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Rhodobacter capsulatus strain and application thereof

A technology for encapsulated rhodobacteria and water bodies, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of less photosynthetic bacteria in benzene series and formaldehyde, and achieve a wide range of effects

Active Publication Date: 2017-07-28
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few reports on photosynthetic bacteria capable of degrading benzene series and formaldehyde at home and abroad

Method used

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  • Rhodobacter capsulatus strain and application thereof
  • Rhodobacter capsulatus strain and application thereof
  • Rhodobacter capsulatus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Isolation, purification and identification of Rhodobacter capsularis DC-1

[0036] 1. After removing impurities from the sewage collected from the entrance of Dianchi Wetland Park in Kunming, pour it into 500mL enrichment culture solution (formula NaCl 2.5 g, MgSO 4 ·7H 2 O 0.05 g, NH4Cl 1.0 g, NaHCO 3 2.5 g, KH 2 PO 4 2.75 g, CH 3 COONa 6g, yeast powder 2g, distilled water 1000mL, pH value 7.0) in a mineral water bottle, mix well, seal; finally place at 28°C, 60W incandescent light culture for 7-8 days; wait for the culture solution to turn red or blood red, suck out 50mL of red liquid, pour it into a mineral water bottle filled with fresh enriched culture medium, and enrich and cultivate continuously for 3 times; use RCVBN culture medium (the formula is CH 3 COONa 5.0g, CH 3 CH 2 COONa 3.0g, NaCl 2.5g, MgSO 4 ·7H 2 O 1.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 1.5g, K 2 HPO 4 0.6g, CaCl 2 0.05g, MnSO 4 5mg, FeSO 4 5mg, yeast extract 0.5g...

Embodiment 2

[0055] Embodiment 2: the optimization of photosynthetic bacteria culture condition

[0056] 1. Determination of temperature Add 20% seed liquid into a 25mL anaerobic tube, and use liquid medium (the formula is CH 3 CH 2 COONa 5g / L, (NH 4 ) 2 SO 4 2.0g / L, MgSO 4 •7H 2 O 0.2g / L, NaH 2 PO 4 •H 2 O 0.5g / L, K 2 HPO 4 0.66 g / L, CaCl 2 • 2H 2 O 0.1 g / L. ) was filled with anaerobic tubes, and under the condition of pH 7, the culture temperature was set to 20, 25, 30, and 35°C respectively; the light culture was 7 days, and the experiment was repeated three times, and the bacteria culture solution was measured every 24 hours. The OD value at 375nm was used to determine the optimal culture temperature. Image 6 is the effect of temperature on the growth of strain DC-1, by Image 6 It can be seen that the optimal culture temperature of strain DC-1 is 30°C.

[0057] 2. Determination of pH Add 20% seed liquid into a 25mL anaerobic tube, and use a liquid medium (the formula...

Embodiment 3

[0061] Embodiment 3: the ability of bacterial strain DC-1 to purify benzene series

[0062] The liquid culture of the strain DC-1 with a concentration of 20% was inoculated in the liquid medium containing 2mM, 4mM, and 6mM benzene respectively, and cultured at 30°C under the light of 60W incandescent lamp for 0, 1, 2, 3, 4, 5, 6 , 7, and 8 days, the experiment was set for 3 repetitions, and the cell concentration and residual liquid benzene concentration of strain DC-1 were measured at each time point; the specific steps for the determination of benzene content were as follows:

[0063] 1. Preparation of formaldehyde-sulfuric acid solution: add 0.10 mL of 10% formaldehyde solution to 100 mL of high-grade concentrated sulfuric acid, mix, store in ice cold (prepared when used);

[0064] (In the presence of sulfuric acid, benzene series reacts with formaldehyde to form a yellow-brown diphenylmethane polymer. The maximum absorption wavelength of this colored compound is 465 nm. Us...

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Abstract

The invention discloses a rhodobacter capsulatus strain DC-1 and application thereof. The collection number of the strain in China Center for Type Culture Collection is CCTCC NO: M2016676. The strain disclosed by the invention refers to photosynthetic bacteria capable of efficiently degrading benzene series and formaldehyde, can be used for treating sewage polluted by the benzene series and formaldehyde and is high in tolerance concentration of the benzene series and excellent in degradation effect; and moreover, the strain has capacity of removing the formaldehyde, the culture conditions of the strain are simple, and industrialized implementation is easily realized.

Description

technical field [0001] The invention relates to a photosynthetic bacterium capsular rhodobacter and its application in the purification of formaldehyde and benzene series, belonging to the field of sewage treatment. Background technique [0002] Benzene series are common pollutants in the environment. They are carcinogenic, teratogenic, and mutagenic. They are listed as monitoring items in the national water quality standards for drinking water. Long-term exposure to benzene may cause chronic poisoning, leading to aplastic anemia and leukemia. Its main pollution comes from waste gas discharged from industries such as decoration materials and paints, and it is used as a disinfectant in the waste water produced by medical and sanitation and aquatic industries. Formaldehyde (HCHO), also known as "formaldehyde", is a colorless, easily soluble, slightly irritating gas with high biological activity and potential carcinogenicity at room temperature. Concealment features. The har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/30C02F101/34
CPCC02F2101/30C02F2101/34C12R2001/01C12N1/205
Inventor 李昆志雷玉珠操璟璟包燚陈丽梅徐慧妮
Owner KUNMING UNIV OF SCI & TECH
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