Photosynthetic bacterium capsulated rhodobacter strain, microbial agent, preparation method and application thereof

A technology of rhodobacter capsularis and photosynthetic bacteria, which is applied in the field of bacterial agents and its preparation, and strains of rhodobacter capsularis of photosynthetic bacteria, which can solve the problems of difficulty and high cost in the control of rice bacterial blight, and achieve drug resistance , low cost, and simple cultivation process

Active Publication Date: 2020-10-16
HUNAN PLANT PROTECTION INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a photosynthetic bacterium Rhodobacter capsularis strain, bacterial agent and its preparation method and application, so as to solve the technical problem of difficulty and high cost in the prevention and treatment of rice bacterial blight in the current rice growth process

Method used

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  • Photosynthetic bacterium capsulated rhodobacter strain, microbial agent, preparation method and application thereof
  • Photosynthetic bacterium capsulated rhodobacter strain, microbial agent, preparation method and application thereof
  • Photosynthetic bacterium capsulated rhodobacter strain, microbial agent, preparation method and application thereof

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preparation example Construction

[0035] According to another aspect of the present invention, there is also provided a method for preparing a biocontrol agent of the above-mentioned photosynthetic bacteria Rhodobacter capsular strain, comprising the following steps: S1, activation: the preserved species of photosynthetic bacteria Rhodobacter capsular strain Cultivate according to the double-layer plate culture method until the red single colony appears; S2, seed culture: insert the red single colony cultivated in step S1 into a serum bottle, and carry out seed culture to logarithm with photosynthetic bacteria liquid medium Obtain the bacterial liquid in the growth phase; S3, production culture: inoculate the bacterial liquid in the step S2 with an inoculum amount of 3% to 6% of the total volume of the photosynthetic bacteria strain production medium, and cultivate to the logarithmic growth phase to obtain the photosynthetic bacteria capsulated rhodobacter strain of biocontrol agents.

[0036] In this embodime...

Embodiment 1

[0052]Use the inhibition zone method to measure the inhibition of photosynthetic bacteria strain Rhodobacter capsulata A12 to the pathogenic bacteria that cause rice bacterial blight. Get 0.1ml of pathogenic bacteria and spread it on the LB flat plate, put a sterilizing steel ring in the center, and place it on the steel plate. Add 0.05ml of photosynthetic bacteria liquid to the circle, incubate at 30°C for 7 days, and observe whether there is a zone of inhibition.

Embodiment 2

[0054] Under greenhouse conditions, the 5×10 6 cfu / mL biocontrol agent of photosynthetic bacteria Rhodobacter capsularis A12 strain. Soak the rice seeds germinated to white at 30°C for 1 hour, sow the treated rice seeds in pots, and keep the seedlings at 28°C during the identification period. Cultivate in a greenhouse under 10h / d light conditions, and record the plant height regularly. Three representative plants were selected to measure the plant height and wet weight respectively. After the rice enters the seedling stage, the rice seedlings are inoculated with Xanthomonas oryzae, and the disease index is investigated 7 days after the inoculation and the control effect is calculated.

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Abstract

The invention discloses a strain of photosynthetic bacterium Rhodobacter capsulatus, bacterial agent and its preparation method and application. Culture Collection Center, the deposit number is CCTCC No: M 2018604. The cultivation process of the photosynthetic bacterium Rhodobacter capsulata A12 is simple, the culture time is short, and the cost is low, and the photosynthetic bacterium Rhodobacter capsulata strain can be prepared by a simple, fast and low-cost operation method to obtain a biocontrol agent for the prevention and control of rice bacterial blight. Bacteria and biological control fermentation liquid.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a strain of photosynthetic bacteria Rhodobacter capsularis. In addition, the present invention also relates to a microbial agent comprising the above-mentioned strain of the photosynthetic bacteria Rhodobacter capsulatus and its preparation method and application. Background technique [0002] Rice bacterial blight is one of the important diseases in rice production. Rice bacterial blight, commonly known as white leaf blast, is one of the main diseases of rice and is a quarantine disease. Rice bacterial blight mainly damages the leaves, causing the plants to wither and die, and has a great impact on rice yield. Once the rice bacterial blight occurs, it can generally reduce the yield by about 10%, and if it is serious, it can reduce the yield by 50% to 60%, or even 90%. %. The disease is caused by bacteria, the pathogen is Xanthomonas oryzae (Xanthomonasoryzae). The prima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 苏品刘勇张德咏程菊娥陈岳李成刚
Owner HUNAN PLANT PROTECTION INST
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