Efficient cleft grafting method of dicotyledon seedlings and young branches

A technology of dicotyledonous plants and young shoots, applied in the field of agriculture, can solve the problems of inconvenient rootstock leaf scion nutrition, difficulty of scion insertion and fixation, and pending establishment, etc., to achieve the effect of promoting rapid healing, wide application range, and fast grafting speed

Active Publication Date: 2017-12-26
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) The conventional "split grafting method" sticks to the cotyledon petiole to remove the head and remove the heart, the incision is perpendicular to the cotyledon petiole, the cotyledon will droop to both sides due to gravity, and it is difficult to insert and fix the scion
There are also some researchers who leave a section of epicotyl at the upper end of the cotyle

Method used

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  • Efficient cleft grafting method of dicotyledon seedlings and young branches
  • Efficient cleft grafting method of dicotyledon seedlings and young branches
  • Efficient cleft grafting method of dicotyledon seedlings and young branches

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1. Grafting transgenic regenerated cotton plants on sea-island cotton with 1 to 2 true leaves

[0034] At the end of autumn, winter and spring, the temperature of the greenhouse is more suitable. Graft directly in the greenhouse, and the optimum temperature of the greenhouse is controlled at 28°C±1°C; in summer and early autumn, the temperature is higher, and the grafting is carried out in 50% of the awning.

[0035] Rootstock: Potted cotton cultivar Sea Island cotton seedlings in 20 pots, 5 plants in each pot, a total of 100 plants, after the cotyledons are fully expanded, transplanted into plastic pots with a diameter of about 20 cm, one plant in each pot, as a rootstock.

[0036] Scion: Aseptic transgenic cotton seedlings were cultured as scion.

[0037] Step1: The day before grafting, water the sea-island cotton (cotton seedlings with 1 to 2 true leaves and 2 intact cotyledons) planted in flower pots, keep only 2 cotyledons of the rootstock, and paste the...

Embodiment 2

[0044] Embodiment 2. Grafting transgenic regenerated cotton plants on sea-island cotton with 4 to 6 true leaves

[0045] Rootstock: Potted cotton cultivar Sea Island cotton seedlings in 20 pots, 5 plants in each pot, a total of 100 plants, after the cotyledons are fully expanded, transplanted into plastic pots with a diameter of about 20 cm, one plant in each pot, as a rootstock.

[0046] Scion: Aseptic transgenic cotton seedlings in culture medium are used as scion.

[0047]Step1: The day before grafting, water the sea-island cotton (cotton seedlings with 4 to 6 true leaves) planted in the flower pot with sufficient water, keep 2 cotyledons and 1 to 2 true leaves of the rootstock, and use a sharp The blade cuts off the upper stem and leaf, and then starts from the 5mm lower edge of the petiole of the true leaf, and cuts the stem and the lower edge of the petiole longitudinally from the middle along the central axis of the stem. The incision is neat and smooth, and the incisio...

Embodiment 3

[0054] Embodiment 3, grafting cucumber seedlings on the black-seeded pumpkin with 1 to 2 true leaves

[0055] In early spring, sow 100 pots of black-seeded pumpkin and 100 cucumbers in nutrient bowls, 2 to 3 plants in each pot. When the cotyledons are fully unfolded, leave 1 stronger plant in each pot.

[0056] Rootstock: When the pumpkin seedling grows 1-2 true leaves, and the first true leaf is about 1 cm long, it can be used as a rootstock.

[0057] Scion: 10 days after sowing, the cotyledons of cucumber seedlings will flatten out, and a true leaf and growth point will grow, which can be used as a scion.

[0058] Step1: On the day before grafting, water the pumpkin seedlings (seedlings with 1 to 2 true leaves and 2 intact cotyledons) planted in the nutrient pot with sufficient water, keep only 2 cotyledons of the rootstock, and paste them with a sharp blade. Cut off the stem and leaves above the cotyledon petiole, and then start from the lower edge of the cotyledon petiole...

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Abstract

The invention discloses an efficient cleft grafting method of dicotyledon seedlings and young branches. Rootstocks with 1-2 true leaves (only 2 cotyledons are reserved) or rootstocks with 4-6 true leaves (2 cotyledons and 1-2 true leaves are reserved) are adopted, and stems and the lower edges of petioles are vertically split in the middle along the central axes of the stems from the lower edges of the petioles of the cotyledons and the true leaves; the incisions of scions are cut into a V shape; the stems of the scions are perpendicularly inserted into the incisions of the rootstocks. The incisions of the rootstocks run through the lower sections of the petioles and the central axes of the stems at the same time, so that fixation of the scions is convenient, the scions are closely combined with the rootstocks naturally, nutrient substances produced by photosynthesis of the leaves are transported into the scions nearby through the petioles, and rapid healing of connectors is promoted. In combination with water retention and heat preservation in a recovery period, the survival rate of grafting can be stabilized at 98% or above, and the survival rate of grafting can reach 100% in an appropriate cooperation period of the rootstocks and the scions; according to the method, operation is easy and convenient, grafting is fast, and the survival rate is high.

Description

technical field [0001] The invention relates to a splitting method for plant seedlings and young shoots, in particular to a high-efficiency splitting method for dicotyledonous plant seedlings and young shoots, and belongs to the field of agricultural technology. Background technique [0002] Genetic engineering and cell engineering are important methods for genetic improvement of cotton today. Cotton tissue culture regeneration plants are the basis for the application of transgenic technology in cotton genetic improvement. However, most of the test-tube seedlings of transgenic regeneration cotton plants obtained through somatic embryogenesis have stunted root systems. It manifests as rootless seedlings, brown rooted seedlings, and deformed seedlings (such as clustered seedlings), etc., and the regenerated plants with normal root systems are transplanted into soil or vermiculite, the survival rate is extremely low (usually only 10-20%), and the growth is slow, which greatly li...

Claims

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Application Information

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IPC IPC(8): A01G1/06A01H4/00
CPCA01G2/30A01H4/008
Inventor 秦丽霞吴慎杰张换样李静李盛焦改丽竹梦婕
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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