Method for culturing cordyceps militaris by living silkworm chrysalises

A technology for Cordyceps militaris and silkworm chrysalis, which is applied to botanical equipment and methods, fertilizer mixtures, gardening and other directions, can solve the problems of low price, difficult to guarantee product quality, low grade and the like, and achieve the effect of increasing the content of active ingredients

Inactive Publication Date: 2010-06-09
江苏江南生物科技有限公司
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Problems solved by technology

[0003] At present, the artificial cultivation of Cordyceps militaris is mainly done by adding silkworm chrysalis powder to rice as the culture substrate. The grown Cordyceps militaris only has spores (grass) and no insect body, which does not match the name of Cordyceps militaris, and the

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Embodiment 1

[0018] 1. Preparation of liquid strain of Cordyceps militaris: prepare liquid strain culture liquid according to 20% of potato cooking liquid and 80% of nutrient solution, wherein the formula of nutrient solution is: peptone 8g, glucose 20g, ammonium citrate 1g, magnesium sulfate 1g, diphosphate Potassium Hydrogen 1g, Vitamin B 1 2 tablets, water 1000ml, pH 7.5. Put the prepared culture solution into a 500ml triangular flask, saline bottle or can bottle according to the quantity of 200-250ml per bottle, seal it with cotton or polypropylene film, put it into a medical portable pressure cooker for sterilization, 1.1-1.3 kg / cm 2 pressure, hold for 30-35 minutes. After the temperature of the culture solution is cooled to below 30°C, in a sterile environment, insert 2-3 pieces of Cordyceps militaris slanted parent species of rice grains with vigorous mycelium vitality into each bottle. Put the bottle connected with the strain into the strain cultivation room for shading and airt...

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Abstract

The invention belongs to the field of edible fungus culture and discloses a method for culturing cordyceps militaris by living silkworm chrysalises. The method comprises the following steps of: the preparation of stains, the selection of the silkworm chrysalises and disinfection treatment. The method is characterized by specifically comprising the following steps of: inoculating cordyceps militaris stains into silkworm chrysalis bodies through an injection method, wherein the stain dosage of each silkworm chrysalis body is 0.1-0.5ml; putting the inoculated silkworm chrysalises in sterile glass containers to culture the silkworm chrysalises in a culture room for 10-15 days at 18-25 DEG C, wherein 10-20 silkworm chrysalises are put in each container generally; increasing illumination from the twelfth day to promote sprouts to form; when little grass grows to 1cm, puncturing holes on sealing films so as to increase oxygen supply; meanwhile, watering the ground of the culture room to supplement the humidity; and after 20-30 days, when the height of stromata is 5-8 cm and the tops grow into wooden club shapes, collecting and drying. The method directly adopts the live silkworm chrysalises as culture carriers, can harvest the cordyceps militaris with the silkworm chrysalis bodies and changes the current situation that the cordyceps militaris cultured by rice culture media has no silkworm chrysalises.

Description

technical field [0001] The invention relates to a method for cultivating edible fungi, in particular to a method for cultivating Cordyceps militaris by using live silkworm chrysalis, and belongs to the technical field of edible fungus production. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a precious Chinese herbal medicine with high health care and nourishing value. Many medicinal ingredients contained in Cordyceps militaris, such as cordycepin, cordycepic acid, and polysaccharides, are close to or exceed those of Cordyceps sinensis, and have similar magical effects to Cordyceps sinensis. After the success of artificial cultivation of Cordyceps militaris, it has developed rapidly in recent years. The output of Cordyceps militaris has increased rapidly, the price has dropped significantly, and the market consumption has also increased accordingly. There is a trend of developing from medicine to food. In 2008, the Ministry of Heal...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 姜建新姜小红姜雅马一鸣
Owner 江苏江南生物科技有限公司
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