Membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, bitter gourd polypeptide protein extract and application of bitter gourd polypeptide protein extract

A technology for the separation of balsam pear polypeptides and membranes, which is applied in the fields of peptide/protein components, plant protein processing, protein food components, etc., can solve the problems such as the need to improve the therapeutic effect of diabetes, the low content of polypeptide and protein, and the unclear molecular weight distribution, etc. Natural activity, low process energy consumption, short cycle effect

Inactive Publication Date: 2018-01-19
李玉保 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although scientific and technical personnel have realized that the bitter melon polypeptide extract extracted from bitter melon can be used to treat diabetes, and many bitter melon extracts and corresponding products have also appeared on the market, but there are some problems at the same time: one is the above-mentioned literature and related The bitter melon polypeptide The preparation method is mostly obtained by various enzymatic hydrolysis reactions or alkali extraction and acid precipitation combined with other production methods such as precipitation. The preparation time period is long or the operating temperature of some steps is high. The protein content of the polypeptide is not high, rarely exceeding 10%, and the molecular weight distribution is not high. clear
Naturally active bitter gourd polypeptide is the key component to effectively regulate blood sugar metabolism, therefore, the existing bitter gourd polypeptide protein extract and corresponding products have to be improved for the treatment of diabetes

Method used

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  • Membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, bitter gourd polypeptide protein extract and application of bitter gourd polypeptide protein extract
  • Membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, bitter gourd polypeptide protein extract and application of bitter gourd polypeptide protein extract
  • Membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, bitter gourd polypeptide protein extract and application of bitter gourd polypeptide protein extract

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Experimental program
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Embodiment 1

[0058] This embodiment provides a bitter melon polypeptide protein extract, which is prepared by the following method:

[0059] Fresh balsam pear with long growth period, yellowish green color and plump balsam pear seeds is selected as the raw material. The raw material of balsam pear is washed with tap water first, and then washed with electrolytic high-concentration ozone water. The ozone concentration in the cleaning water is 15mg / L, and the water flow rate is 300L / h, to reduce pesticide residues and microbial residues.

[0060] Beating and crushing the washed bitter gourd raw material into a liquid material passing through a 20-mesh sieve.

[0061] Pour the beaten and crushed bitter gourd raw materials into an ultrasonic-assisted extraction tank, add 8 times the mass of purified water at 50°C, use buffer salt to adjust the pH of the system to ≈7, and perform ultrasonic-assisted countercurrent extraction for 3 times, each time for 30 minutes.

[0062] Use a high-speed tub...

Embodiment 2

[0068] This embodiment provides a bitter melon polypeptide protein extract, which is prepared by the following method:

[0069] Fresh bitter gourd with long growth period, yellowish green color and plump bitter gourd seeds is selected as raw material. The raw material of bitter gourd is washed with tap water first, and then washed with electrolytic high-concentration ozone water. The ozone concentration in the water is 20mg / L, and the water flow rate is 450L / h. , to reduce pesticide residues and microbial residues.

[0070] Beating and crushing the cleaned bitter gourd raw materials into liquid materials passing through a 20-mesh sieve.

[0071] Pour the beaten and crushed bitter gourd raw materials into an ultrasonic-assisted extraction tank, add 5 times the mass of purified water at 38°C, use buffer salt to adjust the pH of the system to ≈6.7, and perform ultrasonic-assisted countercurrent extraction twice, each time for 60 minutes.

[0072] Use a disc centrifuge for centri...

Embodiment 3

[0078] This embodiment provides a bitter melon polypeptide protein extract, which is prepared by the following method:

[0079] Choose dried bitter gourd or sliced ​​bitter gourd with greenish yellowish color and plump flesh as raw material, and crush the raw material of bitter gourd into coarse powder passing through a 20-mesh sieve.

[0080] Pour the crushed bitter gourd raw material into an ultrasonic-assisted extraction tank, add 10 times the mass of purified water at 52°C, use buffer salt to adjust the pH of the system to ≈6.8, and perform ultrasonic-assisted countercurrent extraction twice, each time for 45 minutes.

[0081] Use a high-speed tubular centrifuge for centrifugation, and remove the slag to obtain a centrate.

[0082] The centrifugate was filtered through a ceramic microfiltration membrane system with a molecular weight cut-off of 0.2 μm, the operating temperature was 35° C., and the pressure was 0.5 MPa to obtain the permeate of the microfiltration membrane....

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Abstract

The invention provides a membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, a bitter gourd polypeptide protein extract and an application of thebitter gourd polypeptide protein extract, and relates to the technical fields of deep processing of bitter gourds and polypeptide extraction and purification. The membrane separation and purificationtechnical technology comprises the following steps of cleaning bitter gourds as raw materials, performing pulping, performing crushing, performing leaching, performing centrifuging, and removing residues so as to obtain centrifugate; filtering the centrifugate through a micro filtration membrane system so as to obtain micro filtration membrane penetration fluid; performing separation and purification on the penetration fluid on two coiling type ultra filtration membrane purification systems of different molecular weight so as to obtain ultra filtration membrane trapped fluid; concentrating thetrapped fluid through a high-pressure reverse osmosis membrane so as to obtain concentrate of which the solid matter content is greater than or equal to 30%; and drying the concentrate. The new technical technology effectively guarantees the natural activity of the bitter gourd polypeptide protein and the content of the bitter gourd polypeptide protein being higher than 25%; and the prepared extract contains a great amount of bitter gourd polypeptide having the function of adjusting and controlling the metabolism of blood sugar and having specific molecular weight and sequence and has good treatment effects on diabetes, and the bitter gourd polypeptide protein extract can be made into foods for special dietary uses, health-care foods or drugs for treating the diabetes.

Description

technical field [0001] The invention relates to the technical field of edible agricultural product processing, and in particular to a membrane separation and purification process for preparing bitter gourd polypeptide protein, bitter gourd polypeptide protein extract and application thereof. Background technique [0002] Bitter gourd, also known as bitter gourd, belongs to Cucurbitaceae. It is an annual climbing plant. The fruit is oblong and yellow when ripe. There are many irregular tumor-like protrusions on the surface, and the seeds are hidden in the fleshy fruit. Bitter gourd is bitter in taste and cold in nature. It is a vegetable with both medicinal and edible uses. According to "Compendium of Materia Medica" and "Southern Yunnan Materia Medica", bitter gourd has the functions of clearing away heat and relieving heat, nourishing blood and nourishing qi, tonifying kidney and spleen, nourishing liver and improving eyesight; the content of vitamin C in bitter gourd is hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23J1/00A23J1/14A23J3/14A61K36/42A61K38/16A61P3/10
Inventor 何静仁李玉保
Owner 李玉保
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