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Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof

A liquid submerged fermentation and health beverage technology, which is applied in botany equipment and methods, food preparation, gardening, etc., can solve the problems of poor pertinence and waste, and achieve rich and comprehensive nutrition, long foam suppression time, and anti-aging Effect

Active Publication Date: 2013-10-02
黄雄
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different optimal cultivation and fermentation processes and conditions required by different edible fungi, the above methods are not very specific.
Especially for Cordyceps militaris, which is as healthy as Cordyceps sinensis, it is a waste if the main active ingredient, cordycepin, is not extracted to the maximum extent.

Method used

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  • Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Activation of strains

[0039] Preparation of mother culture medium: Weigh 200 grams of potatoes, wash them, cut them into small pieces, add distilled water, boil them for 30 minutes, take their boiled juice, 30 grams of glucose, 20 grams of corn flour, 3 grams of peptone, and 1.2 potassium dihydrogen phosphate. grams, 0.5 grams of magnesium sulfate, 18-20 grams of agar, vitamin B 1 2-5 mg, add distilled water to make up to 1000 ml, pH is natural, autoclave at 121°C for 25-30 minutes, take it out and put it in a sterilized test tube, place the test tube on an inclined plane, and use it after the temperature drops below 30°C .

[0040] Select fresh and wild Cordyceps militaris with normal growth, robustness, and no pests and diseases as the separation material, and cut out 1 to 2 pieces with a size of 2mm after disinfection 3 The bacterium blocks of the bacteria were transferred to the test tube parent culture medium, and cultivated in a constant temperature incuba...

Embodiment 2

[0057] (1) Activation of strains

[0058] Select fresh and wild Cordyceps militaris with normal growth, robustness, and no pests and diseases as the separation material, and cut out 1 to 2 pieces with a size of 2mm after disinfection 3 The bacterium blocks of the bacteria were transferred to the test tube parent medium, and cultivated in a constant temperature incubator at 23-25°C for 9 days, and white Cordyceps militaris cells were seen.

[0059] (2) Primary shake flask seed culture

[0060] Cut 1-2 pieces from the activated Cordyceps militaris with a size of 2cm 2 The thin bacterial block was transferred to the Erlenmeyer flask equipped with the first-level shake flask medium, and was cultured statically for 2 days, then placed on a shaker, and cultured for 4 days at 23-25°C and 100 rpm for 4 days, and obtained A primary shake flask seed solution for a large number of mycelia.

[0061] (3) Secondary shake flask seed culture

[0062] Inoculate the first-level shake flask ...

Embodiment 3

[0070] (1) Activation of strains

[0071] Select fresh and wild Cordyceps militaris with normal growth, robustness, and no pests and diseases as the separation material, and cut out 1 to 2 pieces with a size of 2mm after disinfection 3 The bacterium blocks of the bacteria were transferred to the test tube parent culture medium, and cultivated in a constant temperature incubator at 23-25°C for 8 days, and white Cordyceps militaris cells were seen.

[0072] (2) Primary shake flask seed culture

[0073] Cut 1-2 pieces from the activated Cordyceps militaris with a size of 2cm 2 The thin bacterial block was transferred to the Erlenmeyer flask equipped with the first-level shake flask medium, cultured statically for 2 days, then placed on a shaker, and cultured for 4 days at 23-25°C and 110 rpm for 4 days, and obtained A primary shake flask seed solution for a large amount of mycelium.

[0074] (3) Secondary shake flask seed culture

[0075] Inoculate the first-level shake flask...

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Abstract

The invention discloses a cordyceps militaris health-care beverage prepared by liquid submerged fermentation and a preparation method thereof. The preparation method comprises the following steps: sequentially performing culture activation, shake culture, seeding tank cultivation and fermentation tank cultivation on cordyceps militaris, then achieving high-temperature extraction of effective components and thromboplastin killing to obtain fermentation liquor, and finally conducting filtering, blending, sterilizing, subpackaging and other procedures to obtain the health-care beverage product. The cordyceps militaris health-care beverage is prepared by adopting the liquid submerged fermentation technology, is simple in process and free from influences of seasons and weather, meets the large-scale periodic production requirement, is short in producting cycle, low in cost, and excellent and stable in product quality, and has unique heavy fragrance, efficiency and properties of cordyceps militaris, thereby obtaining very wide market prospect.

Description

technical field [0001] The invention relates to an edible mushroom health drink and a preparation method thereof, in particular to a Cordyceps militaris health drink prepared by liquid submerged fermentation and a preparation method thereof. Background technique [0002] Edible mushrooms have high nutritional value. Edible fungi have comprehensive amino acid composition and high utilization rate, and are recognized as "very good protein source" in the world. Edible fungi are also an important source of natural food vitamins. Among them, the content of vitamin B, which is easily deficient in the human body, is richer than that of ordinary vegetables, and the content of vitamin B2 is higher than that of meat and cheese. It can prevent pernicious anemia, improve nerve function and effectively reduce blood lipids. The mineral content in edible fungi is twice that of vegetables, and higher than that of beef and mutton. Edible fungus does not contain starch and has low fat conte...

Claims

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Application Information

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IPC IPC(8): A23L2/38A23L1/28A23L1/29A01G1/04A23L31/00
Inventor 黄雄
Owner 黄雄
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