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Method for culturing photosynthetic bacteria agent

A culture method and technology of photosynthetic bacteria agent, which is applied in the field of valve manufacturing, can solve the problems of low efficiency of naturally growing photosynthetic bacteria agent, and achieve the effect of good quality and high efficiency

Inactive Publication Date: 2018-02-02
成都市新津胤春生物科技有限责任公司
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  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the efficiency of naturally growing photosynthetic bacteria inoculum is extremely low, and it is necessary to improve the cultivation efficiency of inoculum

Method used

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with specific embodiments.

[0013] A photosynthetic bacteria agent cultivation method is characterized in that, comprises the following steps:

[0014] A. Culture medium preparation: Prepare the purified water to be weakly alkaline, with a pH value of 7.5-8.5, and add 0.3% peptone, 0.3% sodium citrate, 0.2% yeast extract, and 0.2% sodium bicarbonate to the purified water according to the mass percentage, Sodium chloride 0.2%, ammonium chloride 0.2%, magnesium sulfate heptahydrate 0.2%, potassium dihydrogen phosphate 0.2%, the balance is pure water;

[0015] B. Selection of strains: select effective live strains, and the strains include Rhodopseudomonas sphaericus and Rhodopseudomonas paluscens, mixed according to the mass percentage of 1:1.5; wherein Rhodopseudomonas palustris is divided into double-layer plates Cultivate until a red single colony appears;

[0016] C. Seed liquid culture: Ferment an...

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Abstract

The invention discloses a method for cultivating a photosynthetic bacteria agent, comprising the following steps: A. Culture medium preparation: prepare pure water to be slightly alkaline, with a pH value of 7.5-8.5, and add 0.3% peptone, lemon Sodium acid 0.3%, yeast extract 0.2%, sodium bicarbonate 0.2%, sodium chloride 0.2%, ammonium chloride 0.2%, magnesium sulfate heptahydrate 0.2%, potassium dihydrogen phosphate 0.2%, and the balance is pure water; B. Selected strains: select effective live strains, and the strains include Rhodopseudomonas sphaericus and Rhodopseudomonas paluscens, mixed according to the mass percentage of 1:1.5; Among them, Rhodopseudomonas palustris is cultured by double-layer plate culture method Cultivate until the red single bacterium colony appears; C. Seed liquid culture: the mixed live strain that above-mentioned B step gained is fermented and cultivated, and the temperature of cultivation is 27-32 ℃, and illumination condition is 2500Lx~3000Lx; Adopt the present invention can effectively improve Cultivation efficiency of photosynthetic bacteria inoculum.

Description

technical field [0001] The invention relates to the technical field of valve manufacture, in particular to a method for cultivating photosynthetic bacteria agents. Background technique [0002] Photosynthetic bacteria (PSB for short) are prokaryotic organisms that first appeared on the earth, are ubiquitous in nature, and have a primitive light energy synthesis system. Gram-negative bacteria with the ability to use light as energy, can use organic matter, sulfide, ammonia, etc. in nature as hydrogen donors and carbon sources for photosynthesis under anaerobic light or aerobic dark conditions . Photosynthetic bacteria are widely distributed in soil, paddy fields, swamps, lakes, rivers and seas in nature, and are mainly distributed in anoxic areas where light can penetrate in aquatic environments. [0003] However, the efficiency of naturally growing photosynthetic bacteria inoculum is extremely low, and it is necessary to improve the cultivation efficiency of inoculum. Co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 王品孟黄丽
Owner 成都市新津胤春生物科技有限责任公司
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