Silkworm nutritional food base material helpful for improving sleep as well as preparation method and application of silkworm nutritional food base material

Abstract

The invention discloses a preparation method of a silkworm nutritional food base material helpful for improving sleep, and the preparation method comprises the following steps: (1) selecting a bacterial liquid to carry out light fermentation on fresh mulberry leaves to obtain lightly fermented mulberry leaves; (2) from the first day of the fifth instar of the silkworm larvae to the period when the silkworm larvae stop feeding and spin, partially feeding the slightly fermented mulberry leaves every day; the preparation method comprises the following steps: freeze-drying and crushing fresh silkworm chrysalis to obtain silkworm chrysalis powder rich in gamma-aminobutyric acid; (3) mixing the silkworm chrysalis meal with fermentation auxiliary materials and water, inoculating lactobacillus rhamnosus and pediococcus pentosaceus, and fermenting to obtain a solid silkworm chrysalis fermentation product; (4) extracting the mulberry leaf powder step by step by using an ethanol solution and deionized water to obtain a mulberry leaf extract rich in gamma-aminobutyric acid, hyperoside and isoquercetin; and (5) uniformly mixing the silkworm chrysalis solid-state fermentation product and the mulberry leaf extract to obtain the silkworm and mulberry nutritional food base material. The invention further discloses a silkworm nutritional food base material beneficial to sleep improvement and application of the silkworm nutritional food base material in functional food.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and in particular relates to a silkworm and mulberry nutritional food base material which helps to improve sleep, and a preparation method and application thereof. Background Art

[0002] Sleep disorders are common physical disorders, manifested as various functional disorders in the sleep-wake process. The harm caused by sleep disorders is very serious. People with insomnia have an increased risk of developing certain types of cancer, diabetes, hypertension or obesity. Sleep disorders are closely related to mental problems such as depression. More than 70% of patients with depression have insomnia symptoms, and about 40% of people with sleep problems eventually suffer from depression.

[0003] Studies have shown that nutritional supplementation and dietary intervention can help prevent and improve sleep disorders. For example, increasing the intake of foods rich in amino acids such as tryptophan and serine can help the body synthesize neurotransmitters such as serotonin, γ-aminobutyric acid, and melatonin; increasing the intake of foods rich in antioxidant functions such as hyperoside and isoquercetin can regulate adverse factors that affect sleep disorders, such as oxidative stress and reactive oxygen species, which is of great significance for preventing sleep disorders and the various chronic diseases they induce.

[0004] In addition to producing raw silk for the textile industry, sericulture also produces a large number of silkworm pupae and mulberry leaves with extremely high food and medicinal value. Silkworms feed only on mulberry leaves throughout their lives, and the nutritional active ingredients in silkworm pupae are all transformed from the ingredients in mulberry leaves. The crude protein content in dry silkworm pupae is about 60%, of which the content of tryptophan, which has a sleep-promoting effect, is as high as 1.5g / 100g, and the content of serine is as high as 3.69g / 100g. These two amino acids can promote brain nerve cells to secrete inhibitory mediators (INH) and 5-hydroxytryptamine and other neurotransmitters, which have the effect of temporarily inhibiting the brain's thinking activities and promoting the feeling of drowsiness. In addition, studies have shown that mulberry leaves are not only rich in the sleep-promoting substance γ-aminobutyric acid, but also contain strong antioxidants such as hyperoside and isoquercetin, which are flavonoid components. Therefore, increasing the intake of mulberry leaves in the diet can not only supplement sleep-promoting substances, but also effectively improve the body's antioxidant capacity, which helps to improve sleep.

[0005] Silkworm pupae and mulberry leaves are rich in nutrition, diverse in physiological functions, safe and effective, and rich in natural functional factors that improve sleep. They are excellent natural food resources for developing functional foods that improve sleep disorders. Through modern food processing technology, the nutritional active ingredients in silkworm pupae and mulberry leaves that are beneficial to improving sleep are enriched, while their edible flavor quality is improved. Further use in the development of nutritious foods that improve sleep has a good market prospect. Summary of the invention

[0006] One of the purposes of the present invention is to provide a method for preparing a sericulture nutritional food base material which helps improve sleep.

[0007] The second object of the present invention is to provide a sericulture nutritional food base prepared by the above preparation method.

[0008] The third object of the present invention is to provide the application of the above-mentioned sericulture nutritional food base material.

[0009] The first object of the present invention is achieved through the following technical solutions.

[0010] A method for preparing a sericulture nutritious food base material that helps improve sleep, comprising the following steps:

[0011] (1) preparing mulberry leaves for silkworm breeding: selecting one of Lactobacillus plantarum, Bacillus subtilis and Leuconostoc mesenteroides and spraying it evenly on fresh mulberry leaves, then placing the mulberry leaves in a fermentation tank for short-term, anaerobic and mild fermentation, taking them out and drying the surface moisture to obtain mildly fermented mulberry leaves;

[0012] (2) Preparation of silkworm pupae: From the first day of the fifth instar of the silkworm larvae until they stop eating and spinning silk, the silkworm larvae are fed with the lightly fermented mulberry leaves 1 to 2 times a day, and are fed with ordinary fresh mulberry leaves normally during the rest of the time, or the lightly fermented mulberry leaves are used to replace part of the ordinary fresh mulberry leaves for feeding every day;

[0013] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0014] (3) Preparation of silkworm pupa solid fermentation product: mixing silkworm pupa powder with fermentation auxiliary materials and an appropriate amount of water to obtain a fermentation mixture; then inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus to ferment; and freeze-drying after the fermentation to obtain a silkworm pupa solid fermentation product;

[0015] (4) Preparation of mulberry leaf extract: crushing and pulping fresh mulberry leaves, and then freeze-drying to obtain mulberry leaf powder; adding ethanol to the mulberry leaf powder for extraction, and freeze-drying the supernatant to obtain a mulberry leaf extract A rich in hyperoside and isoquercetin;

[0016] The remaining mulberry leaf powder residue is then extracted with deionized water, and the supernatant is freeze-dried to obtain a mulberry leaf extract B rich in gamma-aminobutyric acid;

[0017] Mixing mulberry leaf extract A and mulberry leaf extract B to obtain mulberry leaf extract C rich in gamma-aminobutyric acid, hyperoside and isoquercetin;

[0018] (5) Preparation of sericulture nutritional food base: The silkworm pupae solid fermentation product and mulberry leaf extract C are mixed evenly to obtain the sericulture nutritional food base.

[0019] The sericulture nutritional food base material obtained by combining the silkworm pupa solid-state fermentation product and mulberry leaf extract C in the present invention has a good effect of improving sleep disorders and has good edible quality after the following improvements:

[0020] ①Preparing silkworm pupae raw materials rich in γ-aminobutyric acid and active peptides. The present invention uses lightly fermented mulberry leaves obtained by fermentation of Lactobacillus plantarum, Bacillus subtilis or Leuconostoc mesenteroides to feed five-year-old silkworms, and can obtain silkworm pupae with better nutritional function quality. Specifically, on the one hand, the present invention uses the above-mentioned strains to ferment mulberry leaves, which is conducive to the production of γ-aminobutyric acid therein, so that the content of γ-aminobutyric acid in the lightly fermented mulberry leaves is higher than that in ordinary mulberry leaves, thereby increasing the content of γ-aminobutyric acid in silkworm pupae; on the other hand, lightly fermented mulberry leaves carry a large number of strains. As the mulberry leaves enter the digestive tract of silkworms, based on the immune response mechanism of insects to exogenous microorganisms, the silkworm pupae obtained by feeding lightly fermented mulberry leaves to the fifth-year stage of silkworm larvae of the present invention have a significantly better content level of various small molecule active peptides such as γ-aminobutyric acid, lysozyme and antibacterial peptides in the body than the silkworm pupae obtained by feeding ordinary mulberry leaves. Animal experiments have found that the silkworm pupae obtained by feeding lightly fermented mulberry leaves of the present invention have a better sleep regulation effect on mice than the silkworm pupae obtained by feeding ordinary mulberry leaves.

[0021] ② The nutritional and flavor quality of silkworm pupae is further improved after microbial fermentation. As an edible insect, silkworm pupae have a special appearance, a small amount of fishy smell and potential allergenic risk, which are unfavorable factors affecting their eating experience. In step (3), the present invention uses Lactobacillus rhamnosus fermentation to help promote the production of silkworm pupae oligosaccharides and short-chain fatty acids such as acetic acid, propionic acid and butyric acid in the fermentation product, and at the same time promotes the release of silkworm pupae umami amino acids to enhance the umami taste of the fermentation product, thereby improving the edible flavor quality of the fermentation product; fermentation with Pediococcus pentosaceus can reduce the fishy smell of silkworm pupae, enhance the fat taste and meaty aroma, and significantly improve the edible flavor quality of the fermentation product. After fermentation, the small molecule active peptides in the solid-state fermentation product of silkworm pupae increase significantly, not only the flavor quality is significantly improved, but also the effect of improving sleep disorders is improved; experiments show that the solid-state fermentation product of silkworm pupae of the present invention has a good regulating effect on the sleep of sleep-disordered mice, and its effect is better than the silkworm pupae powder obtained in step (2).

[0022] ③ According to the polarity difference of the three components of γ-aminobutyric acid, hyperoside and isoquercetin, γ-aminobutyric acid, hyperoside and isoquercetin in mulberry leaves are respectively enriched by a two-step extraction method. The present invention uses ethanol and deionized water for step-by-step extraction, so that more γ-aminobutyric acid, hyperoside and isoquercetin in mulberry leaves are extracted, and mulberry leaf extract C rich in γ-aminobutyric acid, hyperoside and isoquercetin is obtained, which helps to improve the effect of improving sleep disorders.

[0023] ④ The combination of the silkworm pupa solid fermentation product and mulberry leaf extract C can synergistically enhance the effect of improving sleep disorders. The silkworm pupa solid fermentation product is mainly rich in functional amino acids such as γ-aminobutyric acid, tryptophan, and serine, which are helpful for the synthesis of various neurotransmitters and promote sleep, while the hyperoside and isoquercitrin in mulberry leaves improve sleep through antioxidant pathways; the combination of the two improves sleep disorders through different mechanisms of action.

[0024] In step (2), the present invention refers to feeding the silkworms with lightly fermented mulberry leaves 1 to 2 times a day instead of ordinary fresh mulberry leaves. For example, if the silkworms are fed three times a day, lightly fermented mulberry leaves are fed once and ordinary fresh mulberry leaves are fed twice, and so on. Therefore, the feeding amount of lightly fermented mulberry leaves is the same as the feeding amount of ordinary fresh mulberry leaves. Specifically, from the first day of the fifth instar of the silkworm larvae to the period when they stop eating and spinning, the cumulative feeding amount of lightly fermented mulberry leaves to each silkworm larvae accounts for about 30 to 67% of their cumulative mulberry consumption.

[0025] Preferably, when lightly fermented mulberry leaves are used to replace part of ordinary fresh mulberry leaves for feeding every day, the cumulative feeding amount of lightly fermented mulberry leaves to each silkworm larvae accounts for 30-67% of its cumulative mulberry intake from the first day of the fifth instar to the period when the larvae stop eating and spinning.

[0026] Preferably, in step (1), the plant lactobacillus liquid is sprayed on fresh mulberry leaves for mild fermentation, which is more conducive to improving the effect of improving sleep disorders. The specific operation is: fresh mulberry leaves are laid flat in a single layer, and then the strain concentration is about 10 8 ~10 9 CFU / mL of bacterial solution was evenly sprayed on the surface of the leaves, and the spraying amount of the bacterial solution was 8-10% of the mass of the fresh mulberry leaves.

[0027] Preferably, in step (1), the temperature of the mild fermentation is 32-35° C. and the time is 6-8 h.

[0028] Preferably, the fermentation auxiliary materials are red date powder and lily powder, wherein the mass ratio of red date powder to lily powder is 3 to 5: 1 to 2. Lily and red date are plant raw materials that have the function of regulating sleep. When mixed with silkworm pupa powder as carbon sources, on the one hand, they ensure the smooth progress of fermentation, and on the other hand, they can convert polysaccharides in lily and red date into oligosaccharides, so that the silkworm pupa solid fermentation product is rich in lily oligosaccharides and red date oligosaccharides, which has a good effect on improving sleep disorders and improves the edible flavor quality of the fermentation product.

[0029] Preferably, in step (3), the amount of the fermentation auxiliary material added is 10-30% of the mass of the silkworm pupa powder; the mass ratio of the total amount of silkworm pupa powder + fermentation auxiliary material to water is 2-3:1-2, that is, the mass ratio of the fermentation mixture to water is 2-3:1-2.

[0030] Preferably, in step (3), after Lactobacillus rhamnosus and Pediococcus pentosaceus are mixed, they are inoculated into the fermentation mixture at a mass ratio of 0.2%, and fermented at 30-40°C for 3-5 days after inoculation; wherein the mass ratio of Lactobacillus rhamnosus to Pediococcus pentosaceus is 1-3:8-10.

[0031] Furthermore, in step (4), the fresh mulberry leaves are crushed and pulped, and then the obtained mulberry leaf pulp is inoculated with Aspergillus oryzae for fermentation, and after the fermentation is completed, freeze-dried to obtain a mulberry leaf fermentation product; and then the mulberry leaf fermentation product is subjected to extraction treatment to prepare a mulberry leaf extract C. Specifically, the present invention adds ethanol to the mulberry leaf fermentation product for extraction, and freeze-dries the supernatant to obtain a mulberry leaf extract A rich in hyperoside and isoquercetin; then the remaining mulberry leaf fermentation product residue is extracted with deionized water, and the supernatant is freeze-dried to obtain a mulberry leaf extract B rich in γ-aminobutyric acid; and the mulberry leaf extract A and the mulberry leaf extract B are mixed to obtain a mulberry leaf extract C rich in γ-aminobutyric acid, hyperoside and isoquercetin.

[0032] The present invention utilizes Aspergillus oryzae to ferment mulberry leaves, so that some of the gamma-aminobutyric acid, hyperoside and isoquercetin bound in the cell walls are released, and more gamma-aminobutyric acid, hyperoside and isoquercetin can be extracted in the subsequent extraction process, thereby obtaining the mulberry leaf extract C rich in gamma-aminobutyric acid, hyperoside and isoquercetin, and further improving the effect of improving sleep disorders.

[0033] Preferably, in step (4), Aspergillus oryzae is inoculated into the mulberry leaf pulp at a mass ratio of 0.2%, and fermented at 25-35°C for 2-5 days.

[0034] Preferably, in step (4), 75% ethanol solution is added to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 60 minutes; the supernatant is then concentrated, and ethanol is added to the solution to a final ethanol concentration of 80%, and the polysaccharides therein are precipitated and separated; the supernatant is freeze-dried to obtain the mulberry leaf extract A; deionized water is added to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 2 to 3 hours, and the supernatant is freeze-dried to obtain the mulberry leaf extract B; and mulberry leaf extract A and mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

[0035] Preferably, in step (5), the silkworm pupa solid-state fermentation product and mulberry leaf extract C are uniformly mixed in a ratio of 15-20:3-5 to obtain the silkworm and mulberry nutritional food base.

[0036] The present invention compounds animal-derived active peptides capable of improving sleep disorders with plant-derived extracts, so that the obtained silkworm and mulberry nutritional food base material has the characteristics of good effect in improving sleep disorders and good flavor.

[0037] To achieve the second object of the present invention, the present invention provides a sericulture nutritional food base material that helps improve sleep and is prepared by the above preparation method.

[0038] To achieve the third object of the present invention, the present invention provides the use of the above-mentioned silkworm nutrient food base in the preparation of functional food for assisting in improving sleep disorders.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) The present invention uses lightly fermented mulberry leaves obtained by fermentation with Lactobacillus plantarum, Bacillus subtilis or Leuconostoc mesenteroides to feed five-instar silkworms to obtain high-quality silkworm pupae, so that the silkworm nutrient food base prepared by the lightly fermented mulberry leaves has a better effect of improving sleep disorders than ordinary silkworm pupae; and the present invention uses Lactobacillus rhamnosus and Pediococcus pentosaceus to ferment silkworm pupae and hydrolyze silkworm pupa protein into small molecule peptides, so that the effect of improving sleep disorders of the silkworm nutrient food base is improved, and its edible flavor quality is improved, while reducing the allergenicity caused by silkworm pupae;

[0041] Furthermore, the present invention obtains mulberry leaf extracts rich in hyperoside, isoquercetin and γ-aminobutyric acid through two-step extraction, and the combination of the mulberry leaf extracts and the silkworm pupa solid-state fermentation product synergistically enhances the effect of improving sleep disorders.

[0042] (2) The present invention uses lily and red dates as fermentation auxiliary materials. First, the two raw materials have the function of regulating sleep and produce abundant lily oligosaccharides and red date oligosaccharides after fermentation. Second, the two raw materials are rich in functional carbohydrates, which can provide the carbon source required for fermentation and improve the fermentation efficiency. Therefore, the addition of the two raw materials can further enhance the effect of the sericulture nutritional food base material in improving sleep disorders.

[0043] (3) The present invention utilizes Aspergillus oryzae fermentation to further increase the contents of γ-aminobutyric acid, hyperoside and isoquercetin in the mulberry leaf extract, thereby further enhancing the effect of improving sleep disorders. DETAILED DESCRIPTION

[0044] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Example 1

[0046] The method for preparing a sericulture nutritious food base material that helps improve sleep comprises the following steps:

[0047] (1) Preparation of mulberry leaves for silkworm rearing: Lay out fresh mulberry leaves in a single layer and add a strain concentration of about 10 9 CFU / mL of Lactobacillus plantarum liquid was evenly sprayed on the surface of the mulberry leaves, and the spraying amount was 10% of the mass of the fresh mulberry leaves; then the mulberry leaves were placed in a fermentation tank and lightly fermented at 32°C for 6 hours without oxygen, and the surface moisture was dried after being taken out to obtain lightly fermented mulberry leaves;

[0048] (2) Preparation of silkworm pupae: From the first day of the fifth instar of silkworm larvae until they stop feeding and spinning silk, each silkworm eats 23 to 25 g of mulberry leaves in total. They are fed mulberry leaves three times a day, including one lightly fermented mulberry leaf feed and two normal fresh mulberry leaf feeds. That is, the total amount of lightly fermented mulberry leaves fed to each silkworm accounts for 1 / 3 of the total amount of mulberry leaves eaten. Specifically, the total amount of lightly fermented mulberry leaves eaten by each silkworm is 7.6 to 8.3 g.

[0049] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0050] (3) Preparation of silkworm pupa solid fermentation product: adding 12% of fermentation auxiliary materials to silkworm pupa powder, wherein the fermentation auxiliary materials are red date powder and lily powder (the mass ratio of the two is 5:1), adding water at a material-water mass ratio of 1:1, and mixing evenly to obtain a fermentation mixture; then, inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus at a mass ratio of 0.2% (the mass ratio of Lactobacillus rhamnosus and Pediococcus pentosaceus is 3:10), and fermenting at 30° C. for 3 days; after the fermentation is completed, freeze-drying is performed to obtain a silkworm pupa solid fermentation product;

[0051] (4) Preparation of mulberry leaf extract: crush and pulp fresh mulberry leaves, then inoculate Aspergillus oryzae into the mulberry leaf pulp at a mass ratio of 0.2%, and ferment at 25° C. for 2 days; freeze-dry after the fermentation to obtain a mulberry leaf fermentation product;

[0052] Adding 75% ethanol solution to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, extracting at 60°C for 60 minutes; then concentrating the supernatant, adding ethanol to a final ethanol concentration of 80% in the solution, precipitating and separating the polysaccharides therein; taking the supernatant and freeze-drying it to obtain the mulberry leaf extract A;

[0053] Add deionized water to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, extract at 60° C. for 2 h, and freeze-dry the supernatant to obtain the mulberry leaf extract B;

[0054] Combining mulberry leaf extract A and mulberry leaf extract B to obtain the mulberry leaf extract C;

[0055] (5) Preparation of sericulture nutritional food base: The silkworm pupa solid fermentation product obtained in step (3) and the mulberry leaf extract C obtained in step (4) are mixed evenly in a ratio of 5:1 to obtain the sericulture nutritional food base.

[0056] Example 2

[0057] The method for preparing a sericulture nutritious food base material that helps improve sleep comprises the following steps:

[0058] (1) Preparation of mulberry leaves for silkworm rearing: Lay out fresh mulberry leaves in a single layer and add a strain concentration of about 10 9 CFU / mL of plant lactobacillus liquid was evenly sprayed on the surface of the mulberry leaves, and the spraying amount was 10% of the mass of the fresh mulberry leaves; then the mulberry leaves were placed in a fermentation tank and lightly fermented at 35°C for 6 hours without oxygen, and the surface moisture was dried after being taken out to obtain lightly fermented mulberry leaves;

[0059] (2) Preparation of silkworm pupae: From the first day of the fifth instar of silkworm larvae until they stop feeding and spinning silk, each silkworm eats 23-25 ​​g of mulberry leaves in total; they are fed leaves three times a day, including two times of lightly fermented mulberry leaves and one time of normal fresh mulberry leaves. That is, the total amount of lightly fermented mulberry leaves fed to each silkworm accounts for 2 / 3 of the total amount of mulberry leaves eaten, which is 15.3-16.7 g.

[0060] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0061] (3) Preparation of silkworm pupa solid fermentation product: adding 15% of fermentation auxiliary materials to silkworm pupa powder, wherein the fermentation auxiliary materials are red date powder and lily powder (the mass ratio of the two is 3:1), adding water at a material-water mass ratio of 2:1, and mixing evenly to obtain a fermentation mixture; then, inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus at a mass ratio of 0.2% (the mass ratio of Lactobacillus rhamnosus and Pediococcus pentosaceus is 2:5), and fermenting at 35° C. for 3 days; after the fermentation is completed, freeze-drying is performed to obtain a silkworm pupa solid fermentation product;

[0062] (4) Preparation of mulberry leaf extract: crush and pulp fresh mulberry leaves, then inoculate Aspergillus oryzae into the mulberry leaf pulp at a mass ratio of 0.2%, and ferment at 32° C. for 3 days; freeze-dry after the fermentation to obtain a mulberry leaf fermentation product;

[0063] Adding 75% ethanol solution to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, extracting at 60°C for 60 minutes; then concentrating the supernatant, adding ethanol to a final ethanol concentration of 80% in the solution, precipitating and separating the polysaccharides therein; taking the supernatant and freeze-drying it to obtain the mulberry leaf extract A;

[0064] Add deionized water to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, extract at 60° C. for 2 h, and freeze-dry the supernatant to obtain the mulberry leaf extract B;

[0065] The mulberry leaf extract A and the mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

[0066] (5) Preparation of sericulture nutritional food base: The silkworm pupa solid fermentation product obtained in step (3) and the mulberry leaf extract C obtained in step (4) are mixed evenly in a ratio of 20:3 to obtain the sericulture nutritional food base.

[0067] Example 3

[0068] The method for preparing a sericulture nutritious food base material that helps improve sleep comprises the following steps:

[0069] (1) Preparation of mulberry leaves for silkworm rearing: Lay out fresh mulberry leaves in a single layer and add a strain concentration of about 10 9 CFU / mL of Lactobacillus plantarum liquid was evenly sprayed on the surface of the mulberry leaves, and the spraying amount was 10% of the mass of the fresh mulberry leaves; then the mulberry leaves were placed in a fermentation tank and lightly fermented at 32°C for 6 hours without oxygen, and the surface moisture was dried after being taken out to obtain lightly fermented mulberry leaves;

[0070] (2) Preparation of silkworm pupae: From the first day of the fifth instar of silkworm larvae until they stop feeding and spinning silk, each silkworm eats 23 to 25 g of mulberry leaves in total. They are fed mulberry leaves three times a day, including one lightly fermented mulberry leaf feed and two normal fresh mulberry leaf feeds. That is, the total amount of lightly fermented mulberry leaves fed to each silkworm accounts for 1 / 3 of the total amount of mulberry leaves eaten. Specifically, the total amount of lightly fermented mulberry leaves eaten by each silkworm is 7.6 to 8.3 g.

[0071] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0072] (3) Preparation of silkworm pupa solid fermentation product: adding 12% of fermentation auxiliary materials to silkworm pupa powder, wherein the fermentation auxiliary materials are red date powder and lily powder (the mass ratio of the two is 5:1), adding water at a material-water mass ratio of 1:1, and mixing evenly to obtain a fermentation mixture; then, inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus at a mass ratio of 0.2% (the mass ratio of Lactobacillus rhamnosus and Pediococcus pentosaceus is 3:10), and fermenting at 30° C. for 3 days; after the fermentation is completed, freeze-drying is performed to obtain a silkworm pupa solid fermentation product;

[0073] (4) Preparation of mulberry leaf extract: fresh mulberry leaves are crushed and pulped, and then freeze-dried to obtain mulberry leaf powder; 75% ethanol solution is added to the mulberry leaf powder at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 60 minutes; the supernatant is then concentrated, and ethanol is added to the solution to a final ethanol concentration of 80%, and the polysaccharides therein are precipitated and separated; the supernatant is freeze-dried to obtain the mulberry leaf extract A; deionized water is added to the remaining mulberry leaf powder residue at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 2 hours, and the supernatant is freeze-dried to obtain the mulberry leaf extract B; mulberry leaf extract A and mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

[0074] (5) Preparation of sericulture nutritional food base: The silkworm pupa solid fermentation product obtained in step (3) and the mulberry leaf extract C obtained in step (4) are mixed evenly in a ratio of 5:1 to obtain the sericulture nutritional food base.

[0075] Example 4

[0076] The method for preparing a sericulture nutritious food base material that helps improve sleep comprises the following steps:

[0077] (1) Preparation of mulberry leaves for silkworm rearing: Lay out fresh mulberry leaves in a single layer and add a strain concentration of about 10 9 CFU / mL of plant lactobacillus liquid was evenly sprayed on the surface of the mulberry leaves, and the spraying amount was 10% of the mass of the fresh mulberry leaves; then the mulberry leaves were placed in a fermentation tank and lightly fermented at 33°C for 6 hours without oxygen, and the surface moisture was dried after being taken out to obtain lightly fermented mulberry leaves;

[0078] (2) Preparation of silkworm pupae: From the first day of the fifth instar of silkworm larvae until they stop feeding and spinning silk, each silkworm eats 23 to 25 g of mulberry leaves in total; they are fed a mixture of lightly fermented mulberry leaves and ordinary fresh mulberry leaves every day, with the total amount of lightly fermented mulberry leaves fed to each silkworm accounting for 2 / 3 of the total amount of mulberry leaves eaten, which is 15.3 to 16.7 g;

[0079] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0080] (3) Preparation of silkworm pupa solid fermentation product: adding 12% of fermentation auxiliary materials to silkworm pupa powder, wherein the fermentation auxiliary materials are red date powder and lily powder (the mass ratio of the two is 5:1), adding water at a material-water mass ratio of 3:2, and mixing evenly to obtain a fermentation mixture; then, inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus at a mass ratio of 0.2% (the mass ratio of Lactobacillus rhamnosus and Pediococcus pentosaceus is 1:8), and fermenting at 30° C. for 3 days; after the fermentation is completed, freeze-drying is performed to obtain a silkworm pupa solid fermentation product;

[0081] (4) Preparation of mulberry leaf extract: crush and pulp fresh mulberry leaves, then inoculate Aspergillus oryzae into the mulberry leaf pulp at a mass ratio of 0.2%, and ferment at 25° C. for 2 days; freeze-dry after the fermentation to obtain a mulberry leaf fermentation product;

[0082] Adding 75% ethanol solution to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, extracting at 60°C for 60 minutes; then concentrating the supernatant, adding ethanol to a final ethanol concentration of 80% in the solution, precipitating and separating the polysaccharides therein; taking the supernatant and freeze-drying it to obtain the mulberry leaf extract A;

[0083] Deionized water was added to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, and the mixture was extracted at 60° C. for 2 h, and the supernatant was freeze-dried to obtain the mulberry leaf extract B;

[0084] The mulberry leaf extract A and the mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

[0085] (5) Preparation of sericulture nutritional food base: The silkworm pupa solid fermentation product obtained in step (3) and the mulberry leaf extract C obtained in step (4) are mixed evenly in a ratio of 5:1 to obtain the sericulture nutritional food base.

[0086] Example 5

[0087] The method for preparing a silkworm pupa nutritional food base material that helps improve sleep comprises the following steps:

[0088] (1) Preparation of mulberry leaves for silkworm rearing: Lay out fresh mulberry leaves in a single layer and add a strain concentration of about 10 8 CFU / mL of plant lactobacillus liquid was evenly sprayed on the surface of the mulberry leaves, and the spraying amount was 8% of the mass of the fresh mulberry leaves; then the mulberry leaves were placed in a fermentation tank and lightly fermented at 32°C for 8 hours without oxygen, and the surface moisture was dried after being taken out to obtain lightly fermented mulberry leaves;

[0089] (2) Preparation of silkworm pupae: From the first day of the fifth instar of silkworm larvae to the time of stopping feeding and spinning silk, each silkworm eats 23-25 ​​g of mulberry leaves in total; the silkworm is fed mulberry leaves three times a day, including two times of lightly fermented mulberry leaves and one time of normal fresh mulberry leaves, i.e., the total amount of lightly fermented mulberry leaves fed to each silkworm accounts for 2 / 3 of the total amount of mulberry leaves eaten, specifically, the total amount of lightly fermented mulberry leaves eaten by each silkworm is 15.3-16.7 g;

[0090] Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid;

[0091] (3) Preparation of silkworm pupa solid fermentation product: adding 18% fermentation auxiliary materials to silkworm pupa powder, wherein the fermentation auxiliary materials are red date powder and lily powder (the mass ratio of the two is 5:2), adding water at a material-water mass ratio of 3:1, and mixing evenly to obtain a fermentation mixture; then, inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus at a mass ratio of 0.2% (the mass ratio of Lactobacillus rhamnosus and Pediococcus pentosaceus is 2:5), and fermenting at 38° C. for 5 days; after the fermentation is completed, freeze-drying is performed to obtain a silkworm pupa solid fermentation product;

[0092] (4) Preparation of mulberry leaf extract: crush and pulp fresh mulberry leaves, then inoculate Aspergillus oryzae into the mulberry leaf pulp at a mass ratio of 0.2%, and ferment at 25° C. for 2 days; after the fermentation, freeze and crush to obtain a mulberry leaf fermentation product;

[0093] Adding 75% ethanol solution to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, extracting at 60°C for 60 minutes; then concentrating the supernatant, adding ethanol to a final ethanol concentration of 80% in the solution, precipitating and separating the polysaccharides therein; taking the supernatant and freeze-drying it to obtain the mulberry leaf extract A;

[0094] Deionized water was added to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, and the mixture was extracted at 60° C. for 2 h, and the supernatant was freeze-dried to obtain the mulberry leaf extract B;

[0095] The mulberry leaf extract A and the mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

[0096] (5) Preparation of sericulture nutritional food base: The silkworm pupa solid fermentation product powder obtained in step (3) and the mulberry leaf extract C obtained in step (4) are mixed evenly in a ratio of 5:1 to obtain the sericulture nutritional food base.

[0097] Example 6

[0098] The pharmacodynamic test of the silkworm nutritious food base material of the present invention on sleep regulation of sleep disorder rats is carried out by the following method:

[0099] 1. Sleep deprivation rat model

[0100] Fifteen narrow platforms were placed in a water tank filled with water, with the water level slightly below the top of the platform. The test rats were placed randomly on the top of the platform and allowed to jump from one platform to another. Once the rats fell asleep, they lost muscle tone and fell into the water to be awakened, and then continued to return to the platform. The experimental animals were separated into groups and each group was placed in a deprivation box. The water tank was filled with water until it was 1 cm away from the platform. The rats had free access to the clean water bottle and food supply basket hanging from the top. The rat sleep deprivation experiment was carried out for 5 consecutive days. During the sleep deprivation period, the temperature and light / dark cycle were kept under controlled conditions (20±2℃, 12:12h light-dark cycle).

[0101] 2. Morris water maze test

[0102] The water maze test consists of two experimental phases: localization navigation and spatial exploration. A large circular pool filled with water (opaque due to the addition of black ink) is divided into four quadrants. The escape platform is submerged 0.5 to 1.0 cm below the water surface in the northeast quadrant of the pool. During the training period, each rat is placed in the water at the designated starting point in different quadrants of the maze facing the pool wall. Each rat is trained with the same starting point. If the rat can successfully find the escape platform, the swimming time of the rat to find the platform before is defined and recorded as the escape latency. If and only if the rat fails to successfully reach the platform after 2 minutes, its escape latency is recorded as 2 minutes. After the localization navigation test is completed, the platform is removed for a spatial exploration test. The animal model is placed in the water along the pool wall at any starting point not close to the platform. The activity of the animal at the original position of the platform within 2 minutes is recorded.

[0103] 3. Animal grouping

[0104] According to the experimental design, 10 rats were randomly divided into seven groups: (1) normal control group (normal rats were treated with physiological saline, and the gavage volume was the same as that of the sample group); (2) model group (normal rats deprived of sleep); (3)-(7) sample group, Example 1-5 group (sleep-deprived rats were treated with the silkworm nutritious food base material samples prepared in Example 1 to 5 groups, and the dose was 600 mg / kg body weight), and the rats were treated with gavage for 4 weeks. The results are shown in Table 1.

[0105] Table 1 Effects of samples on water maze latency in sleep disorder model rats

[0106]

[0107] Note: The items without the same letter in the same column are significantly different, P < 0.05

[0108] Example 7

[0109] In the efficacy test of regulating sleep by feeding silkworms with different strains of bacteria, the following experimental groups and control groups were set up:

[0110] Test group 1: the sericulture nutritional food base prepared in Example 1;

[0111] Experimental group 2: The silkworm nutritious food base material was prepared by replacing Lactobacillus plantarum with Bacillus subtilis, and the other operations were the same as those in Example 1;

[0112] Experimental group 3: The silkworm nutritious food base material was prepared by replacing Lactobacillus plantarum with Leuconostoc mesenteroides, and the other operations were the same as those in Example 1;

[0113] Comparative group: the silkworm pupae raw material was obtained by feeding with ordinary fresh mulberry leaves, that is, ordinary fresh mulberry leaves were used to replace the silkworm nutritious food base prepared by lightly fermented mulberry leaves, and other operations were the same as in Example 1;

[0114] The test method is:

[0115] 1. Sleep deprivation rat model

[0116] Fifteen narrow platforms were placed in a water tank filled with water, with the water level slightly below the top of the platform. The test rats were placed randomly on the top of the platform and allowed to jump from one platform to another. Once the rats fell asleep, they lost muscle tone and fell into the water to be awakened, and then continued to return to the platform. The experimental animals were separated into groups and each group was placed in a deprivation box. The water tank was filled with water until it was 1 cm away from the platform. The rats had free access to the clean water bottle and food supply basket hanging from the top. The rat sleep deprivation experiment was carried out for 5 consecutive days. During the sleep deprivation period, the temperature and light / dark cycle were kept under controlled conditions (20±2℃, 12:12h light-dark cycle).

[0117] 2. Morris water maze test

[0118] The water maze test consists of two experimental phases: localization navigation and spatial exploration. A large circular pool filled with water (opaque due to the addition of black ink) is divided into four quadrants. The escape platform is submerged 0.5 to 1.0 cm below the water surface in the northeast quadrant of the pool. During the training period, each rat is placed in the water at the designated starting point in different quadrants of the maze facing the pool wall. Each rat is trained with the same starting point. If the rat can successfully find the escape platform, the swimming time of the rat to find the platform before is defined and recorded as the escape latency. If and only if the rat fails to successfully reach the platform after 2 minutes, its escape latency is recorded as 2 minutes. After the localization navigation test is completed, the platform is removed for a spatial exploration test. The animal model is placed in the water along the pool wall at any starting point not close to the platform. The activity of the animal at the original position of the platform within 2 minutes is recorded.

[0119] 3. Animal grouping

[0120] According to the experimental design, each group of 10 rats was randomly divided into six groups: (1) normal control group (normal rats were treated with physiological saline, and the gavage volume was the same as that of the sample group); (2) model group (normal rats deprived of sleep); (3)-(6) sample groups, i.e., test groups 1-3 and control group (sleep-deprived rats were treated with corresponding silkworm nutritious food base samples by gavage, with a dose of 600 mg / kg body weight), and the rats were treated by gavage for 4 weeks. The results are shown in Table 2.

[0121] Table 2 Effects of samples on water maze latency in sleep disorder model rats

[0122]

[0123] Note: Different lowercase letters in the table indicate significant differences between the two at the 0.05 level.

[0124] Example 8

[0125] The efficacy comparison test of mulberry leaf extracts with different treatments on regulating sleep was conducted, and the following experimental and control groups were set up:

[0126] Test group: the sericulture nutritional food base prepared in Example 1;

[0127] Control group: mulberry leaf extract was prepared by directly extracting mulberry leaf fermentation products with deionized water, omitting the step of ethanol extraction. The remaining steps were the same as those in Example 1 to prepare the sericulture nutritional food base.

[0128] The test method is:

[0129] 1. Sleep deprivation rat model

[0130] Fifteen narrow platforms were placed in a water tank filled with water, with the water level slightly below the top of the platform. The test rats were placed randomly on the top of the platform and allowed to jump from one platform to another. Once the rats fell asleep, they lost muscle tone and fell into the water to be awakened, and then continued to return to the platform. The experimental animals were separated into groups and each group was placed in a deprivation box. The water tank was filled with water until it was 1 cm away from the platform. The rats had free access to the clean water bottle and food supply basket hanging from the top. The rat sleep deprivation experiment was carried out for 5 consecutive days. During the sleep deprivation period, the temperature and light / dark cycle were kept under controlled conditions (20±2℃, 12:12h light-dark cycle).

[0131] 2. Morris water maze test

[0132] The water maze test consists of two experimental phases: localization navigation and spatial exploration. A large circular pool filled with water (opaque due to the addition of black ink) is divided into four quadrants. The escape platform is submerged 0.5 to 1.0 cm below the water surface in the northeast quadrant of the pool. During the training period, each rat is placed in the water at the designated starting point in different quadrants of the maze facing the pool wall. Each rat is trained with the same starting point. If the rat can successfully find the escape platform, the swimming time of the rat to find the platform before is defined and recorded as the escape latency. If and only if the rat fails to successfully reach the platform after 2 minutes, its escape latency is recorded as 2 minutes. After the localization navigation test is completed, the platform is removed for a spatial exploration test. The animal model is placed in the water along the pool wall at any starting point not close to the platform. The activity of the animal at the original position of the platform within 2 minutes is recorded.

[0133] 3. Animal grouping

[0134] According to the experimental design, 10 rats were randomly divided into four groups: (1) normal control group (normal rats were treated with physiological saline, and the gavage volume was the same as that of the sample group); (2) model group (normal rats deprived of sleep); (3)-(4) sample groups, i.e., experimental group and control group (sleep-deprived rats were treated with corresponding silkworm nutritious food base samples by gavage, with a dose of 600 mg / kg body weight), and the rats were treated by gavage for 4 weeks. The results are shown in Table 3.

[0135] Table 3 Effects of samples on water maze latency in sleep disorder model rats

[0136]

[0137] Note: Different lowercase letters in the table indicate significant differences between the two at the 0.05 level.

[0138] Example 9

[0139] The experiment to verify the sleep regulating effect of the combination of silkworm pupa solid fermentation product and mulberry leaf extract C set up the following experimental group and control group:

[0140] Test group: the sericulture nutritional food base prepared in Example 1;

[0141] Comparative group 1: the solid-state fermentation product of silkworm pupae prepared in step (3) of Example 1;

[0142] Comparative Group 2: Mulberry leaf extract C prepared in step (4) of Example 1.

[0143] The test method is:

[0144] 1. Sleep deprivation rat model

[0145] Fifteen narrow platforms were placed in a water tank filled with water, with the water level slightly below the top of the platform. The test rats were placed randomly on the top of the platform and allowed to jump from one platform to another. Once the rats fell asleep, they lost muscle tone and fell into the water to be awakened, and then continued to return to the platform. The experimental animals were separated into groups and each group was placed in a deprivation box. The water tank was filled with water until it was 1 cm away from the platform. The rats had free access to the clean water bottle and food supply basket hanging from the top. The rat sleep deprivation experiment was carried out for 5 consecutive days. During the sleep deprivation period, the temperature and light / dark cycle were kept under controlled conditions (20±2℃, 12:12h light-dark cycle).

[0146] 2. Morris water maze test

[0147] The water maze test consists of two experimental phases: localization navigation and spatial exploration. A large circular pool filled with water (opaque due to the addition of black ink) is divided into four quadrants. The escape platform is submerged 0.5 to 1.0 cm below the water surface in the northeast quadrant of the pool. During the training period, each rat is placed in the water at the designated starting point in different quadrants of the maze facing the pool wall. Each rat is trained with the same starting point. If the rat can successfully find the escape platform, the swimming time of the rat to find the platform before is defined and recorded as the escape latency. If and only if the rat fails to successfully reach the platform after 2 minutes, its escape latency is recorded as 2 minutes. After the localization navigation test is completed, the platform is removed for a spatial exploration test. The animal model is placed in the water along the pool wall at any starting point not close to the platform. The activity of the animal at the original position of the platform within 2 minutes is recorded.

[0148] 3. Animal grouping

[0149] According to the experimental design, 10 rats were randomly divided into five groups: (1) normal control group (normal rats were treated with normal saline, and the gavage volume was the same as that of the sample group); (2) model group (normal rats deprived of sleep); (3)-(5) sample groups, i.e., test group and control group 1-2 (sleep-deprived rats were treated with corresponding samples by gavage, with a dose of 600 mg / kg body weight), and the rats were treated by gavage for 4 weeks. The results are shown in Table 4.

[0150] Table 4 Effects of samples on water maze latency in sleep disorder model rats

[0151]

[0152] Note: Different lowercase letters in the table indicate significant differences between the two at the 0.05 level.

[0153] The results in Table 4 show that the silkworm pupa solid-state fermentation product prepared by the present invention and the mulberry leaf extract C rich in hyperoside, isoquercetin and γ-aminobutyric acid synergistically enhance the effect of improving sleep disorders.

Claims

1. A method for preparing a sericulture nutritional food base material that helps improve sleep, characterized in that: The following steps are involved: (1) preparing mulberry leaves for silkworm breeding: selecting one of Lactobacillus plantarum, Bacillus subtilis and Leuconostoc mesenteroides and spraying it evenly on fresh mulberry leaves, then placing the mulberry leaves in a fermentation tank for short-term, anaerobic and mild fermentation, taking them out and drying the surface moisture to obtain mildly fermented mulberry leaves; (2) Preparation of silkworm pupae: From the first day of the fifth instar of the silkworm larvae until they stop eating and spinning silk, the silkworm larvae are fed with the lightly fermented mulberry leaves 1 to 2 times a day, and are fed with ordinary fresh mulberry leaves normally during the rest of the time, or the lightly fermented mulberry leaves are used to replace part of the ordinary fresh mulberry leaves for feeding every day; Then, fresh silkworm pupae after spinning silk and forming cocoons are freeze-dried and crushed to obtain silkworm pupae powder rich in γ-aminobutyric acid; (3) Preparation of silkworm pupa solid fermentation product: mixing silkworm pupa powder with fermentation auxiliary materials and water to obtain a fermentation mixture; then inoculating the fermentation mixture with Lactobacillus rhamnosus and Pediococcus pentosaceus to ferment; and freeze-drying after the fermentation to obtain a silkworm pupa solid fermentation product; (4) Preparation of mulberry leaf extract: crushing and pulping fresh mulberry leaves, and then freeze-drying to obtain mulberry leaf powder; adding ethanol to the mulberry leaf powder for extraction, and freeze-drying the supernatant to obtain a mulberry leaf extract A rich in hyperoside and isoquercetin; The remaining mulberry leaf powder residue is then extracted with deionized water, and the supernatant is freeze-dried to obtain a mulberry leaf extract B rich in gamma-aminobutyric acid; Mixing mulberry leaf extract A and mulberry leaf extract B to obtain mulberry leaf extract C rich in gamma-aminobutyric acid, hyperoside and isoquercetin; (5) Preparation of sericulture nutritional food base: The silkworm pupae solid fermentation product and mulberry leaf extract C are mixed evenly to obtain the sericulture nutritional food base.

2. The method for preparing the sericulture nutritional food base material according to claim 1, characterized in that: In step (1), a Lactobacillus plantarum liquid is selected and sprayed onto fresh mulberry leaves; the specific operation is: fresh mulberry leaves are laid flat in a single layer, and then the concentration of the strain is about 10 8 ~10 9 The bacterial solution with CFU / mL is evenly sprayed on the surface of the mulberry leaves, and the spraying amount of the bacterial solution is 8-10% of the mass of the fresh mulberry leaves; the temperature of the mild fermentation is 32-35°C, and the time is 6-8h.

3. The method for preparing the sericulture nutritional food base material according to claim 2, characterized in that: The fermentation auxiliary materials are red date powder and lily powder, wherein the mass ratio of red date powder to lily powder is 3-5:1-2; the added amount of the fermentation auxiliary materials is 10-30% of the mass of the silkworm pupa powder, and the mass ratio of the total amount of silkworm pupa powder + fermentation auxiliary materials to water is 2-3:1-2.

4. The method for preparing the sericulture nutritional food base material according to claim 3, characterized in that: In step (3), after Lactobacillus rhamnosus and Pediococcus pentosaceus are mixed, they are inoculated into the fermentation mixture at a mass ratio of 0.2%, and fermented at 30-40°C for 3-5 days; wherein the mass ratio of Lactobacillus rhamnosus to Pediococcus pentosaceus is 1-3:8-10.

5. The method for preparing the sericulture nutritional food base material according to claim 4, characterized in that: In step (4), fresh mulberry leaves are crushed and pulped, and then the obtained mulberry leaf pulp is inoculated with Aspergillus oryzae for fermentation, and freeze-dried after the fermentation is completed to obtain a mulberry leaf fermentation product; then ethanol is added to the mulberry leaf fermentation product for extraction, and the supernatant is freeze-dried to obtain a mulberry leaf extract A rich in hyperoside and isoquercetin; the remaining mulberry leaf fermentation product residue is then extracted with deionized water, and the supernatant is freeze-dried to obtain a mulberry leaf extract B rich in γ-aminobutyric acid; the mulberry leaf extract A and the mulberry leaf extract B are mixed to obtain a mulberry leaf extract C rich in γ-aminobutyric acid, hyperoside and isoquercetin.

6. The method for preparing the sericulture nutritional food base material according to claim 5, characterized in that: Aspergillus oryzae is inoculated into mulberry leaf pulp at a mass ratio of 0.2%, and fermented at 25-35°C for 2-5 days.

7. The method for preparing the sericulture nutritional food base material according to claim 6, characterized in that: In step (4), 75% ethanol solution is added to the mulberry leaf fermentation product at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 60 minutes; the supernatant is then concentrated, and ethanol is added to the solution to a final ethanol concentration of 80%, and the polysaccharides therein are precipitated and separated; the supernatant is freeze-dried to obtain the mulberry leaf extract A; deionized water is added to the remaining mulberry leaf fermentation product residue at a material-liquid mass ratio of 1:10, and the mixture is extracted at 60°C for 2 to 3 hours, and the supernatant is freeze-dried to obtain the mulberry leaf extract B; and the mulberry leaf extract A and the mulberry leaf extract B are combined to obtain the mulberry leaf extract C.

8. The method for preparing the sericulture nutritional food base material according to claim 7, characterized in that: In step (5), the silkworm pupa solid fermentation product and mulberry leaf extract C are uniformly mixed in a ratio of 15-20:3-5 to obtain the silkworm and mulberry nutritional food base.

9. A sericulture nutritional food base material that helps improve sleep and is prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the sericulture nutritional food base material according to claim 9 in preparing functional food for assisting in improving sleep disorders.

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