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Aseptic germination method of Crassulaceae plant seeds

A plant seed, the technology of Crassulaceae, applied in the field of Crassulaceae plants, can solve the problems of low survival rate of seedlings, difficulty in seed collection, low germination rate, etc., and achieve the effect of shortening germination time, good development and increased germination rate

Inactive Publication Date: 2015-01-14
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruits of sedum plants are small, the seeds are difficult to collect, and the price is expensive. The traditional soil sowing method has a low germination rate and a low survival rate of seedlings. It is difficult to obtain a certain amount of breeding materials with excellent traits in a short period of time. The sowing method of sedum plants that is not restricted by seasons has become the need of the situation

Method used

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  • Aseptic germination method of Crassulaceae plant seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Medium preparation

[0066] Prepare plant MS solid medium, add GA 3 (Gibberellin, the final concentration is 5mg / L) and BA (benzylaminoadenine, the final concentration is 0.5mg / L), adjust the pH value to 5.8, high temperature and high pressure sterilization, pour into the 9cm plate for subsequent use.

[0067] (2) Seed disinfection

[0068] Select the seeds (purchased from the market) of three kinds of Crassulaceae plants Guanghangong, Red Claw and Sweet Clover, and remove impurities from the seeds.

[0069] Put the seeds into a 1.5ml EP tube, add 0.01%-0.05% trace potassium permanganate solution (lavender) to soak for 1 hour, rinse and transfer to a 1.5ml EP tube and add 10% sodium hypochlorite solution for 8 minutes. Mix the seeds with the solution for 3-5 times with a vortex oscillator, suck out the solution after placing it in the ultra-clean bench, and rinse with sterile double distilled water for 3-5 times for about 5 minutes.

[0070] (3) Sowing

[0071] ...

Embodiment 2

[0080] The steps of the present embodiment are basically the same as in Example 1, the difference is that with Sedum aurea (ex El Hierro), Greenovia aurea (ex Gran Canaria), Greenovia aurea (ex Tenerife), black lotus (Echeveria eurychlamys, Peru), East cloud (Echeveria agavoides) Echeveria olivacea, Guienagati seeds, fairy cup (Dudleya lanceolata) and moon shadow (Echeveria halbingeri, San Joaquin-v sanchez-mejoradae, Ahualulco) and other plant seeds were aseptically germinated, and the results showed that in Under the same conditions, the aseptic germination rate of these representative ornamental plants of Crassulaceae also reached more than 99%. After rooting and transplanting, small plants were obtained. The whole process of obtaining commercialized large seedlings only needs about 8-9 moon.

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Abstract

The invention discloses an aseptic germination method of Crassulaceae plant seeds. The method comprises the steps of aseptic germination of sowed seeds, and rooting culture. The method allows the Crassulaceae plant seeds to be aseptically germinated and cultured to form plants, realizes the mass efficient germination of the Crassulaceae plant seeds within a short time, and allows roots to be generated, rooted seedlings to be transplanted to soil, the transplanted seedlings to rapidly grow and commercial plants to be obtained within a short period.

Description

technical field [0001] The invention relates to Crassulaceae plants, in particular to a method for aseptic germination of Crassulaceae plant seeds. Background technique [0002] Crassulaceae plants are a class of succulents that are widely distributed and have high ornamental and medicinal value. Sedum family plants are dicotyledonous plants, there are about 1600 species, distributed all over the world, herb or subshrub, usually succulent. Leaves alternate, opposite or whorled, solitary, mostly sessile, entire, serrated, lobed or pinnatifid, without stipules. The fruit is a follicle, membranous or hairy, often surrounded by the remaining membranous petals, dehiscing; the seeds are small, disc-shaped, often with fleshy endosperm. There are about 25 genera and more than 1,000 species in the world, native to Africa, the Americas and the temperate regions of Europe. [0003] There are about 240 species of 10 genera of Crassulaceae plants in China, and many others are introduc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马俐朱木兰李胜
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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