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Method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves

A technology for the simultaneous separation and separation of leucin, applied in chemical instruments and methods, separation/purification of carboxylic acid esters, preparation of carboxylic acid esters, etc., can solve the problem that flavonoid monomers and chlorogenic acid cannot be separated simultaneously from leaves of white hemp leaves and other issues, to achieve the effects of improving production efficiency and raw material utilization, stable properties, high production and practical value

Inactive Publication Date: 2018-12-18
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology allows us to separate these two compounds effectively while maintaining their purity at very high levels. It also makes it easier to produce them economically due to its simplicity compared to previous methods that required multiple steps or expensive materials.

Problems solved by technology

The technical problem addressed in this patented paper relates to separating and purifying different types of natural or synthetic chemical substances called lutene classified into specific groups based upon their structure characteristics such as hydroxy groupings at certain positions within them.

Method used

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  • Method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves
  • Method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves
  • Method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, after crushing 500 g of dried white basil leaves, add 1000 mL of 70% ethanol solution to heat and reflux for extraction twice, and the combined extracts are distilled under reduced pressure to obtain 51.34 g of big-leaf white basil leaves; Add the extract to 500mL distilled water for ultrasonic dissolution, filter out the insoluble matter, and the filtrate flows through the HPD-300 macroporous resin column at a flow rate of 3BV / h; after the adsorption is completed, the HPD-300 macroporous resin column is fully eluted with 5BV distilled water first, Collect the eluate from the first stage, concentrate and freeze-dry to obtain 5.36g of chlorogenic acid with a purity of 53.3%; HPD-300 macroporous resin column is fully eluted with 5BV 20% ethanol aqueous solution, and the second stage eluted liquid, obtained 4.63g product by distillation under reduced pressure; this product was dissolved with 30mL methanol, loaded on the SephadexLH-20 gel chromatographic column, e...

Embodiment 2

[0020] Example 2, after crushing 2500g of dried white basil leaves, add 5000mL of 70% ethanol solution to heat and reflux for extraction twice, and combine the extracts to obtain 249.33g of big-leaf white basil leaf extracts through vacuum distillation; Add 2500mL distilled water to the extract for ultrasonic dissolution, filter out insoluble matter, and the filtrate flows through the HPD-400 macroporous resin column at a flow rate of 2BV / h; after the adsorption is completed, the HPD-400 macroporous resin column is fully eluted with 7BV distilled water first, After collecting the first-stage eluate and concentrating and freeze-drying, 26.76g of chlorogenic acid with a purity of 52.5% was obtained; the HPD-400 macroporous resin column was fully eluted with 7BV 40% ethanol aqueous solution, and the second-stage eluate was collected , obtain 22.78g product through vacuum distillation; Dissolve this product with 150mL methanol, load on the Sephadex LH-20 gel chromatographic column,...

Embodiment 3

[0021] Example 3, after crushing 5,000 g of dried white basil leaves, add 10,000 mL of 70% ethanol solution to heat and reflux for extraction twice, and the combined extracts are distilled under reduced pressure to obtain 490.66 g of big-leaf white basil leaf extracts; Add the extract to 5000mL distilled water for ultrasonic dissolution, filter out the insoluble matter, and the filtrate flows through the HPD-200A macroporous resin column at a flow rate of 2BV / h; The eluate from the first stage was collected, concentrated and freeze-dried, and 52.48g of chlorogenic acid with a purity of 52.0% was obtained; the HPD-200A macroporous resin column was fully eluted with 10BV 100% ethanol aqueous solution, and the second stage was collected. liquid, obtained 40.07g product through vacuum distillation; this product was dissolved with 300mL methanol, loaded on the Sephadex LH-20 gel chromatography column, eluted with methanol, and the monomer eluate was collected respectively, and obtai...

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Abstract

The invention discloses a method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves. The method comprises a macro-porous resincolumn adsorption process, an eluent stepwise elution process and a gel chromatographic column refining process. The Poacynum hendersonii leaves are used as a raw material, and are processed to obtainPoacynum hendersonii leaf extract, the extract is fully dissolved in distilled water, insoluble substances are filtered out, the obtained filtrate goes through a macro-porous resin column and undergoes stepwise elution with an eluent, and the obtained first-stage eluate is concentrated and freeze-dried to obtain chlorogenic acid having a purity of 52% or above; and the obtained second-stage eluate undergoes reduced pressure distillation, and the obtained product is dissolved in methanol, and is continuously purified by a gel chromatographic column to obtain the quercetin-3-O-sophoroside having a purity of 93% or above and isoquercetin having a purity of 97% or above. The above separation method allows highly pure quercetin-3-O-sophoroside (93% or above), isoquercetin (97% or above ) and chlorogenic acid (52% or above) to be simultaneously obtained in a simple process, so the method has the advantages of simple and feasible process, high production efficiency, high raw material utilization rate, suitableness for industrial production, and high production and practical values.

Description

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Claims

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Application Information

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Owner LUDONG UNIVERSITY
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