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Crassulaceae root growth-promoting Bacillus aryabhattai and application thereof

A technology of Bacillus aeruginosa, rhizosphere growth promotion, applied in the application, bacteria, biocide and other directions, to achieve the effect of promoting growth and strong pertinence

Active Publication Date: 2016-07-13
SHANDONG FOREST SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the screening and application of PGPR strains of Crassulaceae plants.

Method used

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  • Crassulaceae root growth-promoting Bacillus aryabhattai and application thereof
  • Crassulaceae root growth-promoting Bacillus aryabhattai and application thereof
  • Crassulaceae root growth-promoting Bacillus aryabhattai and application thereof

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Experimental program
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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0020] 1. Screening process

[0021] Three rhizosphere soil samples of sedum succulents from the production base of sedum succulents in Huanglou Town, Qingzhou City, Shandong Province were collected. Bacterial isolates were isolated from rhizosphere soil samples by serial dilution method. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use.

[0022] to R containing L-tryptophan 2 Bacteria were inoculated in liquid culture medium A, cultured on a shaker at 28° C., 180 rpm, for 4 days. Take 50 microliters of bacterial suspension and drop it on a white ceramic plate, and add 50 microliters of Salkowski colorimetric solution (50ml35%HCLO 4 +1ml0.5mol / LFeCl 3 ). Add 50mg / LIAA c...

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Abstract

The invention discloses crassulaceae root growth-promoting Bacillus aryabhattai JIA2 which has been preserved in the China General Microbiological Culture Collection Center in January 7, 2016, and the preservation No. is CGMCC No. 11967. The crassulaceae root growth-promoting bacillus aryabhattai has very good specificity, can be successfully colonized in root soil of crassulaceae succulent plants, and can remarkably promote growth of the crassulaceae succulent plants.

Description

technical field [0001] The invention relates to a sedum rhizosphere growth-promoting bacillus arborii and application thereof. Background technique [0002] Crassulaceae plants (Crassulaceae) are perennial succulent herbs with a small and exquisite appearance, plump and juicy plants, unique shapes and various appearances, and are deeply loved by consumers. Sedum succulents play an important role in landscaping and interior decoration, and are also the main material for green roofs. With the rapid development of the succulent plant industry, improving the quality of cultivation, shortening the cultivation period, and reducing the cultivation cost are urgent needs for the production of succulents of the Crassulaceae family. [0003] The beneficial effect of rhizosphere microorganisms on plants is mainly manifested in the improvement of plant nutrient absorption and the promotion of plant growth. The use of biological inoculation technology to improve the rhizosphere microbial...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 马海林刘方春杜秉海马丙尧丁延芹李丽杜振宇姚良同单连娟
Owner SHANDONG FOREST SCI RES INST
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