Herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa and application thereof

A Pseudomonas aeruginosa and rhizosphere growth-promoting technology, applied in the field of plant microorganisms, to achieve the effects of increasing the molding rate, promoting growth, increasing the number of germination and crown width

Active Publication Date: 2016-07-20
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are research reports about inoculating plant rhizosphere growth-promoting bacteria to improve the survival rate and seedling q...

Method used

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  • Herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa and application thereof
  • Herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa and application thereof
  • Herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening and Identification of Example 1 Bacterial Strain JWP7

[0023] The screening and isolation process of Pseudomonas aeruginosa (Pseudomonasaeruginosa) JWP7 is as follows:

[0024] (1) Collect 12 soil samples from the rhizosphere of the sedum succulent plant in the sedum succulent plant production base of Huanglou Town, Qingzhou City, Shandong Province, weigh 10g of the soil sample respectively, add 90ml sterile water, 37 ℃, 180r / min, and cultured on a shaking table for 30 min. After gradient dilution, 0.1ml of the bacterial solution was applied to LB medium and Gao's No. 1 medium respectively, and a single colony was obtained after culture.

[0025] (2) pick single bacterial colony three zone lines to purify bacterial classification, in organophosphorus medium (glucose 10.0g; (NH4) 2 SO 4 0.5g; MgSO 4 ·7H 2 O0.3g; MnSO 4 4H 2 O0.03g; KCl0.3g; FeSO 4 ·7H 2 O0.03g; NaCl0.3gCaCO 3 5.0g; Lecithin 0.2g; Distilled water 1000ml; pH7.0-7.5) and inorganic phosp...

Embodiment 2

[0034] Embodiment 2 JWP7 promotes sedum growth test

[0035] (1) Preparation of JWP7 bacterial agent

[0036] Activate the JWP7 stored on the slant in LB (peptone 10g; NaCl 10g; yeast extract 5g; agar 15-20g; water 1000ml; pH7.0-7.2, aliquot and sterilize at 121°C for 20min ) on the tablet. Pick a single colony into the corresponding 10ml liquid medium, and culture at 37°C and 180rpm for 24h. The seed solution was inoculated into corresponding 50ml liquid medium for fermentation according to 1% inoculum amount, and cultured at 37°C and 180rpm for 2 days to obtain JWP7 bacterial agent.

[0037] (2) Take Sedum sedum as the experimental material, the cutting substrate is peat and perlite with a mass ratio of 3:1, select Sedum sedum leaves of the same size, and remove them from the plant and place them in a ventilated and cool place for 10 days , Insert the leaves into the plug substrate at an angle of 45° to a depth of 1 cm, and cut 50 leaves in each plug. Dilute the JWP7 bac...

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Abstract

The invention provides herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa JWP7. The strain is collected in the China General Microbiological Culture Collection Center (CGMCC) on January 7, 2016, with collection number of CGMCC No.11969. The strain has good phosphate solubilizing capacity, can be successfully colonized in rhizosphere soil of crassulaceae succulent plants, can obviously promote growth of root systems and seedlings, subjected to leaf cutting, of the crassulaceae succulent plants, increase the germination number and crown diameter of herb of common stonecrop and increase the shaping rates of the crassulaceae plants and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant microorganisms, and in particular relates to a sedum rhizosphere growth-promoting Pseudomonas aeruginosa and its application. Background technique [0002] Crassulaceae is a family of dicotyledonous plants. The plants of this family are perennial succulent herbs that bloom in summer and autumn. The flowers are small and luxuriant. The epidermis has waxy powder and the stomata are sunken to reduce transpiration. It is a typical xerophyte with strong asexual reproduction. The sedum plants are short, and because they are fleshy, they consume little water and fertilizer, so they are very easy to grow and watch. In recent years, the market demand for sedum succulents has grown rapidly. The annual market demand for succulents used only for interior decoration has exceeded 100 million pots, and the annual output value has exceeded 500 million yuan. [0003] Leaf cutting propagation is currently the main ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/385
CPCA01N63/10C12N1/20C12N1/205C12R2001/385
Inventor 刘方春马海林丁延芹杜秉海马丙尧杜振宇姚良同李丽单连娟
Owner SHANDONG FOREST SCI RES INST
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