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Extraction and purification process for cordycepin in cordyceps militaris link

A purification method, the technology of Cordyceps militaris, is applied in the field of deep processing of agricultural products to achieve the effect of less impurities, high activity and reduced separation difficulty

Inactive Publication Date: 2009-08-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0003] Macroporous adsorption resin is a newly developed separation technology at home and abroad in recent years, and it is widely used in the field of medicine (especially the refining of natural medicines), and is suitable for industrial production, but it has not yet been applied to the separation and purification of cordycepin. See the report, the present invention adopts macroporous adsorption resin separation technology to separate

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  • Extraction and purification process for cordycepin in cordyceps militaris link
  • Extraction and purification process for cordycepin in cordyceps militaris link

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Embodiment Construction

[0018] Extraction of cordycepin from Cordyceps militaris: The raw material of Cordyceps militaris Suke Pupa No. 1 (commercially available) is crushed, passed through a 40-100 mesh sieve, and then mixed with the extract solution deionized water at a material-liquid ratio of 1:20. On the cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.), ultrasonic extraction was carried out with an ultrasonic frequency of 90 kHz, the extraction temperature was 60 ° C, the extraction time was 30 minutes, and the temperature was 60 ° C. ) with 4000 rev / separation centrifugation to obtain the brown-yellow Cordyceps militaris extract. The invention adopts a low-temperature ultrasonic water extraction method to extract the cordycepin, which keeps the biological activity of the cordycepin to the greatest extent, and the extraction rate reaches 85%.

[0019] Separation and purification of cordycepin from Cordyceps militaris: put the extract on a macroporous resin column, the resin type is AB-...

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Abstract

The invention provides an extraction and purification method of cordycepin from Cordyceps militaris, and belongs to the deep-processing technical field of agricultural products. The method comprises the following steps: crushing Cordyceps militaris raw material, mixing the raw material with water at 1:20 raw material / water ratio, performing ultrasonic extraction, and performing centrifugal filtration on obtained extracting solution and pouring in a macroporous resin column at the adsorption flow of 1.8BV / hr; taking 15% methanol aqueous solution as an eluant to perform resin column elution, and decompressing and condensing obtained eluent at the temperature of 60 DEG C by a rotary evaporator to obtain Cordyceps militaris cordycepin concentrate. The method can help realize aqueous solution extraction of the cordycepin from the Cordyceps militaris raw material at low temperature, and the extraction rate reaches 85%; and polysaccharide and protein can be removed from the extract by macroporous resin separation and purification, thus the content of the cordycepin in the extract can reach 10%. The cordycepin in the Cordyceps militaris product has high activity, can be taken as a raw material for producing cordycepin monomer and can also be directly taken as an additive of health products and functional food, thus the cordycepin has wide market development prospect.

Description

1. Technical field [0001] The invention provides a method for extracting and purifying cordycepin from Cordyceps militaris, belonging to the technical field of deep processing of agricultural products. 2. Background technology [0002] Cordyceps militaris (Cordyceps militaris), also known as Northern Cordyceps, belongs to the same genus and heterogeneity with my country’s famous and precious Chinese medicinal material Cordyceps sinensis. Its cordycepin (cordycepin) content is (3.089±0.046)‰, which is about 3 to 6 times. Cordycepin is an adenosine structural analogue in Cordyceps sinensis, molecular formula C 10 h 13 0 3 N 5 . Cordycepin has been proven to have anti-tumor, anti-virus, anti-bacterial and other effects, as well as cytotoxicity and immune regulation. At present, the pure product price of cordycepin in the international market is 25,000 US dollars / g. Cordyceps militaris is widely cultivated in my country, but it is mostly used as medicine in the form of mycel...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08B01D15/00B01J20/26
Inventor 刘红锦闫征蒋宁周宏栋周余庆王萍李春阳王乃富
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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