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Composite microbial inoculum for degrading high-ammonia nitrogen sewage and application of composite microbial inoculum to sewage treatment

A compound bacterial agent, high ammonia nitrogen technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, polluted waterway/lake/pond/river treatment, etc. Complicated process, serious secondary pollution and other problems, to achieve the effect of good protection of bacteria, simple preparation process and short action time

Active Publication Date: 2019-09-27
新疆天康饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the complex process flow, serious secondary pollution, and difficulty in maintaining the advantages of bacteria caused by pretreatment in the current traditional photosynthetic bacteria sewage treatment process, a method that can effectively treat high ammonia nitrogen sewage is proposed.

Method used

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  • Composite microbial inoculum for degrading high-ammonia nitrogen sewage and application of composite microbial inoculum to sewage treatment
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  • Composite microbial inoculum for degrading high-ammonia nitrogen sewage and application of composite microbial inoculum to sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Degradation of high-ammonia-nitrogen wastewater composite bacteria

[0039] The present invention provides a preparation method of a composite bacterial agent for degrading high ammonia nitrogen sewage, including the following steps:

[0040] (1) Rhodobacter capsulatus 3Z CGMCC No.17568, Rhodobacter sphaeroides, Rhodopseudomona spalustris and Rhodobacter johrii stored at 4°C under sterile conditions They were respectively inoculated into sterilized liquid RCVBN medium, placed in an incubator with a light intensity of 3000-5000Lx at 25°C, and allowed to stand for 72 hours to obtain Rhodobacter sphaeroides fermentation broth, Rhodobacter capsulatus 3Z fermentation broth, and swamp respectively. Rhodopseudomonas fermentation broth, Bacillus subtilis stored at 4°C was inoculated into sterilized LB medium under aseptic conditions, and placed in an incubator at 25°C for 72h to obtain Bacillus subtilis. Bacillus fermentation broth;

[0041] (2) The Rhodobacter capsulatus...

Embodiment 2

[0045] Example 2: Separation and screening of Rhodobacter capsulata 3Z

[0046] 1. Separation and purification

[0047] Weigh 5g of glacier front soil into a 250ml Erlenmeyer flask, add sterilized liquid RCVBN medium to the mouth of the bottle, seal with sterile liquid paraffin to isolate the air, and put it in a 25℃ light intensity of 3000-5000Lx Cultivate statically in an incubator. Observe whether the culture medium in the bottle appears obvious red, pink or yellow. After the culture medium changes color, repeat the transfer 2-3 times until the photosynthetic bacteria in the bottle dominate, then proceed to the next step of plate separation and purification.

[0048] Using the double-layer agar plate method, the enrichment solution was gradually diluted by 10 times, and the 10 -5 , 10 -6 Use a pipette to pipette 100 μL of the two gradient dilutions onto the solid RCVBN plate, spread it evenly with a spreading rod, and place it at room temperature for 30 minutes. After the bacter...

Embodiment 3

[0058] Example 3: Performance of Bacillus subtilis strains

[0059] 1. Low temperature growth test

[0060] As attached figure 2 As shown, when the culture temperature is 20℃, the strain begins to enter the exponential phase at about 12h, and the growth of the strain at 25℃ for 26h is equivalent to the amount of bacteria cultured at 20℃, indicating that the Bacillus subtilis strain is cultured at 25℃ and 20℃ The amount of bacteria at 26h is equivalent.

[0061] 2. Enzyme production characteristic test

[0062] Bacillus subtilis was tested for the production of amylase, protease and cellulase. The initial screening of amylase activity was carried out by the Lucca's iodine solution dip plate method, the protease activity was adopted by the Coomassie brilliant blue colorimetric method; the initial screening of cellulase activity was carried out by Congo red Dip plate detection method. The results showed that this strain can produce obvious protease and amylase, but not cellulase.

[00...

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Abstract

The invention discloses a method capable of effectively treating high-ammonia nitrogen sewage, and aims at solving the problems of complicated process flow, serious secondary pollution, difficulty in maintaining strain advantages and the like caused by pretreatment in the traditional photosynthetic bacteria sewage treatment process at present and aims to provide a composite microbial inoculum for degrading the high-ammonia nitrogen sewage. The composite microbial inoculum for degrading the high-ammonia nitrogen sewage is obtained by mixing Rhodobacter capsulatus 3Z, Rhodobacter sphaeroides fermentation liquor, Rhodopseudomonas palustris fermentation liquor, Rhodobater johrii fermentation liquor and Bacillus subtilis fermentation liquor. The preparation method has simple process, can efficiently convert inorganic nitrogen into organic nitrogen, and obviously improves outstanding practicability of sewage treatment effect.

Description

Technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a composite bacterial agent for degrading high ammonia nitrogen sewage and the technical field of its application in sewage treatment. Background technique [0002] In recent years, due to frequent human activities, more and more sewage discharges. Among them, the pollution caused by high-concentration ammonia-nitrogen sewage has increased. The discharge of untreated or incompletely-treated high-ammonia sewage will cause the dissolved oxygen in the water to continue to decline, the eutrophication of the water is increasingly serious, and the water quality is deteriorated. In an environment with excessive ammonia nitrogen, microorganisms such as algae and bacteria die in large numbers. In the actual industrial wastewater treatment process, high ammonia nitrogen wastewater contains more pollutants, including biodegradable organic matter and difficult-to-degrade pollutants. In...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/125C02F101/16C02F101/10
CPCC02F3/34C02F2101/105C02F2101/16C02F2101/166C02F2103/007C12N1/20
Inventor 樊振曾军马贵军娄恺
Owner 新疆天康饲料有限公司
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