Pupa Cordyceps sinensis fruiting body using sulphur pupa as host and cultivating process thereof

A method for cultivating Tenebrio molitor and a technology for Tenebrio molitor, which are applied in the directions of fungi and the like, can solve problems such as the cultivation of pupae infected with Tenebrio molitor, and achieve the effects of simple cultivation method and high growth rate.

Active Publication Date: 2006-06-28
GUANGDONG INST OF MICROORGANISM
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AI Technical Summary

Problems solved by technology

Relying solely on natural resources cannot meet people's needs, therefore, strengthening the artificial cultivation of Cordyceps militaris is the best way to solve this c

Method used

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Experimental program
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Embodiment Construction

[0028] The present invention will be further described below in conjunction with specific implementation methods.

[0029] (1) Tenebrio molitor pupation:

[0030] The larvae of Tenebrio molitor (L.) were cultured indoors to obtain pupae, which were used to cultivate fruiting bodies of Cordyceps militaris.

[0031] (2) Strain preparation

[0032] (2.1) Bacteria: collect the fruiting bodies of Cordyceps militaris, obtain through separation and purification of polyspores, and the formula of separation medium: glucose 1%, peptone 1%, MgSO 4 ·7H 2 O0.05%, vitamin B1 a little, agar 1.5%, pH6.5. Through the weeding test, it conforms to the basic characteristics of Cordyceps militaris (L.: Fr.) Link, and the anamorph is Paecilomyces (Paecilomyces), see "Southwest Agricultural Journal" (Liang Zongqi. The anamorph and fruiting body of Cordyceps militaris Artificial cultivation research. Southwest Agricultural Journal, 1990 (2): 1-8).

[0033] (2.2) Class I strains: connect to a sol...

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Abstract

The invention provides a cordyceps militaris fruiting body culturing method used yellow meal worm pupae as host. The fruiting body is cultured by using cordyceps militaris link to infect yellow meal worm. Its features are as follows: yellow meal worm is yellow brown or chocolate brown; fruiting body is bar type and orange yellow. The culturing method includes bacteria species preparing, yellow meal worm inoculating infecting, and out grass. The formed product has multi nutrient content and effect active constituent, and uses as foods. The culturing method is simple, effective, and reliable. And the cost is low.

Description

technical field [0001] The invention relates to a fruiting body product of Cordyceps militaris and a cultivation method thereof in the field of biotechnology. Background technique [0002] Cordyceps militaris (L.: Fr.) Link, also known as Cordyceps militaris, belongs to the type species of Ascomycota, Ascomycetes, Hypocreales, Clavicipitaceae, and Cordyceps. Cordyceps militaris is a widely distributed species in the world, and it is distributed in all provinces of my country. From spring to autumn, it grows on the pupae of Lepidoptera insects half-buried on the forest floor or under the layer of decaying leaves. [0003] Cordyceps militaris and the same genus Cordyceps sinensis C.sinensis (Berk) Sacc. have very similar health effects and medicinal value, such as anti-fatigue, anti-aging, enhancing immunity, anti-inflammation, on S 180 tumors and other tumors have a strong inhibitory effect. Cordyceps militaris is favored by consumers for its rich nutritional ingredients an...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 李泰辉林群英宋斌黄浩钟月金沈亚恒
Owner GUANGDONG INST OF MICROORGANISM
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