Rhodobacter capsulatus and application thereof

A technology for encapsulated red bacteria and selenium inoculants, applied in the directions of application, bacteria, biochemical equipment and methods, etc., can solve the problem that the utilization rate of selenium elements cannot be guaranteed in tea leaves, and can meet the requirements of selenium supplementation health care services and improve selenium enrichment. , the effect of promoting conversion and absorption

Active Publication Date: 2012-08-01
福建省安野牛业食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After spraying selenium fertilizer on the leaves, the selenium content in the tea can be rapidly increased. The selenium content of the tea leaves is directly proportional to the amount of selenium fertilizer sprayed. Whe

Method used

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  • Rhodobacter capsulatus and application thereof
  • Rhodobacter capsulatus and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, acquisition and preservation of bacterial strain AYGHJ-16

[0025] 1. Obtaining the strain AYGHJ-16S

[0026] Photosynthetic bacteria that can tolerate high concentration of sodium selenite and transform inorganic selenium into organic selenium were isolated from the bottom mud of Fuzhou West Lake by means of enrichment and selective identification culture techniques. A strain was obtained, which was named strain AYGHJ-16S. The selenium-rich amount of strain AYGHJ-16S is 150ug / ml.

[0027] 2. Identification of strain AYGHJ-16S

[0028] 1. Morphological characteristics

[0029] Gram-negative bacteria;

[0030] The bacteria are short-rod or egg-shaped, with a size of about (0.6-1.2) μm-(2.0-2.2) μm;

[0031] The colonies are smooth, round and protruding under light conditions;

[0032] Colonies are red under light conditions and colorless under dark conditions.

[0033] 2. Molecular characteristics

[0034] The sequencing result of 16S rDNA is shown ...

Embodiment 2

[0040] Embodiment 2, the optimization of the fermented components and technological conditions of capsulated rhodobacter AYGHJ-16

[0041] Through single factor and orthogonal experiments, the following medium composition parameters were determined:

[0042] Glucose is the best carbon source in the medium, and the highest biomass and selenium content can be obtained when adding 4-5%;

[0043] Peptone and urea are the compound nitrogen sources in the medium, and the addition amounts are: 0.5% peptone and 0.9% urea;

[0044] Yeast extract is an auxiliary nitrogen source in the medium, and the addition amount is 0.4%;

[0045] Sodium selenite is selected as the inorganic selenium source in the medium, and the addition amount is 120ug / mL.

[0046] Through the uniformly designed shake flask fermentation experiment, the following culture condition parameters were determined:

[0047] A 250mL shake flask was used, with a liquid volume of 120mL, a culture temperature of 28°C, a pH ...

Embodiment 3

[0049] Embodiment 3, the preparation of selenium-enriched bacterial agent

[0050] One, the preparation of selenium-enriched bacterial agent

[0051] Preparation method of fermentation medium (pH7.0): take 120mg sodium selenite, 40g glucose, 7.5g peptone, 9g urea, 5g yeast extract, 0.4g NaHCO 3 , 1g NH 4 Cl, 0.2g K 2 HPO 4 、2.0CH 3 COONa, 0.2gMgSO 4 ·7H 2 O, 2.0g NaCl and 10mL trace element solution were dissolved in distilled water and adjusted to 1000mL. The inorganic selenium concentration of the fermentation medium was 120mg / L. In practical applications, the pH of the fermentation medium may be 6.7-7.2.

[0052] The fermentation medium was sterilized at 115 °C for 20 min.

[0053] The preparation method of trace element solution: take 0.5g H 3 BO 3 , 400mg MnSO 4 , 60mg ZnSO 4 ·7H 2 O, 180mgNaMoO 4 2H 2 O and 10mg Ca(NO 3 ) 2 2H 2 O, dissolved in distilled water and adjusted to 250mL.

[0054] A 200L fermenter was adopted, and the fermenter was modified...

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Abstract

The invention discloses rhodobacter capsulatus and application thereof. The collection number of the rhodobacter capsulatus AYGHJ-16 is CCTCC NO: M 2011377; the rhodobacter capsulatus belongs to photosynthetic bacteria; and through the rhodobacter capsulatus, certain organic small molecules (such as organic acid and amino acid) can be converted into growth-stimulating substances and anti-virus substances by using light energy, so that growth of plants is stimulated, and the disease-resistant immunity of the plants is improved. Moreover, through the photosynthetic bacteria, product feedback inhibition of heterotrophic microbes can be released by using organic acid produced by metabolism of the heterotrophic microbes in soil, so that a large quantity of heterotrophic microbes are propagated, and conversion and absorption of certain inorganic substances in the soil are promoted; and the photosynthetic bacteria have strong nitrogen fixation and carbon fixation effects, so more inorganic substances can be provided for plants. The selenium-rich microbial agent can improve the micro-ecological environment of the soil, so that the disease resistance of the plants and internal absorption of various mineral elements and rare elements (such as selenium) are improved, and the selenium-rich requirements of the plants are met.

Description

technical field [0001] The invention relates to a strain of capsulated rhodobacter and its application. Background technique [0002] Selenium was discovered by Swedish chemist Berzelius in 1817 in the tailings of sulfuric acid production and was considered a poisonous element at the time. In 1957, Schwarz and others proved for the first time that selenium is an essential nutrient element for animals, and people had some understanding of the nutritional and health effects of selenium. In 1973, after Rotmck et al. discovered and confirmed that selenium is a component of glutathione peroxidase in animals and humans, the importance of selenium has received unprecedented attention. In 1973, the World Health Organization announced that selenium is an essential element of life for the human body. Medical research in recent years has shown that selenium deficiency can cause more than 40 kinds of diseases, especially the following diseases: (1) Keshan disease, Kaschin-Beck disease...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCY02P60/12
Inventor 黄钦耿黄元君施碧红刘建明翁雪清吴伟斌毛宁黄祥峰施巧琴吴松刚
Owner 福建省安野牛业食品有限公司
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