The invention discloses a preparation process for a high-strength high-conductivity micro copper wire. The preparation process comprises the following steps: S1, adding electrolytic copper into a smelting furnace, preserving heat and melting the electrolytic copper completely to obtain molten copper, introducing an inert gas, adding a composite refining agent, standing and slagging; S2, detecting that the molten copper comprises the following components in percentage by weight: 0.45 to 0.55 percent of Ti, 0.25 to 0.35 percent of Zr, 0.07 to 0.09 percent of Te, 0.07 to 0.09 percent of La, 0.03 to 0.05 percent of Y, 0.15 to 0.25 percent of Sn, less than or equal to 0.05 percent of Mg, and the balance of Cu; S3, enabling a crystallizer of a continuous casting machine to extend into the molten copper, and drawing upwards through a drawing roller mechanism to form an oxygen-free copper rod; S4, extruding the oxygen-free copper rod continuously and drawing repeatedly to obtain the high-strength high-conductivity micro copper wire. According to the copper wire which is prepared by the preparation process, the electric conductivity reaches over 95 percent IACS, and moreover, the tensile strength is also over 480 MPa.