Bionic microfluidic cell culture chip

A cell culture and microfluidic technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve problems such as large changes in speed and pressure, adverse effects of cell culture microenvironment, etc. Increase the authenticity and balance the effect of the micro-environment

Inactive Publication Date: 2016-02-24
DALIAN UNIV OF TECH
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Problems solved by technology

However, the fluid enters the cell culture tank through the perfusion channel only after the flow channel is filled. The fluid inside the perfusion channel has a high velocity and pressure, and it is easy to generate jets at the interface between the channel and the culture tank, resulting in the velocity and pressure inside the cell culture tank. Large pressure changes can adversely affect the microenvironment of the cell culture

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  • Bionic microfluidic cell culture chip
  • Bionic microfluidic cell culture chip
  • Bionic microfluidic cell culture chip

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Embodiment Construction

[0025] A specific implementation process of the present invention will be further described below in conjunction with the accompanying drawings. The specific implementation described here is only used to explain the present invention, and is not intended to limit the present invention.

[0026] Such as figure 2 As shown, the bionic microfluidic cell culture chip has a three-layer structure, which are: the covering layer of the cell injection hole ( figure 1 in) (the first layer), the cover sheet layer without microstructure ( figure 1 ) (second layer) and fluid channel layer ( figure 1 Middle) (the third layer), wherein the second cover layer without microstructure and the third fluid channel layer constitute the main structure of the biomimetic microfluidic cell culture chip through the oxygen plasma bonding process.

[0027] The invention provides a novel bionic microfluidic cell culture chip, which ensures the stability of fluid velocity, pressure distribution and soluti...

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Abstract

The invention provides a bionic microfluidic cell culture chip. The bionic microfluidic cell culture chip is based on the moisture conveying characteristic in a dicotyledon reticular vein, and comprises a main channel (6) and a cell culture cavity (9) located in the main channel. The cell culture cavity (9) is isolated from the main channel through a micro-column array (8) with the shape consistent with the shape of the culture cavity. The width of slits of the micro-column array (8) is determined by the size of cultured cells. Cell culture fluid is conveyed to the cell culture cavity through the slits of the micro-column array (8) in a convection diffusion mode, direct impact of the cell culture fluid on the surfaces of cells is avoided, influences of the cell culture fluid on cells are reduced, a stable microenvironment is provided for cell culture in vitro, and cell research accuracy is ensured.

Description

technical field [0001] The invention relates to a microfluidic cell culture chip based on a bionic principle. technical background [0002] In vitro cell culture has strict requirements on the environment. It is necessary to control the microenvironment of the cells as much as possible, but also to keep the experiment simple, and to quickly and conveniently repeat the experimental conditions. Microfluidic technology enables precise control of the microenvironment required for cell growth while reducing the scale of experiments. Therefore, the use of microfluidic chips for cell culture is currently the preferred method for cell research. [0003] For microfluidic cell culture devices, the fluid is mainly responsible for the supply of culture fluid and the removal of waste fluid, but the fluid flow will generate a certain shear stress, which often acts on part of the surface of the cell, rather than uniform distribution on the whole cell. Therefore, the action of shear stre...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/16C12M25/06
Inventor 李经民马亚会刘冲魏娟刘海侠
Owner DALIAN UNIV OF TECH
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