Preparation method of immobilization bacterium for restoring polycyclic aromatic hydrocarbons contaminated soil
A technology of polluted soil and polycyclic aromatic hydrocarbons, applied in the restoration of polluted soil, immobilized on or in the inorganic carrier, etc., can solve problems such as failure to achieve, improve immobilization efficiency, improve diffusion and mass transfer performance, The effect of a good living environment
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Example Embodiment
[0023] Example 1
[0024] (1) Liquid culture of immobilized bacteria
[0025] In the seed medium (glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 7H 2 Bacillus was inoculated into an Erlenmeyer flask with O 0.02%, KCl 0.02%, pH 6.5), and placed in a constant temperature shaker at 28-30°C for 12h.
[0026] (2) Pretreatment of immobilized carrier
[0027] The red brick powder that has passed through the 1.00mm sieve is soaked in tap water for 12 to 24 hours at a ratio of 0.5 to 1.5 ml / g, and sterilized by high-temperature steam at 121°C for 90 minutes for use.
[0028] (3) Preparation of immobilized particles
[0029] Take the bacterial suspension and evenly add it to the surface of the sterile carrier. The inoculum amount is 2%. After sealing, place it in a 28°C constant temperature incubator and cultivate in the dark. Add the growth medium (glucose 4.0%) at the amount of 1ml / g carrier every day. , Yeast extract 0.3%, KH 2 PO 4 0.05%, MgSO 4 ·7H 2 O 0.025%, (NH 4 ) 2 HPO ...
Example Embodiment
[0033] Example 2
[0034] The difference from Example 1 is that during carrier pretreatment, the fly ash after passing through a 0.25 mm sieve is soaked with tap water at a ratio of 1.5 ml / g for 12 hours, sterilized by high-temperature steam at 121°C for 90 minutes, and ready for use.
[0035]The prepared immobilized particles were put into the soil contaminated by polycyclic aromatic hydrocarbons according to the soil weight ratio of 6%. After 42 days, the removal rate of PYR in the soil was 42%; the removal rate of BaP in the soil was 36%. In the entire degradation process, the degradation rate of the immobilized particles is fast, and the immobilized particles still have more than 90% activity after 42 days.
Example Embodiment
[0036] Example 3
[0037] The difference from Example 1 is that the vermiculite that has passed through a 1mm sieve is soaked with tap water at a ratio of 0.5 ml / g for 24 hours, sterilized by high-temperature steam at 121°C for 90 minutes, and then used.
[0038] The prepared immobilized particles were put into the soil contaminated by polycyclic aromatic hydrocarbons according to the soil weight ratio of 6%. After 42 days, the removal rate of PYR in the soil was 51%; the removal rate of BaP in the soil was 39%. In the entire degradation process, the degradation rate of the immobilized particles is fast, and the immobilized particles still have more than 90% activity after 42 days.
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