Method for producing cordycepin, breeding of high production cordyceps militaris link bacterial strain BYB-08 and application

A technology of BYB-08 and cordycepin, which is applied in the field of agricultural microorganisms, can solve the problems of undeclared content of cordycepin, no submission of biological material samples for preservation, and inability to evaluate the effect of the invention, etc.

Active Publication Date: 2008-08-20
武汉华裕新美菌业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent application is insufficiently disclosed as the document does not disclose the origin of the strains used for the fermentation, nor does it submit a deposit of samples of biological material for the patent procedure
The patent document for the production of cordycepin with the application number 01133167.7 relates to a liquid culture of cordycepin and a method for preparing cordycepin by using its culture fluid, but the specification of the application does not specify the content of cordycepin in the liquid medium, and people cannot evaluate The effect of its invention

Method used

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  • Method for producing cordycepin, breeding of high production cordyceps militaris link bacterial strain BYB-08 and application
  • Method for producing cordycepin, breeding of high production cordyceps militaris link bacterial strain BYB-08 and application
  • Method for producing cordycepin, breeding of high production cordyceps militaris link bacterial strain BYB-08 and application

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Embodiment 1

[0033] Embodiment 1: Breeding of Cordyceps militaris high-yield strain BYB-08

[0034] The strain used in the production process of the present invention is a high-yielding Cordyceps militaris strain selected by the Applied Fungi Research Institute of Huazhong Agricultural University where the applicant is located by using the inactivated protoplast fusion method, and the applicant named the strain BYB-08. The Cordyceps militaris strain (Cordyceps militaris) BYB-08 was sent to the Chinese Type Culture Collection Center in Wuhan University, Wuhan City, Hubei Province, China for preservation on June 29, 2007, and the accession number was: CCTCC NO: M207091. Specific steps are as follows:

[0035] (1) Screening of protoplast fusion parent strains

[0036] Nine Cordyceps militaris strains were collected from all over the country, numbered NO1-NO9 respectively. Prepare the following medium: (1) mycelium medium (recipe is as follows: glucose 20g, peptone 2g, yeast extract 20g, MgS...

Embodiment 2

[0049] Embodiment 2: The bacterial strain of the present invention is applied to the production example of cordycepin

[0050] (1) Strain preparation: solid culture medium was used to prepare strains (the strain is Cordyceps militaris BYB-08 selected and bred by the present invention, and the preservation number is CCTCC NO: M207091). The components and proportions of the medium are (according to weight and volume): 20 g of potato extract powder (purchased from commercial products), 20 g of glucose, 15 g of peptone, KH 2 PO 4 1g, MgSO 4 0.5g, VB 1 10mg, agar powder 20g, add tap water to 1000ml, adjust pH to 6.5-7.5. Sterilize by high-pressure steam at 121°C for 45 minutes to 1 hour according to conventional methods, and set aside.

[0051] Pour 20ml of the sterilized culture medium into each sterilized Petri dish, and after it solidifies and cools down, the access size is 1cm 2 The preservation number of the left and right is CCTCC NO: M207091 Cordyceps militaris strai...

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Abstract

The invention pertains to the field of agriculture microbial technology, in particular to a method for producing cordycepin, a breeding and an application of a high-yield Cordyceps militaris bacterial strain. The method obtains the high-yield Cordyceps militaris bacterial strain BYB-08 by mutation breeding, and the bacterial strain is collected in China Center for Type Culture Collection as a patent procedure before the application date (the collection number is CCTCC NO: M207091). The invention discloses a successful embodiment in a combined method of the optimized deep fermentation and the still placement surface layer fermentation culture of the Cordyceps militaris bacterial strain BYB-08 which is obtained by the method, compared with the cordycepin biological yield which is obtained by the methods which are reported in the existing patent documents and the non-patent documents, the cordycepin content in the obtained fermentation liquid of the invention is significantly improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms. It specifically relates to a production method of cordycepin and the breeding and application of the Cordyceps militaris strain used in the method. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris (abbreviated as Cordyceps militaris), silkworm caterpillar fungus, belongs to Ascomycotina (Ascomycotina), Sclerotinia (Pyrenomycetes), Sphaerinles (Sphaerinles), Ergotaceae (Claviticpitaceae), Cordyceps (Coedyceps) ), is one of the precious Chinese herbal medicines in my country. The anamorph of Cordyceps militaris is Paecilomyces militaris Liang, and its anamorph differs greatly in terms of colony characteristics, sexual fruiting body formation ability, and sporulation structure at the level of monospore strains. Cordyceps militaris and Cordyceps sinensis [Cordyceps sinensis (Berk.) Sacc.] are the model species of the fungus of the genus Cordyce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12N1/14C12R1/645
Inventor 边银丙周洪英俞海峰
Owner 武汉华裕新美菌业有限公司
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