Method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae employing bioconversion technology

A technology of phenolic acids and salvia miltiorrhiza, applied in the field of resource utilization of salvia phenolic acid components, aboveground stems and leaves of salvia miltiorrhiza, to achieve the effects of improving resource utilization efficiency, increasing industrial economic benefits, and good ecological benefits

Inactive Publication Date: 2015-05-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are reports on relevant research and development, the aboveground resources have not been effectively utilized. Therefore, the

Method used

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  • Method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae employing bioconversion technology
  • Method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae employing bioconversion technology
  • Method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae employing bioconversion technology

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Experimental program
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Effect test

Embodiment 1

[0037] In the method for preparing salvianolic acid components from aboveground stems and leaves of Salvia miltiorrhiza, it comprises the following steps:

[0038] (1) Take 50g dry powder of above ground stems and leaves of Salvia miltiorrhiza, place in a 1000ml Erlenmeyer flask, accurately add 500ml of pure water, accurately weigh it, soak it for 16h, then ultrasonicate it at 30°C and 100KHz for 30min, weigh to make up for the weight loss, Filter, repeat the extraction three times, combine the filtrates, and concentrate the solution to a dry powder. Take an appropriate amount of dry powder, dissolve it in water to a certain volume, pass through a 0.22 μm membrane, and set the volume to a certain volume. Under sterile conditions, inoculate 1 / 20 volume of Rhodobacter sphaeroides bacteria solution and cultivate for 3-5 days to obtain Rhodobacter sphaericus Transformation of salvia miltiorrhiza culture fluid.

[0039] (2) Centrifuge at 3000r / min for the culture solution of Salvi...

Embodiment 2

[0041] In the method for preparing salvianolic acid components from aboveground stems and leaves of Salvia miltiorrhiza, it comprises the following steps:

[0042] (1) Take 100g dry powder of above ground stems and leaves of Salvia miltiorrhiza, place in a 1000ml Erlenmeyer flask, accurately add 500ml of pure water, accurately weigh it, soak it for 10h, then ultrasonicate for 30min at 30°C and 100KHz, weigh to make up for the weight loss, Filter, repeat the extraction three times, combine the filtrates, and concentrate the solution to a dry powder. Take an appropriate amount of dry powder, dissolve it in water to a certain volume, pass through a 0.22 μm membrane, and set the volume to a certain volume. Under sterile conditions, inoculate 1 / 10 of the volume of Rhodobacter sphaeroides bacteria solution and cultivate for 3 to 5 days to obtain Rhodobacter sphaericus Transformation of salvia miltiorrhiza culture fluid.

[0043] (2) Centrifuge at 2000r / min for the culture solution ...

Embodiment 3

[0045] In the method for preparing salvianolic acid components from aboveground stems and leaves of Salvia miltiorrhiza, it comprises the following steps:

[0046] (1) Take 50g dry powder of above ground stems and leaves of Salvia miltiorrhiza, put it in a 1000ml Erlenmeyer flask, accurately add 500ml of pure water, accurately weigh it, soak it for 15h, then ultrasonicate it at 30°C and 100KHz for 30min, weigh to make up for the weight loss, Filter, repeat the extraction three times, combine the filtrates, and concentrate the solution to a dry powder. Take an appropriate amount of dry powder, dissolve it in water to a certain volume, pass it through a 0.22 μm membrane, and set the volume to a certain volume. Under sterile conditions, inoculate 1 / 20 volume of Rhodopseudomonas palustris bacteria solution and cultivate for 3-5 days to obtain Rhodopseudomonas palustris transformed Salvia miltiorrhiza culture fluid.

[0047] (2) Centrifuge the Rhodopseudomonas palustris transforme...

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Abstract

The invention discloses a method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae. The method comprises the following steps: extracting overground stems and leaves of salviae miltiorrhizae, and carrying out bioconversion through photosynthetic bacteria including rhodopseudomonas palustris, green sulphur rhodop seudanonas palustris, rhodobacter sphaeroides, rhodobacter capsulatus and the like to prompte rise of salvianolic acid components in the stems and leaves, wherein the content of an active component salvianolic acid B is 3-4 times of that of the medicinal material, namely roots of the salviae miltiorrhizae, and the content of rosmarinic acid is dozens of times higher than that of the medicinal material salviae miltiorrhizae; and carrying out separation and purification through a macroporous resin column, so as to efficiently obtain the high-content extracts of phenolic acid components such as the salvianolic acid B, tanshinol, rosmarinic acid, ferulic acid and caffeic acid. The salvianolic acid components disclosed by the invention have good efficacies of resisting oxidation, protecting vascular endothelial cells, and promoting blood circulation to remove blood stasis, and can be applied to preparation of medicines or functional health products for preventing and treating cardiovascnlar and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a resource utilization method of aboveground stems and leaves of Salvia miltiorrhiza, in particular to a method for extracting and preparing salvianolic acid components from aboveground stems and leaves of Salvia miltiorrhiza in a certain growth period through photosynthetic bacteria transformation and the preparation thereof Components and uses of salvianolic acids. Background technique [0002] Salvia miltiorrhiza (Salvia miltiorrhizaBge.) is a plant of the genus Salvia in the Lamiaceae. It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Its roots and rhizomes are used as medicine called Danshen, which is bitter in taste and slightly cold in nature. It has the effects of promoting blood circulation and removing blood stasis, dredging menstruation and relieving pain, clearing the heart and eliminating troubles, cooling blood and el...

Claims

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Application Information

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IPC IPC(8): C12P1/04A61K36/537A61P9/00A23L1/29A23L33/00
Inventor 宿树兰段金廒江曙严辉郭盛钱大玮
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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