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Culture medium composition for tissue culture of crassulaceae succulent plants and application of culture medium composition

A succulent plant and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of long cycle and difficult asexual reproduction of sedum plants, and achieve the effect of promoting tissue rooting and increasing the proliferation rate

Inactive Publication Date: 2021-08-10
龙岩市禾康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the breeding method of Crassulaceae is mainly asexual reproduction, mainly including the method of branching, stem or leaf cutting. However, the cycle required for breeding is long, and it is difficult to quickly carry out asexual reproduction of Crassulaceae plants

Method used

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  • Culture medium composition for tissue culture of crassulaceae succulent plants and application of culture medium composition
  • Culture medium composition for tissue culture of crassulaceae succulent plants and application of culture medium composition

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Embodiment 1

[0022] The culture medium composition of present embodiment 1 comprises induction medium, proliferation medium, strong seedling medium and rooting medium, wherein, induction medium comprises MS medium, agar, the glucose of 25g / L, the glucose of 0.3mg / L NAA, 2mg / L of 6-BA and 3mg / L of nisin, control the pH of the induction medium between 5.4-5.5; the proliferation medium includes B5 medium, agar, 35g / L of glucose, 0.4mg / L L of NAA, 1mg / L of 6-BA and 40mg / L of sodium hypochlorite, control the pH of the proliferation medium between 5.5-5.6; strong seedling medium includes N6 medium, agar, 0.7mg / L of NAA, 0.8mg / L of 6-BA and 70mg / L of calcium propionate, the pH of the strong seedling medium is controlled between 5.5-5.6; the rooting medium includes MS medium, agar, 0.8mg / L of NAA and 0.9mg / L 6-BA, control the pH of the rooting medium between 5.6-5.7.

[0023] In this embodiment 1, the MS medium in the induction medium and the rooting medium is the MS medium disclosed by Murashig...

Embodiment 2

[0025] The culture medium composition of present embodiment 2 comprises induction medium, proliferation medium, strong seedling medium and rooting medium, wherein, induction medium comprises MS medium, agar, the glucose of 40g / L, the glucose of 0.8mg / L NAA, 3mg / L 6-BA and 5mg / L nisin, control the pH of the induction medium between 5.7-5.8; the proliferation medium includes B5 medium, agar, 50g / L glucose, 0.6mg / L L of NAA, 2mg / L of 6-BA and 60mg / L of sodium hypochlorite, control the pH of the proliferation medium between 5.9-6; strong seedling medium includes N6 medium, agar, 0.9mg / L of NAA, 1.2mg / L of 6-BA and 90mg / L of calcium propionate, control the pH of the strong seedling medium between 6.1-6.2; the rooting medium includes MS medium, agar, 0.9mg / L of NAA and 1.3mg / L 6-BA, control the pH of the rooting medium between 5.9-6.

[0026] In this embodiment 2, the MS medium in the induction medium and the rooting medium is the MS medium disclosed by Murashige and Skoog in 1962...

Embodiment 3

[0028] The culture medium composition of present embodiment 3 comprises induction medium, proliferation medium, strong seedling medium and rooting medium, wherein, induction medium comprises MS medium, agar, the glucose of 30g / L, the glucose of 0.5mg / L NAA, 2.6mg / L of 6-BA and 4mg / L of nisin, control the pH of the induction medium between 5.6-5.7; the proliferation medium includes B5 medium, agar, 40g / L of glucose, 0.5mg / L of NAA, 1.2mg / L of 6-BA and 50mg / L of sodium hypochlorite, control the pH of the proliferation medium between 5.7-5.8; strong seedling medium includes N6 medium, agar, 0.8mg / L of NAA, The 6-BA of 1mg / L and the calcium propionate of 80mg / L, control the pH of strong seedling medium between 5.7-5.8; Rooting medium includes MS medium, agar, NAA of 0.83mg / L and 1.1mg / L L of 6-BA, control the pH of the rooting medium between 5.7-5.8.

[0029] In this embodiment 3, the MS medium in the induction medium and the rooting medium is the MS medium disclosed by Murashig...

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Abstract

The invention relates to the technical field of plant tissue culture, and particularly discloses a culture medium composition for tissue culture of crassulaceae succulent plants and application of the culture medium composition. The culture medium composition comprises an induction culture medium, a proliferation culture medium, a seedling strengthening culture medium and a rooting culture medium, wherein the induction culture medium comprises the following raw materials: an MS culture medium, 25-40 g / L of glucose, 0.3-0.8 mg / L of NAA, 2-3 mg / L of 6-BA and 3-5 mg / L of nisin; the proliferation culture medium is prepared from the following raw materials: a B5 culture medium, 35-50g / L of glucose, 0.4-0.6 mg / L of NAA, 1-2mg / L of 6-BA and 40-60 mg / L of sodium hypochlorite; the seedling strengthening culture medium is prepared from the following raw materials: an N6 culture medium, 0.7-0.9 mg / L of NAA, 0.8-1.2 mg / L of 6-BA and 70-90 mg / L of calcium propionate; and the rooting culture medium is prepared from the following raw materials: an MS culture medium, 0.8-0.9 mg / L of NAA and 0.9-1.3 mg / L of 6-BA. The culture medium composition can be used for tissue culture of the crassulaceae succulent plants and has the advantage of quickly performing asexual propagation on the crassulaceae plants.

Description

technical field [0001] The present application relates to the technical field of plant tissue culture, more specifically, it relates to a medium composition for tissue culture of Crassulaceae succulent plants and its application. Background technique [0002] Crassulaceae is a family of dicotyledonous plants. This family of plants is a perennial succulent herb that blooms in summer and autumn. The flowers are small and luxuriant. The epidermis has waxy powder and the stomata are sunken, which can reduce transpiration. Strong fecundity, picking leaves can be planted and rooted. [0003] The economic value of sedum plants is mainly reflected in two aspects: one is the ornamental value, sedum plants have the characteristics of drought tolerance, cold resistance, barren resistance, less pests and diseases, low maintenance, extensive management, etc., which can save water, energy, and It is one of the indispensable plant species to create economical gardens and urban greening. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 樊昌华张玉华樊钰书樊小燕
Owner 龙岩市禾康生物科技有限公司
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