Cultivation method of cordyceps militaris

A cultivation method and a technology for Cordyceps militaris, applied in the field of microorganisms, can solve problems such as low yield of cordycepin, and achieve the effects of efficient growth and reproduction

Active Publication Date: 2013-06-05
熊艳
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Problems solved by technology

[0006] But, the yield of the cordycepin obtained by the cultivation method of the

Method used

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  • Cultivation method of cordyceps militaris

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Embodiment Construction

[0011] The present invention will be described in further detail below through specific implementation examples and in conjunction with the accompanying drawings.

[0012] Please refer to figure 1 , the culture medium of the Cordyceps militaris that the embodiment of the present invention provides, comprises: Step 101: Prepare slant culture medium, liquid seed culture medium and fruiting body culture medium respectively; , and cultivated at 26-28°C for 6-8 days to obtain slant strains; step 103: transfer the slant strains to the liquid seed medium, shake at 26-28°C at 250 rpm bed culture for 2-3 days to obtain seed liquid; step 104: transfer the seed liquid to the sterilized fruit body cultivation medium, and place it in a sterilized light-proof cultivation room for dark cultivation to obtain Mycelium; step 105: carry out color-changing culture on the mycelium, and obtain mycelia with buds; step 106: carry out fruiting body culture on the mycelium with buds, and obtain Cordyc...

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Abstract

The invention relates to the field of microorganisms, in particular to a cultivation method of cordyceps militaris. The cultivation method can produce a large quantity of cordyceps militaris rhzomorph. The cultivation method comprises respectively preparing a slope culture medium, a liquid seed culture medium and a sporocarp cultivation culture medium; vaccinating cordyceps militaris cultures on the slope culture medium and cultivating at 23-25 DEG C for 6-8 days to obtain slope cultures; vaccinating the slope cultures on the liquid seed culture medium and conducting shake cultivation at 23-25 DEG C for 2-3 days at the speed of 250 circles/minute to obtain seed liquid; vaccinating the seed liquid on the sterilized sporocarp cultivation culture medium and conducting dark cultivation in a disinfected light-shielding culture room to obtain mycelium; conducting coloring cultivation on the mycelium and obtaining mycelium with buttons; and conducting sporocarp cultivation on the mycelium with the buttons to obtain the cordyceps militaris rhzomorph.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a cultivation method of Cordyceps militaris. Background technique [0002] Cordycepin is a nucleotide composed of adenosine and carbon-branched deoxypentose, so it is also called 3'-deoxyadenosine (3'-deoxyadenosine, 3'-dA), and its molecular formula is C 10 h 13 N 5 o 3 , the molecular weight is 251, the melting point is 230-231°C, and the maximum absorption wavelength of ultraviolet light is 259nm. [0003] Cordyceps militaris (L.) Link belongs to Fungi Kingdom (Fungi), Ascomycota Ascomycota, Ascomycetes Ascomycetes, Sordariomycetidae, Hypocreales, Clavicipitaceae, Cordyceps Cordyceps; Cordyceps is the main producer of cordycepin, its content of cordycepin is dozens of times that of Cordyceps sinensis, and it is the main source of cordycepin. [0004] At present, in related technologies, Cordyceps militaris strains are usually used as a source of culture and propagation to pr...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 熊艳
Owner 熊艳
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