattle and beef cattle breeding industry and relieve the pressure on pastures, silage corn has great potential in production and utilization, and it is also a solution to the contradiction between supply and demand of grain and feed in my country. A better way to realize the effective supply of grain and feed. [0003] Plant polyploidy exists...

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Problems solved by technology

[0004] (1) The structure of the seed bud is relatively complicated. The growth point of the seed bud is also wrapped with multiple tissues such as the coleoptile, incomplete leaf, first true leaf, and second true leaf. The doubling solution needs to pass through multiple tissues In order to penetrate into the growth point of the seed bud and induce polyploidization of its cells, it is more difficult for the doubling solution to penetrate into the growth point of the seed bud

[0005] (2) The developmental progress of growth point cells with different chromosome ploidy in the seed bud is inconsistent, that is, diploid meristem cells have more advantages in growth and development than polyploid meristem cells, so that diploid meristem cells A large number of daughter cells produced by the cells limit the growth and development speed of polyploid meristematic cells and their daughter cells, and finally only a large number of daughter cells produced by most diploid meristem cells can complete the morphological construction of the plant. Therefore, the corn ears of contemporary plants have a low chance of being induced

[0006] The above two reasons lead to the low frequency of obtaining autotetraploid maize by using the traditional seed bud induction method. Therefore, exploring a doubling technology to increase the induction rate of tetraploid silage maize will lay a solid foundation for the development and application of tetraploid silage maize. Base

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