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Method for transplanting tissue culture seedlings of dendrobium officinale

A technology of dendrobium candidum and tissue culture seedlings, which is applied in the field of transplantation of dendrobium candidium tissue culture seedlings, can solve the problems of large-scale disease, poor stress resistance, and weak growth of dendrobium candidium seedlings, and achieve less disease, improved plant disease resistance, Effect of Improving Transplant Survival Rate

Inactive Publication Date: 2014-04-23
GUANGXI SENXIANYUAN BIOLOGICAL SCI & TECH
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  • Abstract
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Problems solved by technology

[0004] The disadvantages of the prior art are: Dendrobium officinale seedlings grow weakly, and after transplanting for half a year, the average height of the seedlings is only 6cm, and the average diameter is 0.3cm thick; the stress resistance is poor, and the disease often occurs in a large area; the transplant survival rate is low, only 10-60%

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  • Method for transplanting tissue culture seedlings of dendrobium officinale

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Embodiment 1

[0028] (1) Tissue-cultured seedlings out of the bottle: Take out the tissue-cultured seedlings of Dendrobium officinale from the glass bottle with long-handled tweezers, and place them in a clean plastic basin;

[0029] (2) Rinsing: put the tissue cultured seedlings in clean water, and rinse the culture medium remaining in the roots;

[0030] (3) Disinfection: Put the washed bottle seedlings in a plastic pot, soak them with 1000 times thiophanate-methyl or carbendazim for 10 minutes, then remove them and put them in a cool place to dry until the roots turn white, waiting for transplantation;

[0031] (4) Transplantation: spread the fully fermented broken bark or sawdust on the seedbed, the thickness of the substrate is 10-18cm, first drench it with clean water, and then sterilize it with 800 times of fungicide. After the substrate is drained, put The sterilized bottle seedlings are transplanted on the substrate, generally 2 to 3 buds form a small clump, and the planting size b...

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Abstract

The invention discloses a method for transplanting tissue culture seedlings of dendrobium officinale. The method includes the steps that the tissue culture seedlings of dendrobium officinale are taken out, rinsed, disinfected and then transplanted to a soiless substrate, three to four days after the tissue culture seedlings are field-planted, a vitality element and a nutritional agent are sprayed to leaf surfaces in turn within a month respectively for eight to ten times, the vitality element or the nutritional agent is sprayed every day, and then conventional plantation management is executed. The method for transplanting the tissue culture seedlings of dendrobium officinale has the advantages that the vitality element and the nutritional agent are sprayed to the leaf surfaces of the tissue culture seedlings in turn, vitality of the seedlings is enhanced, the growth vigor of the seedlings is vigorous and robust, leaf color is strong green and glossy, the average height of the seedlings is 15cm after the seedlings are transplanted for half a year, and the average thickness of rootstocks is 0.5cm; disease resistance of the seedlings is improved, and the seedlings are barely diseased; the transplanting survival rate is greatly increased and can reach more than 98%.

Description

technical field [0001] The invention relates to the field of agricultural planting, in particular to a method for transplanting tissue-cultured seedlings of dendrobium officinale. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: black knot grass, Yunnan iron bark. Belonging to the order of Micros, it is a perennial epiphytic herbaceous plant of Orchidaceae. Stem erect, cylindrical, 9-35 cm long, 2-4 mm thick, sepals and petals yellow-green, nearly similar, oblong-lanceolate, about 1.8 cm long, 4-5 mm wide, flowering period March-June. Mainly distributed in Anhui, Zhejiang, Fujian and other places in China. Its stem can be used as medicine, which belongs to the yin-tonifying medicine in tonic medicine, which can benefit the stomach and promote body fluid, nourish yin and clear away heat. [0003] At present, the transplantation of Dendrobium candidum tissue culture seedlings all adopts the soilless ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06
Inventor 李培泰李道强
Owner GUANGXI SENXIANYUAN BIOLOGICAL SCI & TECH
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