Method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase

A carbonic anhydrase, catalytic preparation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of ecological environment destruction, environmental pollution, high energy consumption, etc., and achieve low cost, clean environment, high purity effect

Inactive Publication Date: 2011-07-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemical processing methods mainly produce light calcium carbonate, including carbonization method, soda calcium chloride method, caustic soda method, combined calcium method and Solvay method, etc. The main disadvantages of these methods are high energy consumption and easy En

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  • Method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase
  • Method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase
  • Method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase

Examples

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Effect test

Embodiment 1

[0027] Preparation of microbial extracellular carbonic anhydrase and its catalytic preparation of calcium carbonate in a liquid system containing calcium chloride and sodium bicarbonate solution. Inoculate Bacillus cereus (Bacillus pasteurii) in bacterial culture medium supplemented with 10 μM zinc sulfate, culture at 30°C and 120 r / min for 24 hours, collect the supernatant after refrigerated centrifugation at 5000 r / min for 15 minutes, and refrigerate the bacteria Standby, the supernatant was salted out with 70% saturated ammonium sulfate for 12 hours, centrifuged to get the protein precipitate, and the protein precipitate was dissolved in 0.2mol / L phosphate buffer solution of pH8.0, passed through the G100 gel column, and washed with 0.2mol / L pH8.0 phosphate buffer for elution, collect the part with high enzyme activity, put it in a dialysis bag for dialysis for 24 hours, then pass the dialysate through a DEAE-cellulose ion exchange column, first use 0.2mol / L pH8.0 Equilibr...

Embodiment 2

[0029]Preparation of microbial intracellular carbonic anhydrase and its catalytic preparation of calcium carbonate in a liquid system containing calcium chloride and sodium bicarbonate solution. The thallus obtained in Example 1 was added to 0.2mol / L pH8.0 phosphate buffer to make the thalline concentration 10%, ground for 30 minutes under ice-bath conditions, refrigerated and centrifuged for 15 minutes at 5000rpm, and the supernatant was taken solution, the supernatant was salted out with 70% saturated ammonium sulfate for 12 hours, and the subsequent steps were the same as the preparation method of extracellular carbonic anhydrase described in Example 1 to obtain an intracellular carbonic anhydrase solution, and detect its enzyme activity It is 8.2U / mL. Prepare sodium bicarbonate and calcium chloride solution and place in the Erlenmeyer flask, the concentration of sodium bicarbonate and calcium chloride is 25.2mmol / L, add the intracellular carbonic anhydrase solution of prep...

Embodiment 3

[0031] Microbial extracellular carbonic anhydrase catalyzes the production of calcium carbonate in a gas-diffusion system. The preparation method of extracellular carbonic anhydrase was the same as that in Example 1, and its enzyme activity was detected to be 8.0 U / mL. In a closed glass desiccator, place a 50mL small beaker on the upper layer, which contains 25.0mmol / L calcium chloride solution, in which extracellular carbonic anhydrase is added, the enzyme concentration is 1.8U / mL, and the initial pH is controlled at 6.5; The gas-permeable partition is bounded; the lower layer is filled with 400mL 0.1mol / L ammonium bicarbonate solution. Put the whole desiccator at 20°C and 100r / min to shake and react for 24 hours, take out the generated precipitate, filter, wash and dry to obtain calcium carbonate.

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Abstract

The invention provides a method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase, which comprises the following steps of: adding the carbonic anhydrase extracted from a microbial culture into a liquid system containing calcium ion and bicarbonate radical ion solution or a gas diffusion system containing calcium ion solution and releasing CO2 by using ammonium hydrogen carbonate solution, performing oscillating reaction at room temperature, controlling initial pH at 5 to 10, taking out the produced sediment, and performing filtration, washing and drying to obtain the calcium carbonate. The method makes full use of microbial resources and characteristics of specificity and high efficiency of enzyme catalytic reaction, prepares the calcium carbonate by accelerated deposition, and has the advantages of riche resources, simple process, clean environment, low cost, high efficiency, quickness and the like. The calcium carbonate prepared by deposition has high purity, is calcite crystal calcium carbonate, can be used as an industrial filler, and can also be used for repairing cracks of building engineering, buildings, stone landscapes and carved stone cultural relics.

Description

technical field [0001] The invention relates to a method for preparing calcium carbonate, in particular to a method for preparing calcium carbonate catalyzed by microbial carbonic anhydrase. Background technique [0002] Calcium carbonate is an important inorganic mineral and the most abundant mineral resource in the earth. Calcium carbonate is not only widely used in rubber, plastic, and coating industries, but also in ink, paper, cable, medicine, daily chemical industries, and is also widely used in environmental protection fields such as sulfur removal and sewage treatment. In addition, studies have shown that calcium carbonate can play an important role in the protection and restoration of stone landscapes and stone carvings, as well as in the repair of cracks in major construction projects and important historical buildings. [0003] At present, there are mainly two methods for the preparation of calcium carbonate, namely chemical processing and mechanical methods. Ch...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/085
Inventor 李为余龙江曹龙陈薇珊刘丽萍敖明章栗茂腾
Owner HUAZHONG UNIV OF SCI & TECH
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