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Method for preparing calcium carbonate by simultaneous mineralization of two microorganisms

A technology of microorganisms and calcium carbonate, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of pollution and slow mineralization rate, and achieve the effects of environmental friendliness, high preparation efficiency and simple operation

Inactive Publication Date: 2018-06-29
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] In the existing invention, the microbial mineralization deposition technology only uses a kind of microorganism to mineralize and deposit calcium carbonate. Although the mineralization rate is fast when the urease microorganism is used alone, the ammonia produced by it has a polluting effect on the environment. The carbonic anhydrase used only Microbes capture atmospheric CO 2 , environmentally friendly, but its mineralization rate is relatively slow

Method used

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  • Method for preparing calcium carbonate by simultaneous mineralization of two microorganisms
  • Method for preparing calcium carbonate by simultaneous mineralization of two microorganisms
  • Method for preparing calcium carbonate by simultaneous mineralization of two microorganisms

Examples

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Effect test

Embodiment 1

[0048] A kind of method that utilizes two kinds of microorganisms to mineralize jointly to prepare calcium carbonate, the steps are as follows:

[0049] (1) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid to make a Tris-Hcl buffer solution with a pH of 9.0, then weigh the yeast extract 20g of ammonium sulfate, 10g of ammonium sulfate, dissolved in 1LTris-Hcl buffer; preparation of culture medium for Bacillus pumilus: Weigh 5g of peptone, 1.5g of beef extract, 1.5g of yeast extract, 5g of NaCl, and dissolve in 1L of distilled water Adjust the pH to 8.0. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0050] (2) Take by weighing 166.5g of anhydrous calcium chloride, 60.1g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed soluti...

Embodiment 2

[0055] A kind of method that utilizes two kinds of microorganisms to mineralize jointly to prepare calcium carbonate, the steps are as follows:

[0056] (1) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid to make a Tris-Hcl buffer solution with a pH of 9.0, then weigh the yeast extract 20g of ammonium sulfate, 10g of ammonium sulfate, dissolved in 1LTris-Hcl buffer; preparation of culture medium for Bacillus pumilus: Weigh 5g of peptone, 1.5g of beef extract, 1.5g of yeast extract, 5g of NaCl, and dissolve in 1L of distilled water Adjust the pH to 8.0. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0057] (2) Take by weighing 166.5g of anhydrous calcium chloride, 60.1g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed soluti...

Embodiment 3

[0062] A kind of method that utilizes two kinds of microorganisms to mineralize jointly to prepare calcium carbonate, the steps are as follows:

[0063] (1) Preparation of culture medium for Bacillus sarcina pasteurii: Weigh 15.75g of Tris and dissolve it in 1L of distilled water, adjust the pH with 50% hydrochloric acid to make a Tris-Hcl buffer solution with a pH of 9.0, then weigh the yeast extract 20g of ammonium sulfate, 10g of ammonium sulfate, dissolved in 1L Tris-Hcl buffer; preparation of culture medium for Bacillus pumilus: Weigh 5g of peptone, 1.5g of beef extract, 1.5g of yeast extract, 5g of NaCl, and dissolve in 1L of distilled water Adjust the pH to 8.0. The prepared liquid medium was divided into Erlenmeyer flasks, sterilized at 121°C for 20 minutes, and then taken out for use.

[0064] (2) Take by weighing 166.5g of anhydrous calcium chloride, 60.1g of urea is dissolved in 1L distilled water, mix well, the concentration of calcium chloride in this mixed solut...

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Abstract

The invention relates to a method for preparing calcium carbonate by simultaneous mineralization of two microorganisms. The method is characterized by comprising the following steps: adding a culturesolution of the two cultured microorganisms to a mixed solution of calcium salt and urea, putting a reactor in an open mode to enable the solution to be exposed to the air to be subjected to mineralization reaction, taking out the precipitate after the reaction is completed, and washing and drying the precipitate to obtain a calcium carbonate product. The two microorganisms are respectively sporosarcina pasteurii belonging to a urease high-yield strain and bacillus pumilus belonging to a carbonic anhydrase high-yield strain. The method provided by the invention has the advantages that the twostrains together participate in the mineralization process, the generation efficiency of calcium carbonate is increased, simultaneously, the pollution to the environment caused by generation of ammonia gas can be reduced, CO2 in the atmosphere can also be captured, and the advantages of the two strains are complementary so as to cooperatively promote the whole mineralization process; the microorganisms utilized in the method are rich in resources, efficient and single in enzyme catalysis reaction, simple in production process and low in cost; and furthermore, the obtained calcium carbonate product is higher in purity, has better cementing action and can be widely applied to the field of civil engineering.

Description

technical field [0001] The invention relates to a method for preparing calcium carbonate, in particular to a method for preparing calcium carbonate by using microbial mineralization reaction. In the present invention, the joint mineralization of two microorganisms is used to prepare calcium carbonate. Background technique [0002] Calcium carbonate is an important inorganic chemical material and a typical biomineral. It is widely used in different fields such as rubber, plastic, paper, ink, paint, medicine, environmental protection, and food. The commonly used calcium carbonate preparation methods can be divided into mechanical pulverization method and chemical reaction method. The mechanical pulverization method is usually to pulverize natural limestone and white stone to obtain calcium carbonate. The calcium carbonate obtained by this method is heavy calcium carbonate with a large particle size, generally in the micron range. Light calcium carbonate is produced by chemica...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12R1/01
CPCC12P3/00C12P39/00
Inventor 韩培培李梦楠张晓琪贾士儒谭之磊钟成
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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