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Tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants

A technology for succulent plant and tissue culture, which is applied in the field of plant cultivation, can solve the problems of difficult maintenance, difficult pollination, and easy death in the seedling stage, and achieves the effects of improving the survival rate of reproduction and solving the problem of a single reproduction method.

Pending Publication Date: 2022-02-11
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problems of low reproductive rate, difficult pollination, difficult maintenance at the seedling stage, and easy death caused by seed propagation of the existing succulent plants of the genus Pseudomonas genus Sedum, the present invention provides a Sedum sedum Method for Tissue Culture Propagation of Succulents of the Genus Dongyun System

Method used

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  • Tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants
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  • Tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants

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Embodiment 1

[0023] A method for tissue culture and propagation of succulents of the sedum family Pseudomonas genus Dongyun system, is characterized in that it comprises the following steps:

[0024] 1) Disinfection treatment of explants: the selected 'HBG' flower arrows were used as explants, moved to the ultra-clean workbench, soaked in alcohol solution with a mass concentration of 75% for 30s, and then washed with sterile water for 3 Once again, sterilize with 0.08% mercuric acid solution mixed with Tween for 5 minutes. The amount of Tween is to add 2 drops of Tween per 1000ml of mercuric solution, and finally rinse it with sterile water for 4 times and clean it with sterile filter paper. Blot up the water, cut off the injured part during incision disinfection, the contamination rate is 20.5%, the survival rate reaches more than 50%, and sterile explants are obtained;

[0025] 2) Induction culture: Inoculate the sterile explants from step 1) in the following induction medium: MS+0.5mg / L...

Embodiment 2

[0030] A method for tissue culture and propagation of succulents of the sedum family Pseudomonas genus Dongyun system, is characterized in that it comprises the following steps:

[0031] 1) Disinfection treatment of explants: take the selected 'American double-headed ebony' flower arrow as explants, move them to the ultra-clean workbench, soak them in alcohol solution with a mass concentration of 75% for 30 seconds, and then wash them with sterile water 5 times, and then sterilized with 0.12% mercuric chloride solution mixed with Tween for 8 minutes. The amount of Tween was to add 3 drops of Tween per 1000ml of mercuric liter solution, and finally rinsed with sterile water 5 times and washed with sterile water. The filter paper absorbs the moisture, cuts off the injured part during incision disinfection, and obtains sterile explants, with a contamination rate of 10.2% and a survival rate of over 30%;

[0032] 2) Induction culture: inoculate the sterile explants from step 1) in...

Embodiment 3

[0037] A method for tissue culture and propagation of succulents of the sedum family Pseudomonas genus Dongyun system, is characterized in that it comprises the following steps:

[0038] 1) Disinfection of explants: take the selected 'Armstrong' lateral bud leaves as explants, move them to the ultra-clean workbench, soak them in alcohol solution with a mass concentration of 75% for 30s, and then wash them with sterile water Wash 4 times, then sterilize with 0.10% mercuric chloride solution mixed with Tween for 6 minutes. Bacteria filter paper was used to absorb the water, and the injured part during incision disinfection was cut off to obtain sterile explants with a contamination rate of 5.0% and a survival rate of over 20%;

[0039] 2) Induction culture: inoculate the sterile explants from step 1) in the following induction medium: MS+0.7mg / L 6-BA+0.5mg / L NAA+25g / L sugar+7g / L agar with a pH value of 5.9, after 38 days of induction culture at 25±2°C, light intensity of 2500lx...

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Abstract

The invention provides a tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants. The tissue culture and propagation method is characterized by comprising the following steps: 1) disinfection treatment of explants, 2) induction culture, 3) subculture, 4) rooting culture, 5) seedling hardening and transplanting to obtain complete plants. According to the method, the propagation survival rate of crassulaceae echeveria agavoides succulent plants can be increased; compared with a traditional propagation mode, the established tissue culture regeneration system is not limited by seasons, materials, and environments, the plants can be produced all the year round by manually controlling the environment needed by the plants, so that the problems that the echeveria agavoides succulent plants are single in propagation mode, low in speed, rare in market and the like are solved, and technical support is provided for industrialization and marketization of seedlings for the echeveria agavoides succulent plants.

Description

Technical field [0001] The invention relates to a culture and propagation method, in particular to a tissue culture and propagation method of succulent plants of the genus Echeveria of the Sedum family, belonging to the technical field of plant cultivation. Background technique [0002] Echeveria in the Sedum family is a succulent plant. According to its leaves and plant types, it can be divided into Yueying series, Jilian series and Dongyun series. Compared with other Echeveria plants, the Echeveria genus ( Echeveria agavoides ) The problems with succulents are as follows: first, there are fewer branches; second, seedlings that are 2-3 years old are basically impossible to divide; third, the survival rate of leaf cuttings is extremely low. At present, the more popular Dongyun varieties on the market are generally propagated by seeds. However, seeds are not easy to survive, and the flowers are very small, making pollination difficult. Moreover, the maintenance of Dongyun s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 曹桦李涵叶涨涨陆琳李绅崇苗振
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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