Preparation method and application of folium apocyni veneti extract

A technology of extracts and extracts, which is applied in the field of medicine to achieve the effects of high development value, low cost, and easy operation

Inactive Publication Date: 2015-04-08
CHANGCHUN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pH range of the extract is 3~6; the detection wavelength of high performance liquid chro

Method used

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  • Preparation method and application of folium apocyni veneti extract
  • Preparation method and application of folium apocyni veneti extract
  • Preparation method and application of folium apocyni veneti extract

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preparation example Construction

[0027] The preparation method of the present invention is:

[0028] a. Grind the dried apocynum leaves and pass through a 20-50 mesh sieve to make a medicinal powder, extract with 5-20 times the amount of 20-70% ethanol aqueous solution, add sulfuric acid or hydrochloric acid to the extract, and make the pH value of the above-mentioned acid 2.5 to 3.8, the extraction temperature is 50~300 o C, each extraction time is 0.5~2 hours, extract 1~3 times in the same way, combine the extracts, filter, recover the ethanol and concentrate the combined extract solution to 1 / 4~1 / 10 of the original volume to obtain Concentrate, in the extraction process, the solid-liquid ratio of Apocynum apocynum leaves to ethanol aqueous solution is 1:10~1:20;

[0029] b, the concentrated solution of step a was left still for 3 to 24 hours, filtered, and the filtrate was separated by centrifugation at 10000 rpm, and the supernatant obtained after centrifugation was taken to go up the macroporous resin c...

Embodiment

[0044] Example 1 (existing method extraction)

[0045] Apocynum leaf medical material is pulverized, takes by weighing 100 g, puts in the extraction container, adds 1000ml volume ratio and is 60% ethanol aqueous solution, with 100 o C temperature extraction, extraction 3 times, 1 hour each time, combined extracts, reclaimed ethanol, concentrated to 1 / 5 of the original volume, concentrated solution stood still for 10 hours, supernatant on 100 g dry macroporous resin column, first Elute with water until the effluent from the macroporous resin column is colorless, clear and transparent, with a pH value of 7, and then eluted with 50% ethanol aqueous solution until the ethanol solution is colorless, clear and transparent, about 5 times the column volume, collect the eluate of the ethanol aqueous solution, recover the ethanol, and dry to obtain a dry extract of apocynum leaf extract. The weight of the extract is 6.5% of the weight of Apocynum apocynum for extraction, and the total ...

Embodiment 2

[0048] Apocynum leaf medical material is pulverized, weighs 200g, puts in the extraction container, adds 2000ml volume ratio and is 60% ethanol aqueous solution, adds hydrochloric acid in extracting liquid, makes hydrochloric acid pH value be 2.5, with 150 o C temperature extraction, extraction 3 times, 1.5 hours each time, combined extracts, reclaimed ethanol, concentrated to 1 / 5 of the original volume, concentrated solution stood still for 12 hours, supernatant was placed on a 200g dry macroporous resin column, and water Elution, the effluent eluted to the macroporous resin column is colorless, clear and transparent, with a pH value of 7, and then eluted with an ethanol aqueous solution with a volume ratio of 60%, until the ethanol solution is colorless, clear and transparent, about 5 times Column volume, collect the eluate of ethanol aqueous solution, reclaim ethanol, dry, obtain a kind of apocynum leaf extract of dry extract. The weight of the extract is 6.8% of the weight...

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Abstract

The invention relates to a preparation method and application of a folium apocyni veneti extract, belongs to the field of medicines and aims at increasing the extraction content of quercetin-3-O-beta-D-glucosyl-beta-D-glucoside and quercetin-3-O-beta-D-glucuronide in folium apocyni veneti by 0.5-10 percent and applying the quercetin-3-O-beta-D-glucosyl-beta-D-glucoside and the quercetin-3-O-beta-D-glucuronide to a medicament for treating depression. According to the folium apocyni veneti extract, the total flavonoids content is 58-78 percent, wherein the contained flavonoids component is prepared from the following components in percentage by weight: 5.0-25 percent of the quercetin-3-O-beta-D-glucosyl-beta-D-glucoside, 0.5-10 percent of the quercetin-3-O-beta-D-glucuronide, 0.5-20 percent of rutin, 5.0-25 percent of hyperin, 5.0-20 percent of isoquercetin, 2.0-10 percent of quercetin, 2.0-10 percent of kaempferol and the balance of other flavonoids components. The preparation method and the application are convenient and efficient, are easy in operation and low in cost, can meet development and utilization of the fields of the medicines and foods and have very large application and development values.

Description

technical field [0001] The invention belongs to the field of medicine. Background technique [0002] The traditional Chinese medicine Apocynum Apocynum is a traditional medicine for calming the liver and calming the wind. It has the functions of calming the liver and calming the nerves, nourishing blood and regulating menstruation, astringing yin and antiperspirant. pharmacological effects. In recent years, a large number of products such as "Apocynum tea" have appeared on the market, and they are often used clinically to treat high blood pressure or assist in lowering blood pressure. The effective parts are flavonoids. Due to the commonly used extraction methods such as "alcohol extraction-water precipitation" or "water extraction-alcohol precipitation" to extract Apocynum leaf, due to the obstruction of the plant cell wall, the chemical components in the cells are difficult to be extracted by water or ethanol solution. , due to the high content of rutin in common extract...

Claims

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Application Information

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IPC IPC(8): A61K36/24A61P25/24A61K127/00
CPCA61K36/24A61K2236/333A61K2236/39A61K2236/55
Inventor 刘春明李赛男张语迟唐英
Owner CHANGCHUN NORMAL UNIVERSITY
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