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Method for extracting cordycepin and cordyceps polysaccharides from residue of cordyceps militaris culture medium

A technology for Cordyceps militaris and Cordyceps polysaccharide, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of high equipment requirements, high production costs, and many separation steps, and reduce investment and operating costs. , the effect of high content of active ingredients, simple process and equipment

Active Publication Date: 2010-12-08
唐培宸
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, according to the patent documents on the extraction of cordycepin and polysaccharides from Cordyceps militaris or its culture medium publicly reported at home and abroad, the extraction technology is a single component step-by-step extraction. Even in large-scale production, multiple sets of equipment systems are often required for discontinuous There are many extraction and separation steps, large investment and high production cost
For example, in some patents, ion exchange resins are used for step-by-step extraction. When the resin is regenerated, a large amount of chemical reagents such as acids and bases are required, which requires high equipment for large-scale production, and brings waste gas and waste water, causing environmental pollution.
In addition, some patents report the use of macroporous resins, but cordycepin and cordyceps polysaccharides cannot be extracted at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Put 250 grams of pulverized Cordyceps militaris culture medium residue particles into a φ5.0×40cm PVC pipe that can be sealed at both ends. The filling should be even and not squeezed, and the PVC pipe should be tightly closed with a lid.

[0038] Use a peristaltic pump to pump 2500ml of drinking water from the bottom of the PVC pipe, and the water extract from the upper end of the PVC pipe flows into the glass column (φ2.5×50cm) filled with 100ml of H-20 macroporous resin from the lower end, and the upper end of the glass column The effluent continues to be pumped into the bottom end of the PVC pipe with a peristaltic pump for water circulation extraction; the opening time of the peristaltic pump, that is, the extraction time, is 16 hours.

[0039] Use 400ml of 30% (V / V) ethanol aqueous solution as the eluent, and use a high-pressure diaphragm pump to pump the eluent into a glass column equipped with macroporous resin for elution at a flow rate of 1BV / h.

[0040] The e...

Embodiment 2

[0042]Put 250 grams of crushed Cordyceps militaris culture medium residue particles into a φ5.0×40cm PVC pipe that can be sealed at both ends, pump 500ml drinking water from the bottom end of the PVC pipe with a peristaltic pump, and the water coming out of the upper end of the PVC pipe The extraction liquid flows from the lower end into a glass column (φ2.5×50cm) filled with 100ml H-20 macroporous resin, and the effluent from the upper end of the glass column continues to be pumped into the bottom end of the PVC pipe with a peristaltic pump for water circulation extraction, and the peristaltic pump is turned on The time, namely the extraction time, was 16h.

[0043] Use 400ml of 10% (V / V) ethanol aqueous solution as the eluent, and use a high-pressure diaphragm pump to pump the eluent into a glass column equipped with macroporous resin for elution at a flow rate of 1BV / h.

[0044] The eluent was concentrated under reduced pressure with a rotary evaporator, and the concentrati...

Embodiment 3

[0046] Put 24.32 kg of pulverized Cordyceps militaris culture medium residue particles into a φ30×60cm stainless steel barrel that can be sealed at both ends. The filling should be even and not squeezed, and the barrel should be tightly closed with a lid.

[0047] Use a high-pressure diaphragm pump to pump 50 liters of drinking water from the bottom of the stainless steel barrel, and the water extract from the upper end of the stainless steel barrel flows into a stainless steel column (φ10×120cm) filled with 9 liters of H-20 macroporous resin from the lower end. The stainless steel column The effluent from the upper end continues to be pumped into the bottom of the stainless steel barrel with a high-pressure diaphragm pump for water circulation extraction. The opening time of the high-pressure diaphragm pump, that is, the extraction time, is 16 hours.

[0048] Use 30 liters of 20% (V / V) ethanol aqueous solution as the eluent, and use a high-pressure diaphragm pump to pump the e...

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Abstract

The invention discloses a method for simultaneously extracting cordycepin and cordyceps polysaccharides from a residue of a cordyceps militaris culture medium. The method comprises the following steps of: extracting cordycepin and cordyceps polysaccharides in a residue of a cordyceps militaris culture medium by utilizing an extractant, meanwhile, adsorbing the extracting solution through macroporous resin, and then extracting and adsorbing the residue of the cordyceps militaris culture medium; eluting the cordycepin and the cordyceps polysaccharides, which are adsorbed on the macroporous resin; and concentrating and drying the eluent to obtain a product containing the cordycepin and the cordyceps polysaccharides. The method can simultaneously extract the cordycepin and the cordyceps polysaccharides from the residue of the cordyceps militaris culture medium and has high active constituent content; the resin does not need acid-alkali regeneration and can be recycled; and the method has the advantages of environmental protection, low cost, simple process and equipment and realization of continuous extraction.

Description

technical field [0001] The invention relates to the field of medicine and chemical industry, in particular to a method for extracting cordycepin and cordyceps polysaccharide from the culture medium residue of Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to Ascomycotina, Ergotaceae, Cordyceps, and is a precious medicinal fungus. In recent years, due to the increasing demand for natural Cordyceps sinensis and the depletion of limited resources, it has not yet been artificially cultivated on a large scale. So people began to cultivate Cordyceps militaris with a short production cycle and similar medical and health care effects with natural Cordyceps sinensis to meet consumer demand, replace the shortage of natural Cordyceps sinensis, and provide a broad market and good development opportunities for the development of artificial large-scale cultivation of Cordyceps militaris. [0003] Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 唐永范唐亮
Owner 唐培宸
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