Dendrobium officinale rapid propagation tissue culture medium and preparation method thereof

Abstract

The invention relates to the technical field of culture media, in particular to a dendrobium officinale rapid propagation tissue culture medium and a preparation method thereof. 60-100 g/L of bark powder is added into a cluster bud induction culture medium, 5-20 g/L of bark powder is added into a rooting culture medium, and the relative content of the bark powder in a 1/2 MS culture medium is changed, so that the promotion effect on a cluster bud induction stage and a rooting stage of dendrobium officinale tissue culture seedlings is guaranteed, promotion stages of the cluster bud induction stage and the rooting stage of the dendrobium officinale tissue culture seedlings are optimized respectively, the seedling emergence number of the dendrobium officinale seedlings is increased, and the seedling emergence rate and the transplanting survival rate are increased; and meanwhile, the yellowing adaptation process of transferring the dendrobium officinale seedlings into different culture media is also shortened.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a rapid propagation tissue culture medium of Dendrobium officinale and a preparation method thereof. Background technique [0002] Dendrobium candidum, also known as black knot grass, is an annual herb and a rare medicinal plant. "Compendium of Materia Medica" records: Dendrobium tonifies viscera, fatigue and thinness, strengthens yin and improves essence, long-term use, thickens the stomach, nourishes internal deficiency, improves eyebrows, grows muscles, improves intelligence and eliminates shock, lightens body and prolongs life. "Dao Zang" records: Dendrobium candidum ranks first among the "nine immortal grasses" in China. The medicinal part of Dendrobium officinale is the fresh or dried stem. Modern medicine shows that Dendrobium officinale can significantly improve the immune function of the body, anti-aging, anti-fatigue, resistance to hypoxia, and has the effects ...

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Problems solved by technology

[0006] It can be seen that although the prior art has solved the high cost of artificial seedling cultivation of Dendrobium officinale to a certain extent, the long cycle, and the defects such as low transplanting survival rate, the raw material components added in the culture medium are complicated, and the cost of the raw material components used is relatively high. As a result, the cultivation cost of Dendrobium officinale seedlings is still low

[0007] In view of this, this researcher combines the practical experience in the tissue culture research of Dendrobium candidum for many years to carry out the research on the raw material components of the Dendrobium candidum medium. The traditional supplement of potato juice, banana juice, sodium molybdate, boric acid, nicotinic acid and other ingredients solves the high cost of traditional medium, and the quality of Dendrobium officinale seedlings cultured on the obtained medium is not ideal, and the survival rate is still maintained at 93% when transplanted. % and other defects provide new ideas for the culture medium of Dendrobium officinale

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