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Nano carrier for tumor photo-dynamics therapy (PDT) and preparation method thereof

A photodynamic therapy, nanocarrier technology, applied in photodynamic therapy, nanotechnology for sensing, preparations for in vivo experiments, etc., can solve the problem of reduced oxygen concentration, low photodynamic therapy efficiency, and low ROS range and other problems to achieve the effect of increasing oxygen content, enhancing photodynamic therapy, good biocompatibility and biodegradability

Inactive Publication Date: 2018-12-07
HUBEI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although photodynamic therapy has many advantages in the treatment of tumors, it still faces many problems in its wide application: the effect of existing photodynamic therapy is affected by some factors, mainly the hypoxic environment of the tumor itself, low concentration The photodynamic therapy of tumors is an oxygen-consuming process, which leads to a further decrease in the oxygen concentration in tumor cells. Without photosensitizers, it is difficult to produce sufficient reactive oxygen species to induce cell necrosis or apoptosis. , leading to low efficiency of photodynamic therapy

Method used

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  • Nano carrier for tumor photo-dynamics therapy (PDT) and preparation method thereof
  • Nano carrier for tumor photo-dynamics therapy (PDT) and preparation method thereof
  • Nano carrier for tumor photo-dynamics therapy (PDT) and preparation method thereof

Examples

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Embodiment 1

[0025] 1. In a 250mL flask, 2mLFe 3+ The solution (0.0125mM) was added to 10mL of DMF, then 2mL of TCPP solution (0.0125mM) was added dropwise, and 0.4mL of acetic acid was added to adjust the pH of the mixed solution to an acidic environment. After stirring vigorously at room temperature for 2 hours, the mixed solution was heated to 80°C and stirred slowly for 24 hours. After the reaction, the solution was naturally cooled to room temperature, centrifuged, washed with DMF, ethanol, and deionized water to remove residual solvents, and the purified NMOFs were stored at 4 °C. All reactions were performed in the dark.

[0026] 2. Take 2 mL of BSA solution (0.1 mM) and add it to 800 microliters of SDs solution (1 mM), and stir at room temperature for 12 hours to obtain a BSA / SDs mixture. 100 mg of NMOFs prepared in S1 were ultrasonically dispersed in deionized water for 2 hours, then 40 mg of EDC and 30 mg of NHS were added immediately under magnetic stirring at 40 °C, and the B...

Embodiment 2

[0029] Step 1. In a 250mL flask, 2mLFe 3+The solution (0.0127mM) was added to 15mL of DMF, then 2mL of TCPP solution (0.0127mM) was added dropwise, and 0.5mL of acetic acid was added to adjust the pH of the mixed solution to an acidic environment. After stirring vigorously at room temperature for 2 hours, the mixed solution was heated to 80°C and stirred slowly for 24 hours. After the reaction, the solution was naturally cooled to room temperature, centrifuged, washed with DMF, ethanol, and deionized water to remove residual solvents, and the purified NMOFs were stored at 4 °C. All reactions were performed in the dark.

[0030] Step 2. Take 2 mL of BSA solution (0.1 mM) and add it to 800 microliters of SDs solution (1 mM), and stir at room temperature for 12 hours to obtain a BSA / SDs mixture. 100 mg of NMOFs prepared in S1 were ultrasonically dispersed in deionized water for 2 hours, then 40 mg of EDC and 30 mg of NHS were added immediately under magnetic stirring at 40 °C, ...

Embodiment 3

[0033] 1. In a 250mL flask, 2mLFe 3+ The solution (0.013mM) was added to 15mL of DMF, then 2mL of TCPP solution (0.013mM) was added dropwise, and 0.5mL of acetic acid was added to adjust the pH of the mixed solution to an acidic environment. After stirring vigorously at room temperature for 2 hours, the mixed solution was heated to 80°C and stirred slowly for 24 hours. After the reaction, the solution was naturally cooled to room temperature, centrifuged, washed with DMF, ethanol, and deionized water to remove residual solvents, and the purified NMOFs were stored at 4 °C. All reactions were performed in the dark.

[0034] 2. Take 2 mL of BSA solution (0.1 mM) and add it to 800 microliters of SDs solution (1 mM), and stir at room temperature for 12 hours to obtain a BSA / SDs mixture. 100 mg of NMOFs prepared in S1 were ultrasonically dispersed in deionized water for 2 hours, then 40 mg of EDC and 30 mg of NHS were added immediately under magnetic stirring at 40 °C, and the BSA...

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Abstract

The invention provides a preparation method of a nano carrier for tumor photo-dynamics therapy (PDT). According to the preparation method, Fe<3+> salts are dissolved in DMF, then a photo-sensitizer (TCPP) is added to obtain particles (NMOFs); then the particles are dispersed in water under the assistance of ultrasonic waves; crosslinking agents (EDC and NHS) are added; after the reactions betweenBSA and sulfadiazine (SDs) finish completely, dialysis is performed to obtain particles (NMOFs@BSA / SDs); then the particles are dispersed in distilled water, then Mn<2+> salts are added, the pH is adjusted, and finally dialysis is performed to obtain a carrier (NMOFs@BSA / SDs@MnO2). Nano metal organic framework particles are taken as the basis and coated by protein (BSA) and sulfadiazine (SDs); finally the particles are in-situ mineralized to obtain required particles; SDs can specifically recognize carbonic anhydrase of tumors and is capable of actively targeting the oxygen-deficient parts oftumors; MnO2 generated in mineralization can catalyze the H2O2 decomposition to increase the oxygen content of tumors; and the PDT efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of bio-targeted nano-medicine, in particular to a nano-carrier for photodynamic therapy of tumors and a preparation method thereof. Background technique [0002] my country is one of the countries with high incidence of tumor diseases, and about a quarter of cancer deaths in the world occur in China. Deep tumors in the body, such as primary cellular hepatocellular carcinoma (HCC), ranks second among high-mortality tumors in my country. Most HCC patients in my country have a background of hepatitis B and liver cirrhosis. The onset is hidden, and early symptoms are mostly not obvious. Early detection The rate is low, surgical margins are difficult to define, postoperative recurrence rate is high, chemotherapy and postoperative chemotherapy are not highly targeted and toxic. Therefore, improving the targeting of deep tumor treatment, reducing skin phototoxicity with minimally invasive treatment, and preventing ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K31/63A61K49/14A61K49/18A61P35/00B82Y5/00B82Y15/00A61K47/42A61K47/02
CPCA61K9/5115A61K31/63A61K41/0057A61K47/42A61K49/143A61K49/1818A61P35/00B82Y5/00B82Y15/00A61K2300/00
Inventor 易昌凤操金国杨盛力朱伟
Owner HUBEI UNIV
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