Antipathogenic target gene fragment of Verticillium dahliae vdeg and its interference vector and application

A technology for Verticillium dahliae and anti-pathogenic bacteria, which is applied in the fields of application, genetic engineering, DNA/RNA fragments, etc., and can solve problems such as less research on the effect

Active Publication Date: 2020-09-04
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies on exoglucanases mainly focused on enzymology, but less research on their role in pathogenicity

Method used

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  • Antipathogenic target gene fragment of Verticillium dahliae vdeg and its interference vector and application
  • Antipathogenic target gene fragment of Verticillium dahliae vdeg and its interference vector and application
  • Antipathogenic target gene fragment of Verticillium dahliae vdeg and its interference vector and application

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Experimental program
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Effect test

Embodiment 1

[0040] Example 1 Screening of Verticillium dahliae VdEG Anti-pathogenic Bacteria Target Gene Fragment, Construction of Interference Vector and Application of Transformed Tobacco Disease Resistance

[0041] ⒈Materials and methods

[0042] ⑴Materials

[0043] Tobacco: benthamiana ( Nicotiana benthamiana )strain.

[0044] Culture conditions: planted in mixed nutrient soil sterilized by high temperature and high pressure (Fangjie nutrient soil: vermiculite = 1:1), temperature 23 ± 2°C, relative humidity 75 ± 5%, photoperiod L:D is 16 h: 8 h.

[0045] ⑵ strains and plasmids

[0046] Cotton Verticillium dahliae pathogen: Verticillium dahliae ( Verticillium dahliae ) V991, a highly pathogenic deciduous strain, was donated by Jian Guiliang, a researcher at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0047] Viral vector: tobacco rattle virus ( Tobacco rattle virus , TRV) binary vector (TRV1 and TRV2) was kindly donated by Professor Liu Yule f...

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Abstract

The invention discloses an anti-pathogenic bacteria target gene fragment of verticillium dahlia VdEG and an interference vector and application thereof. A host induced gene silencing technology is adopted, a verticillium dahlia strain with high pathogenicity is used as a material to construct a plurality of interference plasmids of nicotiana fracture virus against exoglucanase of the target gene of verticillium dahlia, nicotiana benthamiana is transformed, and verticillium dahlia is inoculated by a method of agrobacterium injection, a Gateway interference vector is constructed in the target region which can obviously reduce the plant disease index to obtain stable genetic transgenic plants, and the fungal biomass and target gene transcription level are detected by the disease index and molecular biological methods to screen the best interference region. The screened VdEG target gene fragment and the Gateway interference vector constructed by adopting the target gene fragment can be applied to the improvement of the disease resistance of plants to verticillium dahlia and the cultivation of new varieties of anti-verticillium dahlia transgenic plants.

Description

technical field [0001] The present invention relates to the VdEG anti-pathogen target gene fragment of Verticillium dahliae and an interference vector containing the target gene fragment. The application in resistance to pathogenic bacteria belongs to the field of target gene fragments and applications of Verticillium dahliae against pathogenic bacteria. Background technique [0002] Verticillium dahliae ( Verticillium dahliae ) is an extremely harmful soil-borne vascular fungal disease with a wide range of hosts, which can infect cotton, sunflower, eggplant, pepper, tomato, tobacco, potato, melon, watermelon, cucumber, peanut, kidney bean, mung bean, soybean, More than 200 economic crops such as sesame and sugar beet have caused great economic losses to China and the world (HillocksI R. Cotton diseases. Center Agriculture Bioscience International, 1992: 240 ~ 257.), and the microsclerotia produced in the later stage can be found in Survive for several years or even longer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24C12N15/113C12N15/82A01H5/00A01H6/82A01H6/60A01H6/34A01H6/54
CPCC12N9/2405C12N15/113C12N15/8218C12N15/8282
Inventor 苏晓峰郭惠明程红梅
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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