Cordyceps militaris fruiting body with silkworm larvae as hosts and cultivation method of cordyceps militaris fruiting body

Abstract

The invention relates to a cordyceps militaris fruiting body with silkworm larvae as hosts and a cultivation method of the cordyceps militaris fruiting body. The cultivation method includes the steps: disinfecting and washing the cordyceps militaris fruiting body, cutting the cordyceps militaris fruiting body into sections, and inoculating the sections to a solid culture medium for cultivation to obtain a bacterial mass; inoculating the bacterial mass to a primary liquid strain culture medium for cultivation to obtain a primary liquid strain; diluting the primary liquid strain with sterile water to obtain primary liquid strain diluent; mixing pearlite and vermiculite, mixing the disinfected mixture with the primary liquid strain diluent to obtain cordyceps cultivation soil mixture; wiping the silkworm larvae at four to five instar with alcohol, using a disposable syringe to suck the primary liquid strain, and injecting into the silkworm larvae; and inserting the inoculated silkworm larvae into the cordyceps cultivation soil mixture, maintaining heads of the larvae up, and harvesting after cultivation to obtain the cordyceps militaris fruiting body with the silkworm larvae as the hosts. The head of the cordyceps militaris fruiting body is more than 2cm in length, and content of effective ingredients is high.

Description

technical field [0001] The invention relates to a fruit body of Cordyceps militaris with silkworm larvae as host and a cultivation method thereof. Background technique [0002] 1. Cordyceps militaris (La.: Fr), also known as Cordyceps militaris, belongs to Ergotaceae, Ergotaceae, fungus, and is a rare medicinal fungus in the treasure house of Chinese medicine, Cordyceps sinensis. Studies have shown that the content of physiologically active substances and nutrients such as cordycepin, cordyceps polysaccharide, cordycepic acid, and SOD enzyme contained in Cordyceps militaris is higher than or close to that of Cordyceps sinensis, and has been approved as a new resource food by the Ministry of Health. The artificial culture of Cordyceps pupa is mostly with rice or chrysalis as medium, because the artificial culture of Bombyx mori Cordyceps does not really form the ossification in the worm body and the relevant reports of forming fruiting bodies with a length of more than 2cm. ...

AI Technical Summary

Problems solved by technology

[0008]5. Edible fungus 2007 (1) "Technical Test on Artificial Cultivation of Cordyceps militaris in Mt. Wild Cordyceps, separation and purification, artificial rejuvenation, 3-5 instar larvae and pupae of different silkworm larvae, 6 kinds of inoculation methods, such as live worm body spray liquid, mulberry leaf spray liquid, bacterial liquid injection, living body piercing, etc., are used. Infection effect of instar larvae inoculation, as can be seen from Table 2, 4th instar larvae can be infected by spraying bacteria liquid and feeding with mulberry leaves, and the infection rate is higher than that of 5th instar larvae, but the body of the larvae shrinks rapidly after being infected with bacteria, which is of no use value. After live inoculation, the 4th and 5th instar silkworms can be susceptible to bacteria, but the infection rate is lower than 50%, which has no development value. The sterilized inoculation of live insects can reach 100% infection rate, but the fruiting bodies formed are short. "Whole grass" is used, and the appearance is poor.

[0009] In the above 5 articles, the silkworm larvae did not form ossified body, so they shrunk rapidly after infection, which has no development and utilization value

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